p1D9 (anti-rho1)

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$40.00
SKU: p1D9 (anti-rho1)
View product citations for antibody p1D9 (anti-rho1) on CiteAb

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Available: 188

DSHB Data Sheet

Catalog Fields

Antigen: rho1
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Parkhurst, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is also known as Ras-like GTP-binding protein Rho1.
Antigen Molecular Weight: 21.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rho1
Immunogen: Rho 1 cDNA/GST fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: p1D9-A8
Epitope Location or Sequence:
Uniprot ID: A4UZI6 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 36775 
Additional Characterization:  
Antibody Registry ID: AB_528263 
Additional Information: RRID:AB_528263 FBgn0014020
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p1D9 (anti-rho1) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p1D9 (anti-rho1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.
    Parkhurst SM
    Development (Cambridge, England) 129.16 (2002 Aug): 3771-82.

    Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner.
    Vale RD
    Current biology : CB 14.20 (2004 Oct 26): 1827-33.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Rho1 regulates signaling events required for proper Drosophila embryonic development.
    Parkhurst SM
    Developmental biology 278.1 (2005 Feb 1): 144-54.

    Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
    Engel JN
    Cellular microbiology 7.5 (2005 May): 725-39.

    Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.
    McKim KS
    Genetics 202.1 (2016 Jan): 61-75.

    RhoA GTPase inhibition organizes contraction during epithelial morphogenesis.
    Martin AC
    The Journal of cell biology 214.5 (2016 Aug 29): 603-17.

    Determinants of myosin II cortical localization during cytokinesis.
    Griffis ER
    Current biology : CB 20.12 (2010 Jun 22): 1080-5.

    Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.
    Lehmann R
    The Journal of cell biology 216.9 (2017 Sep 4): 2945-2958.

    Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis.
    Eggert US
    Nature chemical biology 6.6 (2010 Jun): 457-63.

    Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells.
    Buszczak M
    PLoS genetics 10.3 (2014 Mar): e1004200.

    WB References
    IHC References

    Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis.
    Jacinto A
    Development (Cambridge, England) 133.21 (2006 Nov): 4257-67.

    IP References
    All References

    Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis.
    Jacinto A
    Development (Cambridge, England) 133.21 (2006 Nov): 4257-67.

    Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.
    Parkhurst SM
    Development (Cambridge, England) 129.16 (2002 Aug): 3771-82.

    Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner.
    Vale RD
    Current biology : CB 14.20 (2004 Oct 26): 1827-33.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Rho1 regulates signaling events required for proper Drosophila embryonic development.
    Parkhurst SM
    Developmental biology 278.1 (2005 Feb 1): 144-54.

    Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
    Engel JN
    Cellular microbiology 7.5 (2005 May): 725-39.

    Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.
    McKim KS
    Genetics 202.1 (2016 Jan): 61-75.

    RhoA GTPase inhibition organizes contraction during epithelial morphogenesis.
    Martin AC
    The Journal of cell biology 214.5 (2016 Aug 29): 603-17.

    Determinants of myosin II cortical localization during cytokinesis.
    Griffis ER
    Current biology : CB 20.12 (2010 Jun 22): 1080-5.

    Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.
    Lehmann R
    The Journal of cell biology 216.9 (2017 Sep 4): 2945-2958.

    Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis.
    Eggert US
    Nature chemical biology 6.6 (2010 Jun): 457-63.

    Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells.
    Buszczak M
    PLoS genetics 10.3 (2014 Mar): e1004200.

    Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.
    Parkhurst SM
    Molecular biology of the cell 26.9 (2015 May 1): 1665-74.

    A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila.
    Ward RE
    PloS one 4.10 (2009 Oct 23): e7574.

    A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

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