P1C1-SCAR

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$40.00
SKU: P1C1-SCAR
View product citations for antibody P1C1-SCAR on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SCAR
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: Fixation for immunostaining: 4% paraformaldehyde in PBS or standard embryo protocol.
Antigen Molecular Weight: Multiple isoforms. Predicted: 9 and 67-74 kDa; Apparent: 85-90 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SCAR
Immunogen: Recombinant SCAR/GST fusion protein
Alternate Gene Names: CG4636, Wave, DWave, l(2)k03107
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: Q9VKM2 
Immunogen Sequence: Full length protein
Entrez Gene ID: 34519 
Additional Characterization:  
Antibody Registry ID: AB_2618386 
Additional Information: SCAR: suppressor of cAR. SCAr is a Wiskott-Aldrich Syndrome family protein. SCAR is an actin nucleating factor that functions in lamellipodia formation. SCAR has been used as a marker for lamellipodia. SCAR is the main activator of Arp2/3 in fly embryos.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1C1-SCAR was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P1C1-SCAR)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    IF References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology.
    Gonsalvez GB
    Biology open 4.11 (2015 Oct 12): 1448-61.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

    WB References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

    All References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology.
    Gonsalvez GB
    Biology open 4.11 (2015 Oct 12): 1448-61.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

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