G8.8

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SKU: G8.8

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: G8.8
Available to For-Profits: Yes
Alternate Antibody Name: CD326
Gene Symbol: Epcam
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2098655 
Uniprot ID: Q99JW5 
RRID:  
Entrez Gene ID: 17075 
Clonality: Monoclonal
Immunogen: TE-71 mouse thymic epithelial cell line
Clone:
Immunogen Sequence: Full length native protein
Myeloma Strain: X63.Ag8.653
Epitope Mapped: No
Antigen Name: Epithelial cell adhesion molecule (murine); CD326
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/11/1997
Antigen Molecular Weight: ~40 kDa
Depositor: Farr, A.G .
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Mouse
Depositor Notes: This antibody does not recognize human or rat EpCAM.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: G8.8 has also been used to purify thymic epithelial cells (PMID: 20548034 17127749). RRID:AB_2098655
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G8.8 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product G8.8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

39 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FACS References
  • All References
    • Initial Publication

    Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.5 (1991 May): 645-53.

    IF References

    microRNA-449a modulates medullary thymic epithelial cell differentiation.
    Zhang X
    Scientific reports 7.1 (2017 Nov 21): 15915.

    Hippo signaling interactions with Wnt/β-catenin and Notch signaling repress liver tumorigenesis.
    Yang Y
    The Journal of clinical investigation 127.1 (2017 Jan 3): 137-152.

    Control of inflammation by stromal Hedgehog pathway activation restrains colitis.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 113.47 (2016 Nov 22): E7545-E7553.

    Self-renewing diploid Axin2(+) cells fuel homeostatic renewal of the liver.
    Nusse R
    Nature 524.7564 (2015 Aug 13): 180-5.

    Premature thymic involution is independent of structural plasticity of the thymic stroma.
    Liston A
    European journal of immunology 45.5 (2015 May): 1535-47.

    Hedgehog signaling restrains bladder cancer progression by eliciting stromal production of urothelial differentiation factors.
    Beachy PA
    Cancer cell 26.4 (2014 Oct 13): 521-33.

    Stromal response to Hedgehog signaling restrains pancreatic cancer progression.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 111.30 (2014 Jul 29): E3091-100.

    Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
    Alexandropoulos K
    PloS one 9.1 (2014): e86129.

    Established thymic epithelial progenitor/stem cell-like cell lines differentiate into mature thymic epithelial cells and support T cell development.
    Zhang X
    PloS one 8.9 (2013): e75222.

    Comparison of mAbs targeting epithelial cell adhesion molecule for the detection of prostate cancer lymph node metastases with multimodal contrast agents: quantitative small-animal PET/CT and NIRF.
    Sevick-Muraca EM
    Journal of nuclear medicine : official publication, Society of Nuclear Medicine 53.9 (2012 Sep): 1427-37.

    Wnt signaling specifies and patterns intestinal endoderm.
    Melton DA
    Mechanisms of development 128.7-10 (2011 Sep-Dec): 387-400.

    Abnormal placental development and early embryonic lethality in EpCAM-null mice.
    Udey MC
    PloS one 4.12 (2009 Dec 31): e8543.

    Transcriptional dynamics of endodermal organ formation.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 29-42.

    Alterations of the medullary epithelial compartment in the Aire-deficient thymus: implications for programs of thymic epithelial differentiation.
    Farr AG
    Journal of immunology (Baltimore, Md. : 1950) 181.8 (2008 Oct 15): 5225-32.

    Therapeutic window of MuS110, a single-chain antibody construct bispecific for murine EpCAM and murine CD3.
    Schlereth B
    Cancer research 68.1 (2008 Jan 1): 143-51.

    Prospective isolation and global gene expression analysis of definitive and visceral endoderm.
    Melton DA
    Developmental biology 304.2 (2007 Apr 15): 541-55.

    Genetic and epigenetic properties of mouse male germline stem cells during long-term culture.
    Shinohara T
    Development (Cambridge, England) 132.18 (2005 Sep): 4155-63.

    Keratin-8-deficient mice develop chronic spontaneous Th2 colitis amenable to antibiotic treatment.
    Omary MB
    Journal of cell science 118.Pt 9 (2005 May 1): 1971-80.

    Vaginal submucosal dendritic cells, but not Langerhans cells, induce protective Th1 responses to herpes simplex virus-2.
    Iwasaki A
    The Journal of experimental medicine 197.2 (2003 Jan 20): 153-62.

    Murine spermatogonial stem cells: targeted transgene expression and purification in an active state.
    Cuzin F
    EMBO reports 3.8 (2002 Aug): 753-9.

    WB References

    The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells.
    Farr AG
    European journal of immunology 26.2 (1996 Feb): 401-8.

    IHC References

    Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.5 (1991 May): 645-53.

    Notch signaling controls liver development by regulating biliary differentiation.
    Stanger BZ
    Development (Cambridge, England) 136.10 (2009 May): 1727-39.

