Math1 (Atoh1)
Catalog Fields
Antigen: Math1 (Atoh1)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Johnson, J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UT Southwestern
Positive Tested Species Reactivity: Mouse
Depositors Notes: Doesn't work on Chicken. Expression in neuronal progenitor cells in dorsal neural tube, EGL of cerebellum, hair cells in inner ear, Merkel cells in skin. Doesn't appear to have a functional effect.
Antigen Molecular Weight: Predicted: 38 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Atoh1
Immunogen: Rcombinant Math1/GST fusion protein
Alternate Gene Names: Ath1
Alternate Antibody Name: Atoh1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P48985
Immunogen Sequence: Full length protein
Entrez Gene ID: 11921
Additional Characterization:
Antibody Registry ID: AB_10805299
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Math1 (Atoh1) was deposited to the DSHB by Johnson, J. (DSHB Hybridoma Product Math1 (Atoh1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
Kawakami T
The Angle orthodontist 75.6 (2005 Nov): 993-5.