C566.9

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$40.00
SKU: C566.9
View product citations for antibody C566.9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Coracle (common)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fehon, R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Chicago
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: multiple isoforms from 78 to 184 kDa. Apparent: 200 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cora
Immunogen: Recombinant Coracle FERM domain (aa 72-397)/GST fusion protein
Alternate Gene Names: CG11949, Cor, Coracle, D4.1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 72-397 of isoform 1 (Uniprot ID: Q9V8R9-1).
Uniprot ID: Q9V8R9 
Immunogen Sequence: Partial protein
Entrez Gene ID: 37205 
Additional Characterization:  
Antibody Registry ID: AB_1161642 
Additional Information: This antibody will recognize all coracle isoforms except maybe isoform 5 (Uniprot ID: Q9V8R9-5). This antigen is also known as protein 4.1 homolog and is a component of septate junctions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C566.9 was deposited to the DSHB by Fehon, R. (DSHB Hybridoma Product C566.9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 545-57.

    Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis.
    Riechmann V
    The Journal of cell biology 199.7 (2012 Dec 24): 1131-43.

    The Drosophila shark tyrosine kinase is required for embryonic dorsal closure.
    Stanley ER
    Genes & development 14.5 (2000 Mar 1): 604-14.

    Epitope Map References
    All References

    A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 545-57.

    Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis.
    Riechmann V
    The Journal of cell biology 199.7 (2012 Dec 24): 1131-43.

    The Drosophila shark tyrosine kinase is required for embryonic dorsal closure.
    Stanley ER
    Genes & development 14.5 (2000 Mar 1): 604-14.

    Ribbon regulates morphogenesis of the Drosophila embryonic salivary gland through transcriptional activation and repression.
    Andrew DJ
    Developmental biology 409.1 (2016 Jan 1): 234-250.

    Neuroendocrine regulation of Drosophila metamorphosis requires TGFbeta/Activin signaling.
    O'Connor MB
    Development (Cambridge, England) 138.13 (2011 Jul): 2693-703.

    Rac1 controls epithelial tube length through the apical secretion and polarity pathways.
    Laprise P
    Biology open 5.1 (2015 Dec 23): 49-54.

    A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
    Tanentzapf G
    Development (Cambridge, England) 142.2 (2015 Jan 15): 268-81.

    Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function.
    Renault AD
    Development (Cambridge, England) 139.14 (2012 Jul): 2535-46.

    Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.
    Ghabrial AS
    Developmental cell 25.5 (2013 Jun 10): 507-19.

    Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization.
    Tepass U
    Nature cell biology 5.1 (2003 Jan): 46-52.

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