99.1-3A2

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$40.00
SKU: 99.1-3A2
View product citations for antibody 99.1-3A2 on CiteAb

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Available: 91

DSHB Data Sheet

Catalog Fields

Antigen: Homeobox even-skipped homolog protein 1/2
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse
Depositors Notes: This antibody recognizes Evx1 and Evx2
Antigen Molecular Weight: Predicted: 43 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Evx1
Immunogen: C-terminal peptide of Evx1 coupled to KLH
Alternate Gene Names:
Alternate Antibody Name: 3A2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminus
Uniprot ID: P23683 
Immunogen Sequence: peptide
Entrez Gene ID: 14028 
Additional Characterization:
Antibody Registry ID: AB_528231 
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
99.1-3A2 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 99.1-3A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord.
    Jessell TM
    Cell 97.7 (1999 Jun 25): 903-15.

    DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function.
    Kullander K
    Developmental biology 366.2 (2012 Jun 15): 279-89.

    Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Nedelec S
    Nature biotechnology 33.1 (2015 Jan): 89-96.

    Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development.
    Cai L
    Scientific reports 6. (2016 Dec 7): 38665.

    Generation of highly enriched V2a interneurons from mouse embryonic stem cells.
    Sakiyama-Elbert SE
    Experimental neurology 277. (2016 Mar): 305-316.

    Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Xiang M
    Developmental dynamics : an official publication of the American Association of Anatomists 244.1 (2015 Jan): 86-97.

    Characterization of Foxp2-expressing cells in the developing spinal cord.
    Senba E
    Neuroscience 162.4 (2009 Sep 15): 1150-62.

    Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
    Martin DM
    Molecular and cellular neurosciences 49.1 (2012 Jan): 32-43.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Suppression of interneuron programs and maintenance of selected spinal motor neuron fates by the transcription factor AML1/Runx1.
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

    IHC References
    Epitope Map References
    All References

    Self-organized formation of developing appendages from murine pluripotent stem cells.
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord.
    Jessell TM
    Cell 97.7 (1999 Jun 25): 903-15.

    DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function.
    Kullander K
    Developmental biology 366.2 (2012 Jun 15): 279-89.

    Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Nedelec S
    Nature biotechnology 33.1 (2015 Jan): 89-96.

    Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development.
    Cai L
    Scientific reports 6. (2016 Dec 7): 38665.

    Generation of highly enriched V2a interneurons from mouse embryonic stem cells.
    Sakiyama-Elbert SE
    Experimental neurology 277. (2016 Mar): 305-316.

    Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Xiang M
    Developmental dynamics : an official publication of the American Association of Anatomists 244.1 (2015 Jan): 86-97.

    Characterization of Foxp2-expressing cells in the developing spinal cord.
    Senba E
    Neuroscience 162.4 (2009 Sep 15): 1150-62.

    Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
    Martin DM
    Molecular and cellular neurosciences 49.1 (2012 Jan): 32-43.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Suppression of interneuron programs and maintenance of selected spinal motor neuron fates by the transcription factor AML1/Runx1.
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

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