Drosophila Ptc (Apa 1)
Catalog Fields
Clone ID/Product Name: Drosophila Ptc (Apa 1)
Available to For-Profits: Yes
Alternate Antibody Name: Apa 1.3
Gene Symbol: ptc
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Entrez Gene ID: 35851
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing the first extracellular loop of Patched, aa 106-346.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NP-3
Epitope Mapped: Yes
Antigen Name: Patched (extracellular region)
Epitope Location or Sequence: aa 106-346
Alternate Antigen Name:
Deposit Date: 10/7/2005
Antigen Molecular Weight: Predicted: 143 kDa
Depositor: Guerrero, I.
Antigen Sequence:
Depositor Institution: Universidad Autonoma de Madrid
Antigen Species: Drosophila
Depositor Notes: Drosophila Hedgehog receptor, ptc mutant embryos show a segment polarity phenotype
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila, Lizard
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Drosophila Ptc (Apa 1) was deposited to the DSHB by Guerrero, I. (DSHB Hybridoma Product Drosophila Ptc (Apa 1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
20 References
Initial Publication
Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryos.
Guerrero I
Development (Cambridge, England) 120.4 (1994 Apr): 987-98.