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SKU: PY654-B-catenin
View product citations for antibody PY654-B-catenin on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: catenin, beta- (MIgM)
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Balsamo, J. / Lilien, J.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Mouse, Xenopus
Depositors Notes:
Antigen Molecular Weight: Predicted: 85 kDa
Human Protein Atlas:  
Predicted Species Reactivity: Human
Gene: Ctnnb1
Immunogen: syntetic phospho-peptide
Alternate Gene Names: Bfc; Mesc; Catnb
Alternate Antibody Name: PY654
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: NH2-CELLHSRNEGVATpYAA-OH
Uniprot ID: Q02248 
Immunogen Sequence: NH2-CELLHSRNEGVATpYAA-OH
Entrez Gene ID: 12387 
Additional Characterization:  
Antibody Registry ID: AB_10144656 
Additional Information: This antibody is specific to beta-catenin phosphorylated at Tyr 654. Beta-1 Catenin is a Key downstream component of the canonical Wnt signaling pathway whose function is affected by differential phosphorylation. Specificity tested on Y654F beta-catenin. See mAb PY489-B-catenin for an antibody specific to beta-catenin phosphorylated at Tyr 489. The catenin target of PY489-B-catenin has been described as nuclear-bound, and the target of PY654-B-catenin has been described as membrane-bound/cytoplasmic [PMID 25367124].
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PY654-B-catenin was deposited to the DSHB by Balsamo, J. / Lilien, J. (DSHB Hybridoma Product PY654-B-catenin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
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