P5A11

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$40.00
SKU: P5A11
View product citations for antibody P5A11 on CiteAb

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Available: 142

DSHB Data Sheet

Catalog Fields

Antigen: 6XHis peptide, linear
Hybridoma Cells Available: Yes
Antigen Species: Protein Tag
Depositor: Wayner, E.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Protein tag
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: linear 6XHis peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: his-his-his-his-his-his peptide
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619591 
Additional Information:
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5A11 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References
    All References

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