P1C1-SCAR
Catalog Fields
Antigen: SCAR
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Depositors Institution: Fred Hutchinson Cancer Research Center
Host Species: mouse
Depositors Notes: Fixation for immunostaining: 4% paraformaldehyde in PBS or standard embryo protocol.
Positive Tested Species Reactivity: Drosophila melanogaster
Gene: SCAR
Antigen Molecular Weight: Multiple isoforms. Predicted: 9 and 67-74 kDa; Apparent: 85-90 kDa
Alternate Gene Names: CG4636, Wave, DWave, l(2)k03107
Immunogen: Recombinant SCAR/GST fusion protein
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Uniprot ID: Q9VKM2
Immunogen Sequence: Full length protein
Entrez Gene ID: 34519
Additional Information: SCAR: suppressor of cAR. SCAr is a Wiskott-Aldrich Syndrome family protein. SCAR is an actin nucleating factor that functions in lamellipodia formation. SCAR has been used as a marker for lamellipodia. SCAR is the main activator of Arp2/3 in fly embryos.
Antibody Registry ID: AB_2618386
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1C1-SCAR was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P1C1-SCAR)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
Parkhurst SM
Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.