Math1 (Atoh1)
Catalog Fields
Antigen: Math1 (Atoh1)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Johnson, J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UT Southwestern
Positive Tested Species Reactivity: Mouse
Depositors Notes: Doesn't work on Chicken. Expression in neuronal progenitor cells in dorsal neural tube, EGL of cerebellum, hair cells in inner ear, Merkel cells in skin. Doesn't appear to have a functional effect.
Predicted Species Reactivity:
Gene: Atoh1
Immunogen: Rcombinant Math1/GST fusion protein
Alternate Gene Names: Ath1
Alternate Antibody Name: Atoh1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P48985
Immunogen Sequence: Full length protein
Entrez Gene ID: 11921
Antibody Registry ID: AB_10805299
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Math1 (Atoh1) was deposited to the DSHB by Johnson, J. (DSHB Hybridoma Product Math1 (Atoh1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice.
Kawakami T
The Angle orthodontist 75.6 (2005 Nov): 993-5.