50.5A5

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$40.00
SKU: 50.5A5
View product citations for antibody 50.5A5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: LIM/homeobox protein LMX-1.2
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University, Howard Hughes Medical Institute
Positive Tested Species Reactivity: Mouse, Planaria
Depositors Notes: Stains the roof plate, floor plate, dorsal limb bud, and a subset of interneurons (nuclear)
Antigen Molecular Weight: Predicted : 45 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LMX1B
Immunogen: Recombinant 6× His-tagged LMX1 protein fragment (LIM domains and homeodomain)
Alternate Gene Names: LMX, LMX1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: LIM domains and homeodomain
Uniprot ID: P53413 
Immunogen Sequence: Partial protein
Entrez Gene ID: 396312 
Additional Characterization:
Antibody Registry ID: AB_2136850 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
50.5A5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 50.5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    Epitope Map References
    All References

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