Drosophila Ptc (Apa 1)

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$40.00
SKU: Drosophila Ptc (Apa 1)
View product citations for antibody Drosophila Ptc (Apa 1) on CiteAb

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Available: 114

DSHB Data Sheet

Catalog Fields

Antigen: Patched (extracellular region)
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Guerrero, I.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universidad Autonoma de Madrid
Positive Tested Species Reactivity: Drosophila, Lizard
Depositors Notes: Drosophila Hedgehog receptor, ptc mutant embryos show a segment polarity phenotype
Antigen Molecular Weight: Predicted: 143 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ptc
Immunogen: Recombinant fusion protein containing the first extracellular loop of Patched, aa 106-346.
Alternate Gene Names: CG2411, ptch
Alternate Antibody Name: Apa 1.3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NP-3
Epitope Location or Sequence: aa 106-346
Uniprot ID: P18502 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35851 
Additional Characterization:
Antibody Registry ID: AB_528441 
Additional Information: For experiments involving live cells and tissue, Drosophila Ptc (Apa 1) supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Drosophila Ptc (Apa 1) was deposited to the DSHB by Guerrero, I. (DSHB Hybridoma Product Drosophila Ptc (Apa 1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The Drosophila segment polarity gene patched interacts with decapentaplegic in wing development.
    Guerrero I
    The EMBO journal 13.1 (1994 Jan 1): 71-82.

    Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryos.
    Guerrero I
    Development (Cambridge, England) 120.4 (1994 Apr): 987-98.

    The sterol-sensing domain of Patched protein seems to control Smoothened activity through Patched vesicular trafficking.
    Guerrero I
    Current biology : CB 11.8 (2001 Apr 17): 601-7.

    Patched controls the Hedgehog gradient by endocytosis in a dynamin-dependent manner, but this internalization does not play a major role in signal transduction.
    Guerrero I
    Development (Cambridge, England) 131.10 (2004 May): 2395-408.

    The Drosophila ortholog of the human Wnt inhibitor factor Shifted controls the diffusion of lipid-modified Hedgehog.
    Guerrero I
    Developmental cell 8.2 (2005 Feb): 241-53.

    Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner.
    Jia J
    Developmental biology 366.2 (2012 Jun 15): 172-84.

    Hyperplastic discs differentially regulates the transcriptional outputs of hedgehog signaling.
    Lin X
    Mechanisms of development 133. (2014 Aug): 117-25.

    Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover.
    Chen D
    PLoS biology 11.11 (2013 Nov): e1001721.

    Detecting tagged Hedgehog with intracellular and extracellular immunocytochemistry for functional analysis.
    Guerrero I
    Methods in molecular biology (Clifton, N.J.) 397. (2007): 91-103.

    Non-cell autonomous control of apoptosis by ligand-independent Hedgehog signaling in Drosophila.
    Bergmann A
    Cell death and differentiation 20.2 (2013 Feb): 302-11.

    The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.
    Zhao Y
    The Journal of biological chemistry 289.32 (2014 Aug 8): 22333-41.

    Smoothened oligomerization/higher order clustering in lipid rafts is essential for high Hedgehog activity transduction.
    Zhao Y
    The Journal of biological chemistry 288.18 (2013 May 3): 12605-14.

    Transcription in the absence of histone H3.3.
    Basler K
    Current biology : CB 19.14 (2009 Jul 28): 1221-6.

    Dynamic phosphorylation of the kinesin Costal-2 in vivo reveals requirement of fused kinase activity for all levels of hedgehog signalling.
    Thérond PP
    Developmental biology 344.1 (2010 Aug 1): 119-28.

    Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation.
    O'Reilly AM
    The Journal of cell biology 191.5 (2010 Nov 29): 943-52.

    Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila.
    Jia J
    Methods in molecular biology (Clifton, N.J.) 1322. (2015): 45-60.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    WB References
    IHC References
    Epitope Map References
    All References

    The Drosophila segment polarity gene patched interacts with decapentaplegic in wing development.
    Guerrero I
    The EMBO journal 13.1 (1994 Jan 1): 71-82.

    Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryos.
    Guerrero I
    Development (Cambridge, England) 120.4 (1994 Apr): 987-98.

    Gli2 is required for the induction and migration of Xenopus laevis neural crest.
    Aybar MJ
    Mechanisms of development 154. (2018 Dec): 219-239.

    The sterol-sensing domain of Patched protein seems to control Smoothened activity through Patched vesicular trafficking.
    Guerrero I
    Current biology : CB 11.8 (2001 Apr 17): 601-7.

    Patched controls the Hedgehog gradient by endocytosis in a dynamin-dependent manner, but this internalization does not play a major role in signal transduction.
    Guerrero I
    Development (Cambridge, England) 131.10 (2004 May): 2395-408.

    The Drosophila ortholog of the human Wnt inhibitor factor Shifted controls the diffusion of lipid-modified Hedgehog.
    Guerrero I
    Developmental cell 8.2 (2005 Feb): 241-53.

    Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner.
    Jia J
    Developmental biology 366.2 (2012 Jun 15): 172-84.

    Hyperplastic discs differentially regulates the transcriptional outputs of hedgehog signaling.
    Lin X
    Mechanisms of development 133. (2014 Aug): 117-25.

    Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover.
    Chen D
    PLoS biology 11.11 (2013 Nov): e1001721.

    Detecting tagged Hedgehog with intracellular and extracellular immunocytochemistry for functional analysis.
    Guerrero I
    Methods in molecular biology (Clifton, N.J.) 397. (2007): 91-103.

    Non-cell autonomous control of apoptosis by ligand-independent Hedgehog signaling in Drosophila.
    Bergmann A
    Cell death and differentiation 20.2 (2013 Feb): 302-11.

    The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.
    Zhao Y
    The Journal of biological chemistry 289.32 (2014 Aug 8): 22333-41.

    Smoothened oligomerization/higher order clustering in lipid rafts is essential for high Hedgehog activity transduction.
    Zhao Y
    The Journal of biological chemistry 288.18 (2013 May 3): 12605-14.

    Transcription in the absence of histone H3.3.
    Basler K
    Current biology : CB 19.14 (2009 Jul 28): 1221-6.

    Dynamic phosphorylation of the kinesin Costal-2 in vivo reveals requirement of fused kinase activity for all levels of hedgehog signalling.
    Thérond PP
    Developmental biology 344.1 (2010 Aug 1): 119-28.

    Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation.
    O'Reilly AM
    The Journal of cell biology 191.5 (2010 Nov 29): 943-52.

    Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila.
    Jia J
    Methods in molecular biology (Clifton, N.J.) 1322. (2015): 45-60.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

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