PY489-B-catenin

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$40.00
SKU: PY489-B-catenin
View product citations for antibody PY489-B-catenin on CiteAb

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Available: 195

DSHB Data Sheet

Catalog Fields

Antigen: catenin, beta-
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Balsamo, J. / Lilien, J.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Mouse
Depositors Notes: Fusion: 09/05.
Antigen Molecular Weight: Predicted: 85kDa
Human Protein Atlas:
Predicted Species Reactivity: Human
Gene: Ctnnb1
Immunogen: syntetic phospho-peptide
Alternate Gene Names: Bfc; Mesc; Catnb
Alternate Antibody Name: PY489
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: NH2-CRHQEAEMAQNAVRLHpYGL-OH
Uniprot ID: Q02248 
Immunogen Sequence: NH2-CRHQEAEMAQNAVRLHpYGL-OH
Entrez Gene ID: 12387 
Additional Characterization:
Antibody Registry ID: AB_10144551 
Additional Information: This antibody is specific to beta-catenin phosphorylated at Tyr 489. Beta-1 Catenin is a Key downstream component of the canonical Wnt signaling pathway whose function is affected by differential phosphorylation. Specificity tested on Y489F beta-catenin. See mAb PY654-B-catenin (IgG) for an antibody specific to beta-catenin phosphorylated at Tyr 654. The catenin target of PY489-B-catenin has been described as nuclear-bound/active beta-catenin, and the target of PY654-B-catenin (IgG) has been described as membrane-bound/cytoplasmic beta-catenin.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PY489-B-catenin was deposited to the DSHB by Balsamo, J. / Lilien, J. (DSHB Hybridoma Product PY489-B-catenin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Nature cell biology
    Balsamo J
    Nature cell biology 9.8 (2007 Aug): 883-92.

    IF References

    Nature cell biology
    Balsamo J
    Nature cell biology 9.8 (2007 Aug): 883-92.

    Cell research
    Chien KR
    Cell research 24.12 (2014 Dec): 1420-32.

    Genesis (New York, N.Y. : 2000)
    de la Pompa JL
    Genesis (New York, N.Y. : 2000) 51.1 (2013 Jan): 32-40.

    Development (Cambridge, England)
    Zhou CJ
    Development (Cambridge, England) 136.18 (2009 Sep): 3161-71.

    Development (Cambridge, England)
    Yoon JK
    Development (Cambridge, England) 139.10 (2012 May): 1821-30.

    Cell cycle (Georgetown, Tex.)
    Chen ZY
    Cell cycle (Georgetown, Tex.) 10.2 (2011 Jan 15): 337-51.

    The Journal of biological chemistry
    Kim Y
    The Journal of biological chemistry 287.49 (2012 Nov 30): 41103-17.

    WB References

    Nature cell biology
    Balsamo J
    Nature cell biology 9.8 (2007 Aug): 883-92.

    The Journal of biological chemistry
    Kim Y
    The Journal of biological chemistry 287.49 (2012 Nov 30): 41103-17.

    IHC References

    PLoS genetics
    Bagchi IC
    PLoS genetics 8.2 (2012): e1002500.

    Epitope Map References

    Nature cell biology
    Balsamo J
    Nature cell biology 9.8 (2007 Aug): 883-92.

    All References

    PLoS genetics
    Bagchi IC
    PLoS genetics 8.2 (2012): e1002500.

    Nature cell biology
    Balsamo J
    Nature cell biology 9.8 (2007 Aug): 883-92.

    Cell research
    Chien KR
    Cell research 24.12 (2014 Dec): 1420-32.

    Genesis (New York, N.Y. : 2000)
    de la Pompa JL
    Genesis (New York, N.Y. : 2000) 51.1 (2013 Jan): 32-40.

    Development (Cambridge, England)
    Zhou CJ
    Development (Cambridge, England) 136.18 (2009 Sep): 3161-71.

    Development (Cambridge, England)
    Yoon JK
    Development (Cambridge, England) 139.10 (2012 May): 1821-30.

    Cell cycle (Georgetown, Tex.)
    Chen ZY
    Cell cycle (Georgetown, Tex.) 10.2 (2011 Jan 15): 337-51.

    The Journal of biological chemistry
    Kim Y
    The Journal of biological chemistry 287.49 (2012 Nov 30): 41103-17.

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