p1D9 (anti-rho1)
Catalog Fields
Clone ID/Product Name: p1D9 (anti-rho1)
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Rho1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 36775
Clonality: Monoclonal
Immunogen: Rho 1 cDNA/GST fusion protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: p1D9-A8
Epitope Mapped: No
Antigen Name: rho1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/9/2002
Antigen Molecular Weight: 21.7 kDa
Depositor: Parkhurst, S.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Drosophila
Depositor Notes: This antigen is also known as Ras-like GTP-binding protein Rho1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_528263 FBgn0014020
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p1D9 (anti-rho1) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p1D9 (anti-rho1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.
Parkhurst SM
Development (Cambridge, England) 129.16 (2002 Aug): 3771-82.