6H4 anti-highwire

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$40.00
SKU: 6H4 anti-highwire
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DSHB Data Sheet

Catalog Fields

Antigen: highwire
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Labels presynaptic structures. Specific to HIW, no staining was observed in hiw mutants.
Antigen Molecular Weight: Predicted: 566 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: hiw
Immunogen: Recombinant 6× His-tagged highwire protein fragment (aa 2684–2818)
Alternate Gene Names: CG32592
Alternate Antibody Name: 6H4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence: aa 2684–2818
Uniprot ID: Q9NB71 
Immunogen Sequence: Partial protein
Entrez Gene ID: 32429 
Additional Characterization:  
Antibody Registry ID: AB_528277 
Additional Information: 6H4 was isolated during a screen for anti-highwire mAbs [PMID: 10839352] but has since been shown to cross-react with a second unidentified presynaptic epitope distinct from Hiw in hiw mutant [PMID: 16237161].
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H4 anti-highwire was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 6H4 anti-highwire)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    IF References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.42 (2005 Oct 19): 9557-66.

    The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila.
    Rao Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.45 (2009 Nov 11): 14151-9.

    Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila.
    Salecker I
    Cell 128.5 (2007 Mar 9): 961-75.

    IHC References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Epitope Map References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    All References

    Highwire regulates synaptic growth in Drosophila.
    Goodman CS
    Neuron 26.2 (2000 May): 313-29.

    Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.42 (2005 Oct 19): 9557-66.

    The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila.
    Rao Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.45 (2009 Nov 11): 14151-9.

    Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila.
    Salecker I
    Cell 128.5 (2007 Mar 9): 961-75.

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