2A1

(0) No Reviews yet
$40.00
SKU: 2A1
View product citations for antibody 2A1 on CiteAb

In Stock

Available: 167

DSHB Data Sheet

Catalog Fields

Antigen: Cubitus interruptus (Ci) full length
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Holmgren, R.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Northwestern University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 155 kDa Apparent:180 kDa,
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CI
Immunogen: Drosophila protein expressed in E. coli and isolated from inclusion bodies.
Alternate Gene Names: Ci; ci-D; Ci/Gli; Ci155; ciD; Gli; Siah
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO-1
Epitope Location or Sequence: a.a. 700-850 at the C-terminus of the Zn finger domain
Uniprot ID: P19538 
Immunogen Sequence:
Entrez Gene ID: 43767 
Additional Characterization:  
Antibody Registry ID: AB_2109711 
Additional Information: Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A1 was deposited to the DSHB by Holmgren, R. (DSHB Hybridoma Product 2A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Drosophila heparan sulfate 3-O sulfotransferase B null mutant is viable and exhibits no defects in Notch signaling.
    Lin X
    Journal of genetics and genomics = Yi chuan xue bao 41.7 (2014 Jul 20): 369-78.

    Transgressions of compartment boundaries and cell reprogramming during regeneration in Drosophila.
    Morata G
    eLife 3. (2014 Apr 22): e01831.

    Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.
    Zhu AJ
    Development (Cambridge, England) 141.7 (2014 Apr): 1473-9.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Importin α1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal.
    Zhang L
    The Journal of biological chemistry 291.15 (2016 Apr 8): 7926-37.

    Quantitative Morphological Variation in the Developing <i>Drosophila</i> Wing.
    Houle D
    G3 (Bethesda, Md.) 8.7 (2018 Jul 2): 2399-2409.

    HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.
    Zhou Z
    Biochemical and biophysical research communications 513.4 (2019 Jun 11): 1013-1018.

    WB References
    IHC References

    The Drosophila cubitus interruptus protein and its role in the wingless and hedgehog signal transduction pathways.
    Holmgren R
    Mechanisms of development 52.1 (1995 Jul): 137-50.

    Proteolysis that is inhibited by hedgehog targets Cubitus interruptus protein to the nucleus and converts it to a repressor.
    Kornberg TB
    Cell 89.7 (1997 Jun 27): 1043-53.

    Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.
    Basler K
    Cell 96.6 (1999 Mar 19): 819-31.

    Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal.
    Ishii S
    PloS one 5.12 (2010 Dec 15): e15365.

    Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator.
    Kalderon D
    Nature 396.6713 (1998 Dec 24-31): 749-53.

    Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues.
    Milán M
    Cell cycle (Georgetown, Tex.) 13.9 (2014): 1450-5.

    Disruption of Drosophila melanogaster lipid metabolism genes causes tissue overgrowth associated with altered developmental signaling.
    Fortini ME
    PLoS genetics 9.11 (2013 Nov): e1003917.

    Riquiqui and minibrain are regulators of the hippo pathway downstream of Dachsous.
    Harvey KF
    Nature cell biology 15.10 (2013 Oct): 1176-85.

    Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
    Beachy PA
    Molecular cell 12.5 (2003 Nov): 1261-74.

    Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase.
    Duronio RJ
    Developmental cell 2.6 (2002 Jun): 757-70.

    Regulation of Hedgehog signaling: a complex story.
    Robbins DJ
    Biochemical pharmacology 67.5 (2004 Mar 1): 805-14.

    Phosphorylation of the atypical kinesin Costal2 by the kinase Fused induces the partial disassembly of the Smoothened-Fused-Costal2-Cubitus interruptus complex in Hedgehog signalling.
    Thérond PP
    Development (Cambridge, England) 134.20 (2007 Oct): 3677-89.

    CSN-mediated deneddylation differentially modulates Ci(155) proteolysis to promote Hedgehog signalling responses.
    Chien CT
    Nature communications 2. (2011 Feb 8): 182.

