P5E1-Wasp

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$40.00
SKU: P5E1-Wasp
View product citations for antibody P5E1-Wasp on CiteAb

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Available: 160

DSHB Data Sheet

Catalog Fields

Antigen: Wasp
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: Fixation: 4% paraformaldehyde in PBS or standard embryo protocol.
Antigen Molecular Weight: Predicted: 57 kDa; Apparent: 75 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: WASp
Immunogen: Recombinant Wasp/GST fusion protein
Alternate Gene Names: CG1520, Wsp
Alternate Antibody Name: P5E1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: Q9VAT0 
Immunogen Sequence: Full length protein
Entrez Gene ID: 43402 
Additional Characterization:  
Antibody Registry ID: AB_2618392 
Additional Information: WASp: Wiskott-Aldrich Syndrome family protein.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5E1-Wasp was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P5E1-Wasp)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    IF References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    WB References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
    Rodal AA
    Proceedings of the National Academy of Sciences of the United States of America 113.38 (2016 Sep 20): E5552-61.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    All References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
    Rodal AA
    Proceedings of the National Academy of Sciences of the United States of America 113.38 (2016 Sep 20): E5552-61.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

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