H5

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$40.00
SKU: H5
View product citations for antibody H5 on CiteAb

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Available: 106

DSHB Data Sheet

Catalog Fields

Antigen: Vimentin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Sanes, J.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: Avian, Chicken, Lizard, Mouse, Planaria, Turtle, Zebrafish
Depositors Notes:
Antigen Molecular Weight: Predicted: 53 kDa; Apparent: 53 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: VIM
Immunogen: Chick optic tectum homogenate (E10)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Fox NY
Epitope Location or Sequence:
Uniprot ID: P09654 
Immunogen Sequence: Full length protein
Entrez Gene ID: 420519 
Additional Characterization:  
Antibody Registry ID: AB_528506 
Additional Information: Vimentin is a radial glial cell marker.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    IF References

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3.
    Bogerd J
    Endocrinology 156.10 (2015 Oct): 3804-17.

    Interaction between SCO-spondin and low density lipoproteins from embryonic cerebrospinal fluid modulates their roles in early neurogenesis.
    Caprile T
    Frontiers in neuroanatomy 9. (2015): 72.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    The inner lining of the reptilian brain: a heterogeneous cellular mosaic.
    Russo RE
    Glia 62.2 (2014 Feb): 300-16.

    The structural role of radial glial endfeet in confining spinal motor neuron somata is controlled by the Reelin and Notch pathways.
    Song MR
    Experimental neurology 249. (2013 Nov): 83-94.

    SCO-spondin from embryonic cerebrospinal fluid is required for neurogenesis during early brain development.
    Caprile T
    Frontiers in cellular neuroscience 7. (2013): 80.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    Regrowth of transected retinal ganglion cell axons despite persistent astrogliosis in the lizard (Gallotia galloti).
    Yanes CM
    Journal of anatomy 223.1 (2013 Jul): 22-37.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    WB References

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    IHC References
    All References

    Embryonic Origin of the Islet1 and Pax6 Neurons of the Chicken Central Extended Amygdala Using Cell Migration Assays and Relation to Different Neuropeptide-Containing Cells.
    Medina L
    Brain, behavior and evolution 85.3 (2015): 139-69.

    Combinatorial expression of Lef1, Lhx2, Lhx5, Lhx9, Lmo3, Lmo4, and Prox1 helps to identify comparable subdivisions in the developing hippocampal formation of mouse and chicken.
    Medina L
    Frontiers in neuroanatomy 8. (2014): 59.

    Calbindin expression in developing striatum of zebra finches and its relation to the formation of area X.
    Scharff C
    The Journal of comparative neurology 521.2 (2013 Feb 1): 326-41.

    Regrowth of transected retinal ganglion cell axons despite persistent astrogliosis in the lizard (Gallotia galloti).
    Yanes CM
    Journal of anatomy 223.1 (2013 Jul): 22-37.

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3.
    Bogerd J
    Endocrinology 156.10 (2015 Oct): 3804-17.

    Interaction between SCO-spondin and low density lipoproteins from embryonic cerebrospinal fluid modulates their roles in early neurogenesis.
    Caprile T
    Frontiers in neuroanatomy 9. (2015): 72.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    The inner lining of the reptilian brain: a heterogeneous cellular mosaic.
    Russo RE
    Glia 62.2 (2014 Feb): 300-16.

    The structural role of radial glial endfeet in confining spinal motor neuron somata is controlled by the Reelin and Notch pathways.
    Song MR
    Experimental neurology 249. (2013 Nov): 83-94.

    SCO-spondin from embryonic cerebrospinal fluid is required for neurogenesis during early brain development.
    Caprile T
    Frontiers in cellular neuroscience 7. (2013): 80.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

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