    Smoothened translates Hedgehog levels into distinct responses.
    Hooper JE
    Development (Cambridge, England) 130.17 (2003 Sep): 3951-63.

    Differential range and activity of various forms of the Hedgehog protein.
    van den Heuvel M
    BMC developmental biology 5. (2005 Sep 30): 21.

    A PI4KIIIα protein complex is required for cell viability during Drosophila wing development.
    Raghu P
    Developmental biology 462.2 (2020 Jun 15): 208-222.

    IP References
    Epitope Map References
    All References

    The Drosophila cubitus interruptus protein and its role in the wingless and hedgehog signal transduction pathways.
    Holmgren R
    Mechanisms of development 52.1 (1995 Jul): 137-50.

    Proteolysis that is inhibited by hedgehog targets Cubitus interruptus protein to the nucleus and converts it to a repressor.
    Kornberg TB
    Cell 89.7 (1997 Jun 27): 1043-53.

    Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.
    Basler K
    Cell 96.6 (1999 Mar 19): 819-31.

    Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal.
    Ishii S
    PloS one 5.12 (2010 Dec 15): e15365.

    Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator.
    Kalderon D
    Nature 396.6713 (1998 Dec 24-31): 749-53.

    Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues.
    Milán M
    Cell cycle (Georgetown, Tex.) 13.9 (2014): 1450-5.

    Disruption of Drosophila melanogaster lipid metabolism genes causes tissue overgrowth associated with altered developmental signaling.
    Fortini ME
    PLoS genetics 9.11 (2013 Nov): e1003917.

    Riquiqui and minibrain are regulators of the hippo pathway downstream of Dachsous.
    Harvey KF
    Nature cell biology 15.10 (2013 Oct): 1176-85.

    Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
    Beachy PA
    Molecular cell 12.5 (2003 Nov): 1261-74.

    Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase.
    Duronio RJ
    Developmental cell 2.6 (2002 Jun): 757-70.

    Regulation of Hedgehog signaling: a complex story.
    Robbins DJ
    Biochemical pharmacology 67.5 (2004 Mar 1): 805-14.

    Phosphorylation of the atypical kinesin Costal2 by the kinase Fused induces the partial disassembly of the Smoothened-Fused-Costal2-Cubitus interruptus complex in Hedgehog signalling.
    Thérond PP
    Development (Cambridge, England) 134.20 (2007 Oct): 3677-89.

    CSN-mediated deneddylation differentially modulates Ci(155) proteolysis to promote Hedgehog signalling responses.
    Chien CT
    Nature communications 2. (2011 Feb 8): 182.

    Smoothened translates Hedgehog levels into distinct responses.
    Hooper JE
    Development (Cambridge, England) 130.17 (2003 Sep): 3951-63.

    Differential range and activity of various forms of the Hedgehog protein.
    van den Heuvel M
    BMC developmental biology 5. (2005 Sep 30): 21.

    A PI4KIIIα protein complex is required for cell viability during Drosophila wing development.
    Raghu P
    Developmental biology 462.2 (2020 Jun 15): 208-222.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Drosophila heparan sulfate 3-O sulfotransferase B null mutant is viable and exhibits no defects in Notch signaling.
    Lin X
    Journal of genetics and genomics = Yi chuan xue bao 41.7 (2014 Jul 20): 369-78.

    Transgressions of compartment boundaries and cell reprogramming during regeneration in Drosophila.
    Morata G
    eLife 3. (2014 Apr 22): e01831.

    Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.
    Zhu AJ
    Development (Cambridge, England) 141.7 (2014 Apr): 1473-9.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Importin α1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal.
    Zhang L
    The Journal of biological chemistry 291.15 (2016 Apr 8): 7926-37.

    Quantitative Morphological Variation in the Developing <i>Drosophila</i> Wing.
    Houle D
    G3 (Bethesda, Md.) 8.7 (2018 Jul 2): 2399-2409.

    HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.
    Zhou Z
    Biochemical and biophysical research communications 513.4 (2019 Jun 11): 1013-1018.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review