99.1-3A2
Catalog Fields
Antigen: Homeobox even-skipped homolog protein 1/2
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse
Depositors Notes: This antibody recognizes Evx1 and Evx2
Predicted Species Reactivity:
Gene: Evx1
Immunogen: C-terminal peptide of Evx1 coupled to KLH
Alternate Gene Names:
Alternate Antibody Name: 3A2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminus
Uniprot ID: P23683
Immunogen Sequence: peptide
Entrez Gene ID: 14028
Antibody Registry ID: AB_528231
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
99.1-3A2 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 99.1-3A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord.
Jessell TM
Cell 97.7 (1999 Jun 25): 903-15.