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SKU: P5A11
View product citations for antibody P5A11 on CiteAb

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Available: 95

DSHB Data Sheet

Catalog Fields

Antigen: 6XHis peptide, linear
Hybridoma Cells Available: Yes
Antigen Species: Protein Tag
Depositor: Wayner, E.A.
Isotype: MIgG1
Depositors Institution: Fred Hutchison Cancer Research Center,
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Protein tag
Epitope Mapped: No
Immunogen: linear 6XHis peptide
Immunogen Sequence: his-his-his-his-his-his peptide
Myeloma Strain: NS-1/FOX-NY
Antibody Registry ID: AB_2619591 
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5A11 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References
    All References

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