p1D9 (anti-rho1)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: p1D9 (anti-rho1)
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: Rho1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528263 
Uniprot ID: A4UZI6 
RRID:  
Entrez Gene ID: 36775 
Clonality: Monoclonal
Immunogen: Rho 1 cDNA/GST fusion protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: p1D9-A8
Epitope Mapped: No
Antigen Name: rho1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/9/2002
Antigen Molecular Weight: 21.7 kDa
Depositor: Parkhurst, S.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Drosophila
Depositor Notes: This antigen is also known as Ras-like GTP-binding protein Rho1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_528263 FBgn0014020
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p1D9 (anti-rho1) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p1D9 (anti-rho1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.
    Parkhurst SM
    Development (Cambridge, England) 129.16 (2002 Aug): 3771-82.

    Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner.
    Vale RD
    Current biology : CB 14.20 (2004 Oct 26): 1827-33.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Rho1 regulates signaling events required for proper Drosophila embryonic development.
    Parkhurst SM
    Developmental biology 278.1 (2005 Feb 1): 144-54.

    Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
    Engel JN
    Cellular microbiology 7.5 (2005 May): 725-39.

    Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.
    McKim KS
    Genetics 202.1 (2016 Jan): 61-75.

    RhoA GTPase inhibition organizes contraction during epithelial morphogenesis.
    Martin AC
    The Journal of cell biology 214.5 (2016 Aug 29): 603-17.

    Determinants of myosin II cortical localization during cytokinesis.
    Griffis ER
    Current biology : CB 20.12 (2010 Jun 22): 1080-5.

    Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.
    Lehmann R
    The Journal of cell biology 216.9 (2017 Sep 4): 2945-2958.

    Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis.
    Eggert US
    Nature chemical biology 6.6 (2010 Jun): 457-63.

    Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells.
    Buszczak M
    PLoS genetics 10.3 (2014 Mar): e1004200.

    WB References
    IHC References

    Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis.
    Jacinto A
    Development (Cambridge, England) 133.21 (2006 Nov): 4257-67.

    IP References
    All References

    Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis.
    Jacinto A
    Development (Cambridge, England) 133.21 (2006 Nov): 4257-67.

    Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.
    Parkhurst SM
    Development (Cambridge, England) 129.16 (2002 Aug): 3771-82.

    Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner.
    Vale RD
    Current biology : CB 14.20 (2004 Oct 26): 1827-33.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Rho1 regulates signaling events required for proper Drosophila embryonic development.
    Parkhurst SM
    Developmental biology 278.1 (2005 Feb 1): 144-54.

    Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
    Engel JN
    Cellular microbiology 7.5 (2005 May): 725-39.

    Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.
    McKim KS
    Genetics 202.1 (2016 Jan): 61-75.

    RhoA GTPase inhibition organizes contraction during epithelial morphogenesis.
    Martin AC
    The Journal of cell biology 214.5 (2016 Aug 29): 603-17.

    Determinants of myosin II cortical localization during cytokinesis.
    Griffis ER
    Current biology : CB 20.12 (2010 Jun 22): 1080-5.

    Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.
    Lehmann R
    The Journal of cell biology 216.9 (2017 Sep 4): 2945-2958.

    Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis.
    Eggert US
    Nature chemical biology 6.6 (2010 Jun): 457-63.

    Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells.
    Buszczak M
    PLoS genetics 10.3 (2014 Mar): e1004200.

    Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.
    Parkhurst SM
    Molecular biology of the cell 26.9 (2015 May 1): 1665-74.

    A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila.
    Ward RE
    PloS one 4.10 (2009 Oct 23): e7574.

    A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

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