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$45.00 to $425.00
SKU: P1A12
View product citations for antibody P1A12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: glutathione-S-transferase (GST)
Available to For-Profits: Yes
Antigen Species: Schistosoma japonicum
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Wayner, E.A. / Linial, M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Protein tag
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: 26 kDa
Depositors Notes: GST fusion tag isthe 26-kDa glutathione-S-transferase protein from Schistosoma japonicum (blood fluke) . See PMID: 3047011. The DNA sequence for this 211 amino acid sequence may be integrated into expression vectors to generate recombinant fusion proteins.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant GST
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID: 24593871 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2619603 
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1A12 was deposited to the DSHB by Wayner, E.A. / Linial, M. (DSHB Hybridoma Product P1A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References
    All References

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