Antibody Name: a6F
Antigen:ATPase, (Na(+) K(+)) alpha-1 subunit
Confocal microscope image of a temporal bone cross section containing the cochlea from a common marmoset fetus labeled with DSHB antibody a6F (white). PMID: 37675123, Fig. 5C.
Catalog Fields
Identity
Clone ID / Antibody Name: a6F
Alternate Antibody Name: 6F; alpha6F; Atp1a1
Antigen Name: ATPase, (Na(+) K(+)) alpha-1 subunit
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: ATP1A1
Entrez Gene ID: 396530
Antigen Sequence: —
Antigen Species: Chicken
Antigen Mol. Weight: 112 kDa
Uniprot ID: P09572
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG2a, kappa light chain
Myeloma Strain: SP 2/0 Ag8
Antibody Registry / RRID: AB_528092
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Immunogen Sequence: Total protein
Epitope Mapped: Yes
Epitope Location / Seq: N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].
Clone: —
Additional Info: Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Avian, Axolotl, Canine, Fish, Human, Mouse, Porcine, Rat, Torpedo, Zebrafish
Predicted Reactivity: —
Depositor
Depositor Name: D.M. Fambrough
Institution: Johns Hopkins School of Medicine
Deposit Date: February 2, 1996
Depositor Notes: Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].
Recommended Applications
ELISA
FFPE
IF
IHC
IP
WB
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