P1C1-SCAR

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$40.00
SKU: P1C1-SCAR
View product citations for antibody P1C1-SCAR on CiteAb

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Available: 211

DSHB Data Sheet

Catalog Fields

Antigen: SCAR
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Depositors Institution: Fred Hutchinson Cancer Research Center
Host Species: mouse
Depositors Notes: Fixation for immunostaining: 4% paraformaldehyde in PBS or standard embryo protocol.
Positive Tested Species Reactivity: Drosophila melanogaster
Gene: SCAR
Antigen Molecular Weight: Multiple isoforms. Predicted: 9 and 67-74 kDa; Apparent: 85-90 kDa
Alternate Gene Names: CG4636, Wave, DWave, l(2)k03107
Immunogen: Recombinant SCAR/GST fusion protein
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Uniprot ID: Q9VKM2 
Immunogen Sequence: Full length protein
Entrez Gene ID: 34519 
Additional Information: SCAR: suppressor of cAR. SCAr is a Wiskott-Aldrich Syndrome family protein. SCAR is an actin nucleating factor that functions in lamellipodia formation. SCAR has been used as a marker for lamellipodia. SCAR is the main activator of Arp2/3 in fly embryos.
Antibody Registry ID: AB_2618386 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1C1-SCAR was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P1C1-SCAR)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    IF References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology.
    Gonsalvez GB
    Biology open 4.11 (2015 Oct 12): 1448-61.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

    WB References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

    All References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology.
    Gonsalvez GB
    Biology open 4.11 (2015 Oct 12): 1448-61.

    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization.
    Mohan RD
    eLife 8. (2019 Jul 26): .

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