Math1 (Atoh1)
Catalog Fields
Antigen: Math1 (Atoh1)
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Johnson, J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse
Depositors Institution: UT Southwestern Medical Center
Antigen Molecular Weight: Predicted: 38 kDa
Depositors Notes: Doesn't work on Chicken. Expression in neuronal progenitor cells in dorsal neural tube, EGL of cerebellum, hair cells in inner ear, Merkel cells in skin. Doesn't appear to have a functional effect.
Immunogen: Rcombinant Math1/GST fusion protein
Gene: Atoh1
Alternate Antibody Name: Atoh1
Alternate Gene Names: Ath1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: P48985
Epitope Location or Sequence:
Entrez Gene ID: 11921
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_10805299
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Math1 (Atoh1) was deposited to the DSHB by Johnson, J. (DSHB Hybridoma Product Math1 (Atoh1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
Kawakami T
The Angle orthodontist 75.6 (2005 Nov): 993-5.