mMaC myomesin B4

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$40.00
SKU: mMaC myomesin B4
View product citations for antibody mMaC myomesin B4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: myomesin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Perriard, J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: ETH-Honggerberg / Institute of Cell Biology
Positive Tested Species Reactivity: Chicken, Fish, Mouse, Rabbit, Vertebrates, Xenopus, Zebrafish
Depositors Notes:
Antigen Molecular Weight: 182.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYOM1
Immunogen: M-band protein fraction from pectoralis
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-NS1/lAg4-1
Epitope Location or Sequence:
Uniprot ID: Q90724 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 395805 
Additional Characterization:  
Antibody Registry ID: AB_760349 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mMaC myomesin B4 was deposited to the DSHB by Perriard, J.-C. (DSHB Hybridoma Product mMaC myomesin B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication

    A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies.
    Eppenberger HM
    The Journal of cell biology 98.2 (1984 Feb): 518-24.

    IF References

    Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos.
    Zhong Y
    Proceedings of the National Academy of Sciences of the United States of America 105.2 (2008 Jan 15): 554-9.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    RYBP is important for cardiac progenitor cell development and sarcomere formation.
    Pirity MK
    PloS one 15.7 (2020): e0235922.

    A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies.
    Eppenberger HM
    The Journal of cell biology 98.2 (1984 Feb): 518-24.

    Structure before function: myosin binding protein-C slow is a structural protein with regulatory properties.
    Kontrogianni-Konstantopoulos A
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology . (2018 Jun 6): fj201800624R.

    Cardiac MLC2 kinase is localized to the Z-disc and interacts with α-actinin2.
    Kasahara H
    Scientific reports 9.1 (2019 Aug 29): 12580.

    Differences in titin segmental elongation between passive and active stretch in skeletal muscle.
    Herzog W
    The Journal of experimental biology 220.Pt 23 (2017 Dec 1): 4418-4425.

    Determination of the source of SHG verniers in zebrafish skeletal muscle.
    Pantazis P
    Scientific reports 5. (2015 Dec 11): 18119.

    Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2.
    Manganiello V
    The Journal of biological chemistry 290.11 (2015 Mar 13): 6763-76.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    WB References

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Structure before function: myosin binding protein-C slow is a structural protein with regulatory properties.
    Kontrogianni-Konstantopoulos A
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology . (2018 Jun 6): fj201800624R.

    Cardiac MLC2 kinase is localized to the Z-disc and interacts with α-actinin2.
    Kasahara H
    Scientific reports 9.1 (2019 Aug 29): 12580.

    Heterozygous <i>Mylk3</i> Knockout Mice Partially Recapitulate Human DCM With Heterozygous <i>MYLK3</i> Mutations.
    Kasahara H
    Frontiers in physiology 10. (2019): 696.

    Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers.
    Bang ML
    Cardiovascular research 107.2 (2015 Jul 15): 216-25.

    IHC References

    Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles.
    Du SJ
    Journal of genetics and genomics = Yi chuan xue bao 39.2 (2012 Feb): 69-80.

    Smyd1b_tv1, a key regulator of sarcomere assembly, is localized on the M-line of skeletal muscle fibers.
    Du SJ
    PloS one 6.12 (2011): e28524.

    Loss of Smyhc1 or Hsp90alpha1 function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos.
    Du SJ
    PloS one 5.1 (2010 Jan 1): e8416.

    Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos.
    Zhong Y
    Proceedings of the National Academy of Sciences of the United States of America 105.2 (2008 Jan 15): 554-9.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Structure before function: myosin binding protein-C slow is a structural protein with regulatory properties.
    Kontrogianni-Konstantopoulos A
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology . (2018 Jun 6): fj201800624R.

    Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death.
    Cooper KL
    eLife 8. (2019 Oct 15): .

    Cardiac MLC2 kinase is localized to the Z-disc and interacts with α-actinin2.
    Kasahara H
    Scientific reports 9.1 (2019 Aug 29): 12580.

    Defective sarcomere assembly in <i>smyd1a</i> and <i>smyd1b</i> zebrafish mutants.
    Du S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33.5 (2019 May): 6209-6225.

    Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease.
    Red-Horse K
    Nature communications 9.1 (2018 Jan 25): 368.

    Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2.
    Manganiello V
    The Journal of biological chemistry 290.11 (2015 Mar 13): 6763-76.

    ELISA References

    A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies.
    Eppenberger HM
    The Journal of cell biology 98.2 (1984 Feb): 518-24.

    FFPE References
    All References

    Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles.
    Du SJ
    Journal of genetics and genomics = Yi chuan xue bao 39.2 (2012 Feb): 69-80.

    Smyd1b_tv1, a key regulator of sarcomere assembly, is localized on the M-line of skeletal muscle fibers.
    Du SJ
    PloS one 6.12 (2011): e28524.

    Loss of Smyhc1 or Hsp90alpha1 function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos.
    Du SJ
    PloS one 5.1 (2010 Jan 1): e8416.

    Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos.
    Zhong Y
    Proceedings of the National Academy of Sciences of the United States of America 105.2 (2008 Jan 15): 554-9.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Structure before function: myosin binding protein-C slow is a structural protein with regulatory properties.
    Kontrogianni-Konstantopoulos A
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology . (2018 Jun 6): fj201800624R.

    Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death.
    Cooper KL
    eLife 8. (2019 Oct 15): .

    Cardiac MLC2 kinase is localized to the Z-disc and interacts with α-actinin2.
    Kasahara H
    Scientific reports 9.1 (2019 Aug 29): 12580.

    Defective sarcomere assembly in <i>smyd1a</i> and <i>smyd1b</i> zebrafish mutants.
    Du S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33.5 (2019 May): 6209-6225.

    Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease.
    Red-Horse K
    Nature communications 9.1 (2018 Jan 25): 368.

    Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2.
    Manganiello V
    The Journal of biological chemistry 290.11 (2015 Mar 13): 6763-76.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    RYBP is important for cardiac progenitor cell development and sarcomere formation.
    Pirity MK
    PloS one 15.7 (2020): e0235922.

    A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies.
    Eppenberger HM
    The Journal of cell biology 98.2 (1984 Feb): 518-24.

    Differences in titin segmental elongation between passive and active stretch in skeletal muscle.
    Herzog W
    The Journal of experimental biology 220.Pt 23 (2017 Dec 1): 4418-4425.

    Determination of the source of SHG verniers in zebrafish skeletal muscle.
    Pantazis P
    Scientific reports 5. (2015 Dec 11): 18119.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Heterozygous <i>Mylk3</i> Knockout Mice Partially Recapitulate Human DCM With Heterozygous <i>MYLK3</i> Mutations.
    Kasahara H
    Frontiers in physiology 10. (2019): 696.

    Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers.
    Bang ML
    Cardiovascular research 107.2 (2015 Jul 15): 216-25.

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