bTAN 20
Catalog Fields
Antigen: Notch1 intracellular domain (human)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG1, kappa light chain
Depositor: Artavanis-Tsakonas, S.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Institution: Harvard Medical School
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110-120 kDa
Depositors Notes: bTAN 20 doesn't work well for immunoprecipitation
Immunogen: Recombinant fusion protein GST-Notch1 intrcellular domain
Gene: NOTCH1
Alternate Antibody Name: 15A
Alternate Gene Names: hN1, AOS5, TAN1, AOVD1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653 (ATCC CRL-1580)
Epitope Mapped: Yes
Uniprot ID: P46531
Epitope Location or Sequence: intracellular domain
Entrez Gene ID: 4851
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2153497
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bTAN 20 was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product bTAN 20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Alterations in Notch signaling in neoplastic lesions of the human cervix.
Artavanis-Tsakonas S
Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.