a6F

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SKU: a6F
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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian, Canine, Fish, Human, Mouse, Porcine, Rat, Torpedo, Zebrafish
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].
Antigen Molecular Weight: 112 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ATP1A1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Alternate Gene Names:
Alternate Antibody Name: 6F; alpha6F; Atp1a1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0 Ag8
Epitope Location or Sequence: N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].
Uniprot ID: P09572 
Immunogen Sequence: Total protein
Entrez Gene ID: 396530 
Additional Characterization:
Antibody Registry ID: AB_528092 
Additional Information: Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a6F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a6F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

30 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of biological chemistry
    Fambrough DM
    The Journal of biological chemistry 263.9 (1988 Mar 25): 4347-54.

    IF References

    Development (Cambridge, England)
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Histology and histopathology
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    Neuroscience
    Martín-Vasallo P
    Neuroscience 129.3 (2004): 691-702.

    The Journal of biological chemistry
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    The Journal of biological chemistry
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    The Journal of biological chemistry
    Xie Z
    The Journal of biological chemistry 288.8 (2013 Feb 22): 5803-14.

    The Journal of biological chemistry
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Development (Cambridge, England)
    Sive H
    Development (Cambridge, England) 132.9 (2005 May): 2057-67.

    PloS one
    Apodaca G
    PloS one 7.7 (2012): e41816.

    Journal of the American Society of Nephrology : JASN
    Drummond IA
    Journal of the American Society of Nephrology : JASN 17.10 (2006 Oct): 2706-18.

    PloS one
    Brismar H
    PloS one 13.4 (2018): e0195825.

    Neurobiology of disease
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    WB References

    Physiological reports
    Pierre SV
    Physiological reports 4.19 (2016 Oct): .

    The Journal of biological chemistry
    Brandes RP
    The Journal of biological chemistry 291.13 (2016 Mar 25): 7045-59.

    The Journal of biological chemistry
    Gumá A
    The Journal of biological chemistry 276.21 (2001 May 25): 18257-64.

    Histology and histopathology
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    The Journal of biological chemistry
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    American journal of physiology. Renal physiology
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    The Journal of biological chemistry
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    Molecular reproduction and development
    James PF
    Molecular reproduction and development 73.1 (2006 Jan): 101-15.

    The Journal of biological chemistry
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Journal of experimental zoology. Part A, Ecological genetics and physiology
    Lee TH
    Journal of experimental zoology. Part A, Ecological genetics and physiology 311.7 (2009 Aug 1): 521-9.

    PloS one
    Michalakis S
    PloS one 5.1 (2010 Jan 29): e8969.

    Molecular brain
    Waxman SG
    Molecular brain 11.1 (2018 Aug 7): 45.

    Glia
    Roussa E
    Glia 67.12 (2019 Dec): 2264-2278.

    Neurobiology of disease
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    IHC References

    Development (Cambridge, England)
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Histology and histopathology
    Mobasheri R
    Histology and histopathology 16.1 (2001 Jan): 141-54.

    American journal of physiology. Renal physiology
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    Developmental biology
    Kispert A
    Developmental biology 359.1 (2011 Nov 1): 95-107.

    Reproductive sciences (Thousand Oaks, Calif.)
    Mobasheri A
    Reproductive sciences (Thousand Oaks, Calif.) 17.4 (2010 Apr): 366-76.

    Frontiers in physiology
    Hidalgo C
    Frontiers in physiology 6. (2015): 105.

    IP References

    The Journal of biological chemistry
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    The Journal of biological chemistry
    Hedden L
    The Journal of biological chemistry 285.47 (2010 Nov 19): 36330-8.

    ELISA References

    The Journal of biological chemistry
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    Epitope Map References

    The Journal of biological chemistry
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    All References

    Development (Cambridge, England)
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Histology and histopathology
    Mobasheri R
    Histology and histopathology 16.1 (2001 Jan): 141-54.

    American journal of physiology. Renal physiology
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    Developmental biology
    Kispert A
    Developmental biology 359.1 (2011 Nov 1): 95-107.

    Reproductive sciences (Thousand Oaks, Calif.)
    Mobasheri A
    Reproductive sciences (Thousand Oaks, Calif.) 17.4 (2010 Apr): 366-76.

    Frontiers in physiology
    Hidalgo C
    Frontiers in physiology 6. (2015): 105.

    Histology and histopathology
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    Neuroscience
    Martín-Vasallo P
    Neuroscience 129.3 (2004): 691-702.

    The Journal of biological chemistry
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    The Journal of biological chemistry
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    The Journal of biological chemistry
    Xie Z
    The Journal of biological chemistry 288.8 (2013 Feb 22): 5803-14.

    The Journal of biological chemistry
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Development (Cambridge, England)
    Sive H
    Development (Cambridge, England) 132.9 (2005 May): 2057-67.

    PloS one
    Apodaca G
    PloS one 7.7 (2012): e41816.

    Journal of the American Society of Nephrology : JASN
    Drummond IA
    Journal of the American Society of Nephrology : JASN 17.10 (2006 Oct): 2706-18.

    PloS one
    Brismar H
    PloS one 13.4 (2018): e0195825.

    Neurobiology of disease
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    Physiological reports
    Pierre SV
    Physiological reports 4.19 (2016 Oct): .

    The Journal of biological chemistry
    Brandes RP
    The Journal of biological chemistry 291.13 (2016 Mar 25): 7045-59.

    The Journal of biological chemistry
    Gumá A
    The Journal of biological chemistry 276.21 (2001 May 25): 18257-64.

    The Journal of biological chemistry
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    Molecular reproduction and development
    James PF
    Molecular reproduction and development 73.1 (2006 Jan): 101-15.

    Journal of experimental zoology. Part A, Ecological genetics and physiology
    Lee TH
    Journal of experimental zoology. Part A, Ecological genetics and physiology 311.7 (2009 Aug 1): 521-9.

    PloS one
    Michalakis S
    PloS one 5.1 (2010 Jan 29): e8969.

    Molecular brain
    Waxman SG
    Molecular brain 11.1 (2018 Aug 7): 45.

    Glia
    Roussa E
    Glia 67.12 (2019 Dec): 2264-2278.

    The Journal of biological chemistry
    Hedden L
    The Journal of biological chemistry 285.47 (2010 Nov 19): 36330-8.

    The Journal of biological chemistry
    Fambrough DM
    The Journal of biological chemistry 263.9 (1988 Mar 25): 4347-54.

    BJU international
    Djamgoz MB
    BJU international 92.7 (2003 Nov): 793-802.

    The European journal of neuroscience
    Catsicas S
    The European journal of neuroscience 12.6 (2000 Jun): 1913-23.

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