Antigen: Green fluorescent protein (GFP)
Available to For-Profits: Yes
Antigen Species: Aequorea victoria
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1, kappa light chain
Host Species: mouse
Positive Tested Species Reactivity: Aequorea victoria
Depositors Institution: University of Iowa
Antigen Molecular Weight: 26.8 Kda
Depositors Notes: DSHB-GFP-1D2 recognizes both denatured and native GFP protein.
Immunogen: Recombinant GFP expressed in E. coli
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Uniprot ID: P42212
Epitope Location or Sequence:
Entrez Gene ID: 7011691
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2617419
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: This antibody recognizes both denatured and native GFP. RRID:AB_2617419
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-1D2 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-1D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).