Antigen: Green fluorescent protein (GFP)
Hybridoma Cells Available: No
Antigen Species: Aequorea victoria
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: DSHB-GFP-1D2 recognizes both denatured and native GFP protein.
Positive Tested Species Reactivity: Aequorea victoria
Antigen Molecular Weight: 26.8 Kda
Immunogen: Recombinant GFP expressed in E. coli
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Immunogen Sequence: Full length protein
Uniprot ID: P42212
Additional Information: This antibody recognizes both denatured and native GFP. RRID:AB_2617419
Entrez Gene ID: 7011691
Antibody Registry ID: AB_2617419
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-1D2 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-1D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).