2A1

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  • Immunofluorescent (IF) image of Drosophila wing discs labeled with DSHB antibodies 2A1 against Cubitus interruptus, Ci (blue) and Smoothened (20C6) (red). PMID: 39402333, Fig. EV3C.
  • Immunofluorescent image of a Drosophila wing disc labeled with DSHB antibody 2A1 against Cubitus Interruptus (red). PMID: 37847757, Fig. 1D.
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SKU: 2A1

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Catalog Fields

Clone ID/Product Name: 2A1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CI
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2109711 
Uniprot ID: P19538 
RRID:  
Entrez Gene ID: 43767 
Clonality: Monoclonal
Immunogen: Drosophila protein expressed in E. coli and isolated from inclusion bodies.
Clone:
Immunogen Sequence:
Myeloma Strain: NSO-1
Epitope Mapped: Yes
Antigen Name: Cubitus interruptus (Ci) full length
Epitope Location or Sequence: a.a. 700-850 at the C-terminus of the Zn finger domain
Alternate Antigen Name:
Deposit Date: 9/27/2009
Antigen Molecular Weight: Predicted: 155 kDa Apparent:180 kDa,
Depositor: Holmgren, R.
Antigen Sequence:
Depositor Institution: Northwestern University
Antigen Species: Drosophila
Depositor Notes:
Host Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
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