a6F
Catalog Fields
Clone ID/Product Name: a6F
Available to For-Profits: Yes
Alternate Antibody Name: 6F; alpha6F; Atp1a1
Gene Symbol: ATP1A1
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Entrez Gene ID: 396530
Clonality: Monoclonal
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: SP 2/0 Ag8
Epitope Mapped: Yes
Antigen Name: ATPase, (Na(+) K(+)) alpha-1 subunit
Epitope Location or Sequence: N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].
Alternate Antigen Name:
Deposit Date: 2/2/1996
Antigen Molecular Weight: 112 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine
Antigen Species: Chicken
Depositor Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Axolotl, Canine, Fish, Human, Mouse, Porcine, Rat, Torpedo, Zebrafish
Additional Information: Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot