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SKU: P1A12
View product citations for antibody P1A12 on CiteAb

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Available: 141

DSHB Data Sheet

Catalog Fields

Antigen: glutathione-S-transferase (GST)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Linial, M.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human, Protein tag
Depositors Notes:
Antigen Molecular Weight: Predicted: 22 to 29 kDa (multiple genes encode different GST isoforms)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GSTP1
Immunogen: Recombinant Human GST
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: P09211 
Immunogen Sequence: Full length protein
Entrez Gene ID: 2950 
Additional Characterization:  
Antibody Registry ID: AB_2619603 
Additional Information: The Uniprot ID, gene ID and specific gene name provided may be different from the immunogen used. GSTs are encoded by multiple genes.
Recommended Applications: FACS, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1A12 was deposited to the DSHB by Wayner, E.A. / Linial, M. (DSHB Hybridoma Product P1A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
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  • Initial Publication
    WB References
    All References

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