PY489-B-catenin
Catalog Fields
Antigen: catenin, beta-
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Balsamo, J. / Lilien, J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Chicken, Mouse
Depositors Institution: University of Iowa
Antigen Molecular Weight: Predicted: 85kDa
Depositors Notes: Fusion: 09/05.
Immunogen: synthetic phospho-peptide
Gene: Ctnnb1
Alternate Antibody Name: PY489
Alternate Gene Names: Bfc; Mesc; Catnb
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: Q02248
Epitope Location or Sequence: NH2-CRHQEAEMAQNAVRLHpYGL-OH
Entrez Gene ID: 12387
Immunogen Sequence: NH2-CRHQEAEMAQNAVRLHpYGL-OH
Antibody Registry ID: AB_10144551
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: This antibody is specific to beta-catenin phosphorylated at Tyr 489. Beta-1 Catenin is a Key downstream component of the canonical Wnt signaling pathway whose function is affected by differential phosphorylation. Specificity tested on Y489F beta-catenin. See mAb PY654-B-catenin (IgG) for an antibody specific to beta-catenin phosphorylated at Tyr 654. The catenin target of PY489-B-catenin has been described as nuclear-bound/active beta-catenin, and the target of PY654-B-catenin (IgG) has been described as membrane-bound/cytoplasmic beta-catenin.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PY489-B-catenin was deposited to the DSHB by Balsamo, J. / Lilien, J. (DSHB Hybridoma Product PY489-B-catenin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Cables links Robo-bound Abl kinase to N-cadherin-bound beta-catenin to mediate Slit-induced modulation of adhesion and transcription.
Balsamo J
Nature cell biology 9.8 (2007 Aug): 883-92.