50.5A5

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$40.00
SKU: 50.5A5
View product citations for antibody 50.5A5 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: LIM/homeobox protein LMX-1.2
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University, Howard Hughes Medical Institute
Positive Tested Species Reactivity: Mouse, Planaria
Depositors Notes: Stains the roof plate, floor plate, dorsal limb bud, and a subset of interneurons (nuclear)
Antigen Molecular Weight: Predicted : 45 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: LMX1B
Immunogen: Recombinant 6× His-tagged LMX1 protein fragment (LIM domains and homeodomain)
Alternate Gene Names: LMX, LMX1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: LIM domains and homeodomain
Uniprot ID: P53413 
Immunogen Sequence: Partial protein
Entrez Gene ID: 396312 
Additional Characterization:  
Antibody Registry ID: AB_2136850 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
50.5A5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 50.5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    Epitope Map References
    All References

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