bTAN 20

(0) No Reviews yet
$40.00
SKU: bTAN 20
View product citations for antibody bTAN 20 on CiteAb

In Stock

Available: 325

DSHB Data Sheet

Catalog Fields

Antigen: Notch1 intracellular domain (human)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Artavanis-Tsakonas, S.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: bTAN 20 doesn't work well for immunoprecipitation
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110-120 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NOTCH1
Immunogen: Recombinant fusion protein GST-Notch1 intrcellular domain
Alternate Gene Names: hN1, AOS5, TAN1, AOVD1
Alternate Antibody Name: 15A
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653 (ATCC CRL-1580)
Epitope Location or Sequence: intracellular domain
Uniprot ID: P46531 
Immunogen Sequence: Partial protein
Entrez Gene ID: 4851 
Additional Characterization:
Antibody Registry ID: AB_2153497 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bTAN 20 was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product bTAN 20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    IF References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    WB References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.
    Artavanis-Tsakonas S
    Cell 90.2 (1997 Jul 25): 281-91.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Sarin A
    The Journal of biological chemistry 279.4 (2004 Jan 23): 2937-44.

    Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1.
    Bertrand FE
    Cell cycle (Georgetown, Tex.) 4.10 (2005 Oct): 1389-95.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    IHC References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Expression of Notch in a case of osteosarcoma of the maxilla.
    Nagai N
    European journal of medical research 9.11 (2004 Nov 29): 533-5.

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
    Kawakami T
    The Angle orthodontist 75.6 (2005 Nov): 993-5.

    Notch signaling in mandibular condylar cartilage development.
    Kawakami T
    European journal of medical research 12.10 (2007 Oct 30): 515-9.

    Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study).
    Kiaris H
    The open dentistry journal 4. (2010 Jul 27): 153-8.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    Epitope Map References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    All References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Expression of Notch in a case of osteosarcoma of the maxilla.
    Nagai N
    European journal of medical research 9.11 (2004 Nov 29): 533-5.

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
    Kawakami T
    The Angle orthodontist 75.6 (2005 Nov): 993-5.

    Notch signaling in mandibular condylar cartilage development.
    Kawakami T
    European journal of medical research 12.10 (2007 Oct 30): 515-9.

    Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study).
    Kiaris H
    The open dentistry journal 4. (2010 Jul 27): 153-8.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation.
    Andrews PW
    Stem cells (Dayton, Ohio) 26.3 (2008 Mar): 715-23.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.
    Artavanis-Tsakonas S
    Cell 90.2 (1997 Jul 25): 281-91.

    The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Sarin A
    The Journal of biological chemistry 279.4 (2004 Jan 23): 2937-44.

    Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1.
    Bertrand FE
    Cell cycle (Georgetown, Tex.) 4.10 (2005 Oct): 1389-95.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review