2A1
Catalog Fields
Product Name/ID: 2A1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CI
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID: AB_2109711
Uniprot ID: P19538
Entrez Gene ID: 43767
Clonality: Monoclonal
Immunogen: Drosophila protein expressed in E. coli and isolated from inclusion bodies.
Clone:
Immunogen Sequence:
Myeloma Strain: NSO-1
Epitope Mapped: Yes
Antigen Name: Cubitus interruptus (Ci) full length
Epitope Location or Sequence: a.a. 700-850 at the C-terminus of the Zn finger domain
Alternate Antigen Name:
Deposit Date: 9/27/2009
Antigen Molecular Weight: Predicted: 155 kDa Apparent:180 kDa,
Depositor: Holmgren, R.
Antigen Sequence:
Depositor Institution: Northwestern University
Antigen Species: Drosophila
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A1 was deposited to the DSHB by Holmgren, R. (DSHB Hybridoma Product 2A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
32 References
Initial Publication
The Drosophila cubitus interruptus protein and its role in the wingless and hedgehog signal transduction pathways.
Holmgren R
Mechanisms of development 52.1 (1995 Jul): 137-50.