Antigen: Cubitus interruptus (Ci) full length
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Holmgren, R.
Host Species: rat
Depositors Institution: Northwestern University
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: 155 kDa Apparent:180 kDa,
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Drosophila protein expressed in E. coli and isolated from inclusion bodies.
Alternate Gene Names: Ci; ci-D; Ci/Gli; Ci155; ciD; Gli; Siah
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO-1
Epitope Location or Sequence: a.a. 700-850 at the C-terminus of the Zn finger domain
Uniprot ID: P19538
Entrez Gene ID: 43767
Antibody Registry ID: AB_2109711
Additional Information: Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A1 was deposited to the DSHB by Holmgren, R. (DSHB Hybridoma Product 2A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References