99.1-3A2
Catalog Fields
Antigen: Homeobox even-skipped homolog protein 1/2
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse
Depositors Notes: This antibody recognizes Evx1 and Evx2
Antigen Molecular Weight: Predicted: 43 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Evx1
Immunogen: C-terminal peptide of Evx1 coupled to KLH
Alternate Gene Names:
Alternate Antibody Name: 3A2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminus
Uniprot ID: P23683
Immunogen Sequence: peptide
Entrez Gene ID: 14028
Additional Characterization:
Antibody Registry ID: AB_528231
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
99.1-3A2 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 99.1-3A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord.
Jessell TM
Cell 97.7 (1999 Jun 25): 903-15.