6H4 anti-highwire

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$40.00
SKU: 6H4 anti-highwire
View product citations for antibody 6H4 anti-highwire on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: highwire
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2b
Depositors Institution: University of California, Berkeley
Host Species: mouse
Depositors Notes: Labels presynaptic structures. Specific to HIW, no staining was observed in hiw mutants.
Positive Tested Species Reactivity: Drosophila
Gene: hiw
Antigen Molecular Weight: Predicted: 566 kDa
Alternate Gene Names: CG32592
Immunogen: Recombinant 6× His-tagged highwire protein fragment (aa 2684–2818)
Clonality: Monoclonal
Alternate Antibody Name: 6H4
Epitope Mapped: Yes
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence: aa 2684–2818
Uniprot ID: Q9NB71 
Immunogen Sequence: Partial protein
Entrez Gene ID: 32429 
Additional Information: 6H4 was isolated during a screen for anti-highwire mAbs [PMID: 10839352] but has since been shown to cross-react with a second unidentified presynaptic epitope distinct from Hiw in hiw mutant [PMID: 16237161].
Antibody Registry ID: AB_528277 
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H4 anti-highwire was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 6H4 anti-highwire)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    IF References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.42 (2005 Oct 19): 9557-66.

    The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila.
    Rao Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.45 (2009 Nov 11): 14151-9.

    Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila.
    Salecker I
    Cell 128.5 (2007 Mar 9): 961-75.

    IHC References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Epitope Map References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    All References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.42 (2005 Oct 19): 9557-66.

    The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila.
    Rao Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.45 (2009 Nov 11): 14151-9.

    Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila.
    Salecker I
    Cell 128.5 (2007 Mar 9): 961-75.

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