C615.16

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$40.00
SKU: C615.16
View product citations for antibody C615.16 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Coracle (heavy isoform)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fehon, R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Chicago
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 184 kDa; Apparent: 200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cora
Immunogen: Recombinant GST fusion protein
Alternate Gene Names: CG11949, Cor, Coracle, D4.1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 608-1285 of isoform 1 (Uniprot ID: Q9V8R9-1).
Uniprot ID: Q9V8R9 
Immunogen Sequence: Coralcle C-terminal region a.a. 608-1285
Entrez Gene ID: 37205 
Additional Characterization:  
Antibody Registry ID: AB_1161644 
Additional Information: This antibody only recognizes coracle isoform 1 (Uniprot ID: Q9V8R9-1). This antigen is also known as protein 4.1 homolog and is a component of septate junctions. FBgn0010434 / RRID:AB_1161644
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C615.16 was deposited to the DSHB by Fehon, R. (DSHB Hybridoma Product C615.16)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
    Choe KM
    PLoS genetics 10.10 (2014 Oct): e1004683.

    Without children is required for Stat-mediated zfh1 transcription and for germline stem cell differentiation.
    Gilboa L
    Development (Cambridge, England) 141.13 (2014 Jul): 2602-10.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster.
    Merritt DJ
    Arthropod structure & development 42.5 (2013 Sep): 385-93.

    A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut.
    Furuse M
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4923-33.

    Btk-dependent epithelial cell rearrangements contribute to the invagination of nearby tubular structures in the posterior spiracles of Drosophila.
    Strigini M
    Developmental biology 396.1 (2014 Dec 1): 42-56.

    A tetraspanin regulates septate junction formation in Drosophila midgut.
    Furuse M
    Journal of cell science 129.6 (2016 Mar 15): 1155-64.

    Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
    Choe KM
    PLoS genetics 10.10 (2014 Oct): e1004683.

    Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila.
    Furuse M
    Journal of cell science 125.Pt 8 (2012 Apr 15): 1980-90.

    Rac1 controls epithelial tube length through the apical secretion and polarity pathways.
    Laprise P
    Biology open 5.1 (2015 Dec 23): 49-54.

    A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
    Tanentzapf G
    Development (Cambridge, England) 142.2 (2015 Jan 15): 268-81.

    Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function.
    Renault AD
    Development (Cambridge, England) 139.14 (2012 Jul): 2535-46.

    Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.
    Ghabrial AS
    Developmental cell 25.5 (2013 Jun 10): 507-19.

    Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization.
    Tepass U
    Nature cell biology 5.1 (2003 Jan): 46-52.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Coordination of Septate Junctions Assembly and Completion of Cytokinesis in Proliferative Epithelial Tissues.
    Le Borgne R
    Current biology : CB 28.9 (2018 May 7): 1380-1391.e4.

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling.
    Chien CT
    Current biology : CB 29.9 (2019 May 6): 1445-1459.e3.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    WB References
    IHC References

    The Drosophila shark tyrosine kinase is required for embryonic dorsal closure.
    Stanley ER
    Genes & development 14.5 (2000 Mar 1): 604-14.

    Epitope Map References
    All References

    The Drosophila shark tyrosine kinase is required for embryonic dorsal closure.
    Stanley ER
    Genes & development 14.5 (2000 Mar 1): 604-14.

    Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
    Choe KM
    PLoS genetics 10.10 (2014 Oct): e1004683.

    Without children is required for Stat-mediated zfh1 transcription and for germline stem cell differentiation.
    Gilboa L
    Development (Cambridge, England) 141.13 (2014 Jul): 2602-10.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster.
    Merritt DJ
    Arthropod structure & development 42.5 (2013 Sep): 385-93.

    A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut.
    Furuse M
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4923-33.

    Btk-dependent epithelial cell rearrangements contribute to the invagination of nearby tubular structures in the posterior spiracles of Drosophila.
    Strigini M
    Developmental biology 396.1 (2014 Dec 1): 42-56.

    A tetraspanin regulates septate junction formation in Drosophila midgut.
    Furuse M
    Journal of cell science 129.6 (2016 Mar 15): 1155-64.

    Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila.
    Furuse M
    Journal of cell science 125.Pt 8 (2012 Apr 15): 1980-90.

    Rac1 controls epithelial tube length through the apical secretion and polarity pathways.
    Laprise P
    Biology open 5.1 (2015 Dec 23): 49-54.

    A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
    Tanentzapf G
    Development (Cambridge, England) 142.2 (2015 Jan 15): 268-81.

    Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function.
    Renault AD
    Development (Cambridge, England) 139.14 (2012 Jul): 2535-46.

    Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.
    Ghabrial AS
    Developmental cell 25.5 (2013 Jun 10): 507-19.

    Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization.
    Tepass U
    Nature cell biology 5.1 (2003 Jan): 46-52.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Coordination of Septate Junctions Assembly and Completion of Cytokinesis in Proliferative Epithelial Tissues.
    Le Borgne R
    Current biology : CB 28.9 (2018 May 7): 1380-1391.e4.

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling.
    Chien CT
    Current biology : CB 29.9 (2019 May 6): 1445-1459.e3.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 545-57.

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