a6F

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SKU: a6F
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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) alpha-1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian, Canine, Fish, Human, Mouse, Porcine, Rat, Torpedo, Zebrafish
Depositors Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].
Antigen Molecular Weight: 112 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP1A1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Alternate Gene Names:
Alternate Antibody Name: 6F; alpha6F; Atp1a1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0 Ag8
Epitope Location or Sequence: N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].
Uniprot ID: P09572 
Immunogen Sequence: Total protein
Entrez Gene ID: 396530 
Additional Characterization:  
Antibody Registry ID: AB_528092 
Additional Information: Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a6F was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a6F)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

30 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function.
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Beta1-integrins co-localize with Na, K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in mechanoreceptor complexes of mouse limb-bud chondrocytes.
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system.
    Martín-Vasallo P
    Neuroscience 129.3 (2004): 691-702.

    Loss of caveolin-1 impairs retinal function due to disturbance of subretinal microenvironment.
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3.
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    Expression of mutant α1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction.
    Xie Z
    The Journal of biological chemistry 288.8 (2013 Feb 22): 5803-14.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products.
    Sive H
    Development (Cambridge, England) 132.9 (2005 May): 2057-67.

    Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity.
    Apodaca G
    PloS one 7.7 (2012): e41816.

    Polycystin-2 immunolocalization and function in zebrafish.
    Drummond IA
    Journal of the American Society of Nephrology : JASN 17.10 (2006 Oct): 2706-18.

    Quantification of endogenous and exogenous protein expressions of Na,K-ATPase with super-resolution PALM/STORM imaging.
    Brismar H
    PloS one 13.4 (2018): e0195825.

    Factors in the disease severity of ATP1A3 mutations: Impairment, misfolding, and allele competition.
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    WB References

    Ischemia/reperfusion-induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+-ATPase: protection by ouabain preconditioning.
    Pierre SV
    Physiological reports 4.19 (2016 Oct): .

    The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein.
    Brandes RP
    The Journal of biological chemistry 291.13 (2016 Mar 25): 7045-59.

    A novel role of neuregulin in skeletal muscle. Neuregulin stimulates glucose uptake, glucose transporter translocation, and transporter expression in muscle cells.
    Gumá A
    The Journal of biological chemistry 276.21 (2001 May 25): 18257-64.

    Beta1-integrins co-localize with Na, K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in mechanoreceptor complexes of mouse limb-bud chondrocytes.
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit.
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition.
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    Loss of caveolin-1 impairs retinal function due to disturbance of subretinal microenvironment.
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    The human Na,K-ATPase alpha 4 isoform is a ouabain-sensitive alpha isoform that is expressed in sperm.
    James PF
    Molecular reproduction and development 73.1 (2006 Jan): 101-15.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities.
    Lee TH
    Journal of experimental zoology. Part A, Ecological genetics and physiology 311.7 (2009 Aug 1): 521-9.

    The retinitis pigmentosa mutation c.3444+1G>A in CNGB1 results in skipping of exon 32.
    Michalakis S
    PloS one 5.1 (2010 Jan 29): e8969.

    Multiple myosin motors interact with sodium/potassium-ATPase alpha 1 subunits.
    Waxman SG
    Molecular brain 11.1 (2018 Aug 7): 45.

    Functional expression of electrogenic sodium bicarbonate cotransporter 1 (NBCe1) in mouse cortical astrocytes is dependent on S255-257 and regulated by mTOR.
    Roussa E
    Glia 67.12 (2019 Dec): 2264-2278.

    Factors in the disease severity of ATP1A3 mutations: Impairment, misfolding, and allele competition.
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    IHC References

    Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function.
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Isoforms of Na+, K+-ATPase in human prostate; specificity of expression and apical membrane polarization.
    Mobasheri R
    Histology and histopathology 16.1 (2001 Jan): 141-54.

    Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition.
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    Impaired stria vascularis integrity upon loss of E-cadherin in basal cells.
    Kispert A
    Developmental biology 359.1 (2011 Nov 1): 95-107.

    Expression and distribution of Na, K-ATPase isoforms in the human uterus.
    Mobasheri A
    Reproductive sciences (Thousand Oaks, Calif.) 17.4 (2010 Apr): 366-76.

    Cholesterol removal from adult skeletal muscle impairs excitation-contraction coupling and aging reduces caveolin-3 and alters the expression of other triadic proteins.
    Hidalgo C
    Frontiers in physiology 6. (2015): 105.

    IP References

    A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3.
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells.
    Hedden L
    The Journal of biological chemistry 285.47 (2010 Nov 19): 36330-8.

