6H4 anti-highwire
Catalog Fields
Product Name/ID: 6H4 anti-highwire
Available to For-Profits: Yes
Alternate Antibody Name: 6H4
Gene Name: hiw
Ab Isotype: MIgG2b
Gene Symbol:
Entrez Gene ID: 32429
Clonality: Monoclonal
Immunogen: Recombinant 6× His-tagged highwire protein fragment (aa 2684–2818)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3 x 63 Ag8.653
Epitope Mapped: Yes
Antigen Name: highwire
Epitope Location or Sequence: aa 2684–2818
Alternate Antigen Name:
Deposit Date: 3/14/2002
Antigen Molecular Weight: Predicted: 566 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Labels presynaptic structures. Specific to HIW, no staining was observed in hiw mutants.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: 6H4 was isolated during a screen for anti-highwire mAbs [PMID: 10839352] but has since been shown to cross-react with a second unidentified presynaptic epitope distinct from Hiw in hiw mutant [PMID: 16237161].
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H4 anti-highwire was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 6H4 anti-highwire)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
4 References
Initial Publication
Highwire regulates synaptic growth in Drosophila.
Goodman CS
Neuron 26.2 (2000 May): 313-29.