bTAN 20

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$45.00 to $425.00
SKU: bTAN 20
View product citations for antibody bTAN 20 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Notch1 intracellular domain (human)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG1, kappa light chain
Depositor: Artavanis-Tsakonas, S.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Institution: Harvard Medical School
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110-120 kDa
Depositors Notes: bTAN 20 doesn't work well for immunoprecipitation
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant fusion protein GST-Notch1 intrcellular domain
Gene: NOTCH1
Alternate Antibody Name: 15A
Alternate Gene Names: hN1, AOS5, TAN1, AOVD1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653 (ATCC CRL-1580)
Epitope Mapped: Yes
Uniprot ID: P46531 
Epitope Location or Sequence: intracellular domain
Entrez Gene ID: 4851 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2153497 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bTAN 20 was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product bTAN 20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    IF References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    WB References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.
    Artavanis-Tsakonas S
    Cell 90.2 (1997 Jul 25): 281-91.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Sarin A
    The Journal of biological chemistry 279.4 (2004 Jan 23): 2937-44.

    Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1.
    Bertrand FE
    Cell cycle (Georgetown, Tex.) 4.10 (2005 Oct): 1389-95.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    IHC References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Expression of Notch in a case of osteosarcoma of the maxilla.
    Nagai N
    European journal of medical research 9.11 (2004 Nov 29): 533-5.

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
    Kawakami T
    The Angle orthodontist 75.6 (2005 Nov): 993-5.

    Notch signaling in mandibular condylar cartilage development.
    Kawakami T
    European journal of medical research 12.10 (2007 Oct 30): 515-9.

    Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study).
    Kiaris H
    The open dentistry journal 4. (2010 Jul 27): 153-8.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    Epitope Map References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    All References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Expression of Notch in a case of osteosarcoma of the maxilla.
    Nagai N
    European journal of medical research 9.11 (2004 Nov 29): 533-5.

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
    Kawakami T
    The Angle orthodontist 75.6 (2005 Nov): 993-5.

    Notch signaling in mandibular condylar cartilage development.
    Kawakami T
    European journal of medical research 12.10 (2007 Oct 30): 515-9.

    Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study).
    Kiaris H
    The open dentistry journal 4. (2010 Jul 27): 153-8.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.
    Artavanis-Tsakonas S
    Cell 90.2 (1997 Jul 25): 281-91.

    The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Sarin A
    The Journal of biological chemistry 279.4 (2004 Jan 23): 2937-44.

    Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1.
    Bertrand FE
    Cell cycle (Georgetown, Tex.) 4.10 (2005 Oct): 1389-95.

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