Antigen: tubulin (alpha-)
Hybridoma Cells Available: Yes
Antigen Species: Naegleria gruberi
Depositor: Walsh, C.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Broad species, C. elegans, Drosophila melanogaster, Fish, Human, Mammal, Mouse, Plant, Protist, Rat, Worm
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: flagellar axoneme
Alternate Gene Names:
Alternate Antibody Name: AA-4.3
Alternate Antigen Name:
Myeloma Strain: P3-X63Ag8
Epitope Location or Sequence:
Uniprot ID: P11237
Immunogen Sequence: Total protein
Entrez Gene ID: 8851752
Antibody Registry ID: AB_579793
Additional Information: Initial characterization [PMID 6363422] performed on Naegleria gruberi tubulins suggested that this antibody target was beta-tubulin. Further characterization [PMID 3654753] demonstrated that the target was alpha-tubulin. This antibody may recognize more than one alpha-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AA4.3 was deposited to the DSHB by Walsh, C. (DSHB Hybridoma Product AA4.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).