H5

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: H5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: VIM
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528506 
Uniprot ID: P09654 
RRID:  
Entrez Gene ID: 420519 
Clonality: Monoclonal
Immunogen: Chick optic tectum homogenate (E10)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Fox NY
Epitope Mapped: No
Antigen Name: Vimentin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/28/1994
Antigen Molecular Weight: Predicted: 53 kDa; Apparent: 53 kDa
Depositor: Sanes, J.R.
Antigen Sequence:
Depositor Institution: Harvard University
Antigen Species: Chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Chicken, Lizard, Mouse, Planaria, Turtle, Zebrafish
Additional Information: Vimentin is a radial glial cell marker.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    IF References

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3.
    Bogerd J
    Endocrinology 156.10 (2015 Oct): 3804-17.

    Interaction between SCO-spondin and low density lipoproteins from embryonic cerebrospinal fluid modulates their roles in early neurogenesis.
    Caprile T
    Frontiers in neuroanatomy 9. (2015): 72.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    The inner lining of the reptilian brain: a heterogeneous cellular mosaic.
    Russo RE
    Glia 62.2 (2014 Feb): 300-16.

    The structural role of radial glial endfeet in confining spinal motor neuron somata is controlled by the Reelin and Notch pathways.
    Song MR
    Experimental neurology 249. (2013 Nov): 83-94.

    SCO-spondin from embryonic cerebrospinal fluid is required for neurogenesis during early brain development.
    Caprile T
    Frontiers in cellular neuroscience 7. (2013): 80.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    Regrowth of transected retinal ganglion cell axons despite persistent astrogliosis in the lizard (Gallotia galloti).
    Yanes CM
    Journal of anatomy 223.1 (2013 Jul): 22-37.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

    WB References

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    IHC References
    FFPE References



    . (): .

    All References

    Embryonic Origin of the Islet1 and Pax6 Neurons of the Chicken Central Extended Amygdala Using Cell Migration Assays and Relation to Different Neuropeptide-Containing Cells.
    Medina L
    Brain, behavior and evolution 85.3 (2015): 139-69.

    Combinatorial expression of Lef1, Lhx2, Lhx5, Lhx9, Lmo3, Lmo4, and Prox1 helps to identify comparable subdivisions in the developing hippocampal formation of mouse and chicken.
    Medina L
    Frontiers in neuroanatomy 8. (2014): 59.

    Calbindin expression in developing striatum of zebra finches and its relation to the formation of area X.
    Scharff C
    The Journal of comparative neurology 521.2 (2013 Feb 1): 326-41.

    Regrowth of transected retinal ganglion cell axons despite persistent astrogliosis in the lizard (Gallotia galloti).
    Yanes CM
    Journal of anatomy 223.1 (2013 Jul): 22-37.



    . (): .

    Developmentally regulated and spatially restricted antigens of radial glial cells.
    Sanes JR
    Developmental dynamics : an official publication of the American Association of Anatomists 197.4 (1993 Aug): 307-18.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3.
    Bogerd J
    Endocrinology 156.10 (2015 Oct): 3804-17.

    Interaction between SCO-spondin and low density lipoproteins from embryonic cerebrospinal fluid modulates their roles in early neurogenesis.
    Caprile T
    Frontiers in neuroanatomy 9. (2015): 72.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    The inner lining of the reptilian brain: a heterogeneous cellular mosaic.
    Russo RE
    Glia 62.2 (2014 Feb): 300-16.

    The structural role of radial glial endfeet in confining spinal motor neuron somata is controlled by the Reelin and Notch pathways.
    Song MR
    Experimental neurology 249. (2013 Nov): 83-94.

    SCO-spondin from embryonic cerebrospinal fluid is required for neurogenesis during early brain development.
    Caprile T
    Frontiers in cellular neuroscience 7. (2013): 80.

    Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
    Yanes C
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2163-84.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

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