    Expression of nestin-green fluorescent protein transgene marks oval cells in the adult liver.
    Enikolopov G
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 413-21.

    The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells.
    Farr AG
    European journal of immunology 26.2 (1996 Feb): 401-8.

    Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells.
    Udey MC
    European journal of immunology 26.1 (1996 Jan): 110-4.

    ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion.
    Chandler RL
    Nature communications 10.1 (2019 Aug 7): 3554.

    IP References

    Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.5 (1991 May): 645-53.

    FFPE References

    ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion.
    Chandler RL
    Nature communications 10.1 (2019 Aug 7): 3554.

    FACS References

    Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.5 (1991 May): 645-53.

    Dynamic spatio-temporal contribution of single β5t+ cortical epithelial precursors to the thymus medulla.
    Holländer GA
    European journal of immunology 46.4 (2016 Apr): 846-56.

    NLRC5 exclusively transactivates MHC class I and related genes through a distinctive SXY module.
    Guarda G
    PLoS genetics 11.3 (2015 Mar): e1005088.

    Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
    Alexandropoulos K
    PloS one 9.1 (2014): e86129.

    MicroRNAs control the maintenance of thymic epithelia and their competence for T lineage commitment and thymocyte selection.
    Holländer GA
    Journal of immunology (Baltimore, Md. : 1950) 189.8 (2012 Oct 15): 3894-904.

    Foxn1 regulates lineage progression in cortical and medullary thymic epithelial cells but is dispensable for medullary sublineage divergence.
    Blackburn CC
    PLoS genetics 7.11 (2011 Nov): e1002348.

    Commensal-dependent expression of IL-25 regulates the IL-23-IL-17 axis in the intestine.
    Artis D
    The Journal of experimental medicine 205.10 (2008 Sep 29): 2191-8.

    Akt mediates self-renewal division of mouse spermatogonial stem cells.
    Shinohara T
    Development (Cambridge, England) 134.10 (2007 May): 1853-9.

    Leukemia inhibitory factor enhances formation of germ cell colonies in neonatal mouse testis culture.
    Shinohara T
    Biology of reproduction 76.1 (2007 Jan): 55-62.

    The Wnt signaling antagonist Kremen1 is required for development of thymic architecture.
    Pezzano M
    Clinical & developmental immunology 13.2-4 (2006 Jun-Dec): 299-319.

    Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
    Polychronakos C
    Diabetes 55.9 (2006 Sep): 2595-601.

    Genetic and epigenetic properties of mouse male germline stem cells during long-term culture.
    Shinohara T
    Development (Cambridge, England) 132.18 (2005 Sep): 4155-63.

    Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions.
    Shinohara T
    Biology of reproduction 72.4 (2005 Apr): 985-91.

    Generation of pluripotent stem cells from neonatal mouse testis.
    Shinohara T
    Cell 119.7 (2004 Dec 29): 1001-12.

    Identification and enrichment of spermatogonial stem cells displaying side-population phenotype in immature mouse testis.
    Vicini E
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18.2 (2004 Feb): 376-8.

    Long-term proliferation in culture and germline transmission of mouse male germline stem cells.
    Shinohara T
    Biology of reproduction 69.2 (2003 Aug): 612-6.

    The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells.
    Farr AG
    European journal of immunology 26.2 (1996 Feb): 401-8.

    Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells.
    Udey MC
    European journal of immunology 26.1 (1996 Jan): 110-4.

    All References

    Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.5 (1991 May): 645-53.

    Notch signaling controls liver development by regulating biliary differentiation.
    Stanger BZ
    Development (Cambridge, England) 136.10 (2009 May): 1727-39.

    Expression of nestin-green fluorescent protein transgene marks oval cells in the adult liver.
    Enikolopov G
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 413-21.

    The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells.
    Farr AG
    European journal of immunology 26.2 (1996 Feb): 401-8.

    Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells.
    Udey MC
    European journal of immunology 26.1 (1996 Jan): 110-4.

    ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion.
    Chandler RL
    Nature communications 10.1 (2019 Aug 7): 3554.

    microRNA-449a modulates medullary thymic epithelial cell differentiation.
    Zhang X
    Scientific reports 7.1 (2017 Nov 21): 15915.

    Hippo signaling interactions with Wnt/β-catenin and Notch signaling repress liver tumorigenesis.
    Yang Y
    The Journal of clinical investigation 127.1 (2017 Jan 3): 137-152.

    Control of inflammation by stromal Hedgehog pathway activation restrains colitis.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 113.47 (2016 Nov 22): E7545-E7553.

    Self-renewing diploid Axin2(+) cells fuel homeostatic renewal of the liver.
    Nusse R
    Nature 524.7564 (2015 Aug 13): 180-5.

    Premature thymic involution is independent of structural plasticity of the thymic stroma.
    Liston A
    European journal of immunology 45.5 (2015 May): 1535-47.

