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SKU: P1A12
View product citations for antibody P1A12 on CiteAb

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Available: 154

DSHB Data Sheet

Catalog Fields

Antigen: glutathione-S-transferase (GST)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Linial, M.
Isotype: MIgG2b
Depositors Institution: Fred Hutchison Cancer Research Center
Host Species: mouse
Gene: GSTP1
Positive Tested Species Reactivity: Human, Protein tag
Clonality: Monoclonal
Antigen Molecular Weight: Predicted: 22 to 29 kDa (multiple genes encode different GST isoforms)
Epitope Mapped: No
Immunogen: Recombinant Human GST
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Additional Information: The Uniprot ID, gene ID and specific gene name provided may be different from the immunogen used. GSTs are encoded by multiple genes.
Uniprot ID: P09211 
Entrez Gene ID: 2950 
Antibody Registry ID: AB_2619603 
Recommended Applications: FACS, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1A12 was deposited to the DSHB by Wayner, E.A. / Linial, M. (DSHB Hybridoma Product P1A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
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  • Initial Publication
    WB References
    All References

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