    ELISA References
    Epitope Map References
    All References

    Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function.
    Fishman MC
    Development (Cambridge, England) 125.23 (1998 Dec): 4655-67.

    Isoforms of Na+, K+-ATPase in human prostate; specificity of expression and apical membrane polarization.
    Mobasheri R
    Histology and histopathology 16.1 (2001 Jan): 141-54.

    Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition.
    Friberg P
    American journal of physiology. Renal physiology 290.5 (2006 May): F1260-9.

    Impaired stria vascularis integrity upon loss of E-cadherin in basal cells.
    Kispert A
    Developmental biology 359.1 (2011 Nov 1): 95-107.

    Expression and distribution of Na, K-ATPase isoforms in the human uterus.
    Mobasheri A
    Reproductive sciences (Thousand Oaks, Calif.) 17.4 (2010 Apr): 366-76.

    Cholesterol removal from adult skeletal muscle impairs excitation-contraction coupling and aging reduces caveolin-3 and alters the expression of other triadic proteins.
    Hidalgo C
    Frontiers in physiology 6. (2015): 105.

    Beta1-integrins co-localize with Na, K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in mechanoreceptor complexes of mouse limb-bud chondrocytes.
    Mobasheri A
    Histology and histopathology 18.2 (2003 Apr): 343-51.

    Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system.
    Martín-Vasallo P
    Neuroscience 129.3 (2004): 691-702.

    Loss of caveolin-1 impairs retinal function due to disturbance of subretinal microenvironment.
    Elliott MH
    The Journal of biological chemistry 287.20 (2012 May 11): 16424-34.

    A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3.
    Aperia A
    The Journal of biological chemistry 288.4 (2013 Jan 25): 2734-43.

    Expression of mutant α1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction.
    Xie Z
    The Journal of biological chemistry 288.8 (2013 Feb 22): 5803-14.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products.
    Sive H
    Development (Cambridge, England) 132.9 (2005 May): 2057-67.

    Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity.
    Apodaca G
    PloS one 7.7 (2012): e41816.

    Polycystin-2 immunolocalization and function in zebrafish.
    Drummond IA
    Journal of the American Society of Nephrology : JASN 17.10 (2006 Oct): 2706-18.

    Quantification of endogenous and exogenous protein expressions of Na,K-ATPase with super-resolution PALM/STORM imaging.
    Brismar H
    PloS one 13.4 (2018): e0195825.

    Factors in the disease severity of ATP1A3 mutations: Impairment, misfolding, and allele competition.
    Sweadner KJ
    Neurobiology of disease 132. (2019 Dec): 104577.

    Ischemia/reperfusion-induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+-ATPase: protection by ouabain preconditioning.
    Pierre SV
    Physiological reports 4.19 (2016 Oct): .

    The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein.
    Brandes RP
    The Journal of biological chemistry 291.13 (2016 Mar 25): 7045-59.

    A novel role of neuregulin in skeletal muscle. Neuregulin stimulates glucose uptake, glucose transporter translocation, and transporter expression in muscle cells.
    Gumá A
    The Journal of biological chemistry 276.21 (2001 May 25): 18257-64.

    Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit.
    Sweadner KJ
    The Journal of biological chemistry 271.38 (1996 Sep 20): 23407-17.

    The human Na,K-ATPase alpha 4 isoform is a ouabain-sensitive alpha isoform that is expressed in sperm.
    James PF
    Molecular reproduction and development 73.1 (2006 Jan): 101-15.

    Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities.
    Lee TH
    Journal of experimental zoology. Part A, Ecological genetics and physiology 311.7 (2009 Aug 1): 521-9.

    The retinitis pigmentosa mutation c.3444+1G>A in CNGB1 results in skipping of exon 32.
    Michalakis S
    PloS one 5.1 (2010 Jan 29): e8969.

    Multiple myosin motors interact with sodium/potassium-ATPase alpha 1 subunits.
    Waxman SG
    Molecular brain 11.1 (2018 Aug 7): 45.

    Functional expression of electrogenic sodium bicarbonate cotransporter 1 (NBCe1) in mouse cortical astrocytes is dependent on S255-257 and regulated by mTOR.
    Roussa E
    Glia 67.12 (2019 Dec): 2264-2278.

    Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells.
    Hedden L
    The Journal of biological chemistry 285.47 (2010 Nov 19): 36330-8.

    Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.
    Fambrough DM
    The Journal of biological chemistry 263.9 (1988 Mar 25): 4347-54.

    Expression and cellular localization of Na,K-ATPase isoforms in the rat ventral prostate.
    Djamgoz MB
    BJU international 92.7 (2003 Nov): 793-802.

    Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking.
    Catsicas S
    The European journal of neuroscience 12.6 (2000 Jun): 1913-23.

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