    Hedgehog signaling restrains bladder cancer progression by eliciting stromal production of urothelial differentiation factors.
    Beachy PA
    Cancer cell 26.4 (2014 Oct 13): 521-33.

    Stromal response to Hedgehog signaling restrains pancreatic cancer progression.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 111.30 (2014 Jul 29): E3091-100.

    Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
    Alexandropoulos K
    PloS one 9.1 (2014): e86129.

    Established thymic epithelial progenitor/stem cell-like cell lines differentiate into mature thymic epithelial cells and support T cell development.
    Zhang X
    PloS one 8.9 (2013): e75222.

    Comparison of mAbs targeting epithelial cell adhesion molecule for the detection of prostate cancer lymph node metastases with multimodal contrast agents: quantitative small-animal PET/CT and NIRF.
    Sevick-Muraca EM
    Journal of nuclear medicine : official publication, Society of Nuclear Medicine 53.9 (2012 Sep): 1427-37.

    Wnt signaling specifies and patterns intestinal endoderm.
    Melton DA
    Mechanisms of development 128.7-10 (2011 Sep-Dec): 387-400.

    Abnormal placental development and early embryonic lethality in EpCAM-null mice.
    Udey MC
    PloS one 4.12 (2009 Dec 31): e8543.

    Transcriptional dynamics of endodermal organ formation.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 29-42.

    Alterations of the medullary epithelial compartment in the Aire-deficient thymus: implications for programs of thymic epithelial differentiation.
    Farr AG
    Journal of immunology (Baltimore, Md. : 1950) 181.8 (2008 Oct 15): 5225-32.

    Therapeutic window of MuS110, a single-chain antibody construct bispecific for murine EpCAM and murine CD3.
    Schlereth B
    Cancer research 68.1 (2008 Jan 1): 143-51.

    Prospective isolation and global gene expression analysis of definitive and visceral endoderm.
    Melton DA
    Developmental biology 304.2 (2007 Apr 15): 541-55.

    Genetic and epigenetic properties of mouse male germline stem cells during long-term culture.
    Shinohara T
    Development (Cambridge, England) 132.18 (2005 Sep): 4155-63.

    Keratin-8-deficient mice develop chronic spontaneous Th2 colitis amenable to antibiotic treatment.
    Omary MB
    Journal of cell science 118.Pt 9 (2005 May 1): 1971-80.

    Vaginal submucosal dendritic cells, but not Langerhans cells, induce protective Th1 responses to herpes simplex virus-2.
    Iwasaki A
    The Journal of experimental medicine 197.2 (2003 Jan 20): 153-62.

    Murine spermatogonial stem cells: targeted transgene expression and purification in an active state.
    Cuzin F
    EMBO reports 3.8 (2002 Aug): 753-9.

    Dynamic spatio-temporal contribution of single β5t+ cortical epithelial precursors to the thymus medulla.
    Holländer GA
    European journal of immunology 46.4 (2016 Apr): 846-56.

    NLRC5 exclusively transactivates MHC class I and related genes through a distinctive SXY module.
    Guarda G
    PLoS genetics 11.3 (2015 Mar): e1005088.

    MicroRNAs control the maintenance of thymic epithelia and their competence for T lineage commitment and thymocyte selection.
    Holländer GA
    Journal of immunology (Baltimore, Md. : 1950) 189.8 (2012 Oct 15): 3894-904.

    Foxn1 regulates lineage progression in cortical and medullary thymic epithelial cells but is dispensable for medullary sublineage divergence.
    Blackburn CC
    PLoS genetics 7.11 (2011 Nov): e1002348.

    Commensal-dependent expression of IL-25 regulates the IL-23-IL-17 axis in the intestine.
    Artis D
    The Journal of experimental medicine 205.10 (2008 Sep 29): 2191-8.

    Akt mediates self-renewal division of mouse spermatogonial stem cells.
    Shinohara T
    Development (Cambridge, England) 134.10 (2007 May): 1853-9.

    Leukemia inhibitory factor enhances formation of germ cell colonies in neonatal mouse testis culture.
    Shinohara T
    Biology of reproduction 76.1 (2007 Jan): 55-62.

    The Wnt signaling antagonist Kremen1 is required for development of thymic architecture.
    Pezzano M
    Clinical & developmental immunology 13.2-4 (2006 Jun-Dec): 299-319.

    Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
    Polychronakos C
    Diabetes 55.9 (2006 Sep): 2595-601.

    Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions.
    Shinohara T
    Biology of reproduction 72.4 (2005 Apr): 985-91.

    Generation of pluripotent stem cells from neonatal mouse testis.
    Shinohara T
    Cell 119.7 (2004 Dec 29): 1001-12.

    Identification and enrichment of spermatogonial stem cells displaying side-population phenotype in immature mouse testis.
    Vicini E
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18.2 (2004 Feb): 376-8.

    Long-term proliferation in culture and germline transmission of mouse male germline stem cells.
    Shinohara T
    Biology of reproduction 69.2 (2003 Aug): 612-6.

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