bTAN 20
Catalog Fields
Clone ID/Product Name: bTAN 20
Available to For-Profits: Yes
Alternate Antibody Name: 15A
Gene Symbol: NOTCH1
Ab Isotype: RIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2153497
Uniprot ID: P46531
Entrez Gene ID: 4851
Clonality: Monoclonal
Immunogen: Recombinant fusion protein GST-Notch1 intrcellular domain
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63Ag8.653 (ATCC CRL-1580)
Epitope Mapped: Yes
Antigen Name: Notch1 intracellular domain (human)
Epitope Location or Sequence: intracellular domain
Alternate Antigen Name:
Deposit Date: 5/2/2000
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110-120 kDa
Depositor: Artavanis-Tsakonas, S.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Human
Depositor Notes: bTAN 20 doesn't work well for immunoprecipitation
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Human, Mouse
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bTAN 20 was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product bTAN 20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Alterations in Notch signaling in neoplastic lesions of the human cervix.
Artavanis-Tsakonas S
Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.