RT97

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$40.00
SKU: RT97
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DSHB Data Sheet

Catalog Fields

Antigen: neurofilament (NF-H)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Wood, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Sandoz Institute for Medical Research
Positive Tested Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Depositors Notes: The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).
Antigen Molecular Weight: Predicted: 112 kDa; Apparent: 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NEFH
Immunogen: Semi-purified neurofilaments from rat brain homogenate.
Alternate Gene Names: KIAA0845, NFH
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.
Uniprot ID: F1ND22 
Immunogen Sequence: Full length protein
Entrez Gene ID: 417020 
Additional Characterization:
Antibody Registry ID: AB_528399 
Additional Information: RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RT97 was deposited to the DSHB by Wood, J. (DSHB Hybridoma Product RT97)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Monoclonal antibodies to mammalian neurofilaments.
    Anderton BH
    Bioscience reports 1.3 (1981 Mar): 263-8.

    IF References

    Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival.
    Taylor JS
    Journal of neurobiology 63.1 (2005 Apr): 29-48.

    Retinal anatomy and visual performance in a diurnal cone-rich laboratory rodent, the Nile grass rat (Arvicanthis niloticus).
    Sauvé Y
    The Journal of comparative neurology 510.5 (2008 Oct 10): 525-38.

    Inner retina remodeling in a mouse model of stargardt-like macular dystrophy (STGD3).
    Sauvé Y
    Investigative ophthalmology and visual science 51.4 (2010 Apr): 2248-62.

    Pentoxifylline promotes recovery of erectile function in a rat model of postprostatectomy erectile dysfunction.
    Lue TF
    European urology 59.2 (2011 Feb): 286-96.

    Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases.
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    Human iPSC-Derived Neural Progenitors Preserve Vision in an AMD-Like Model.
    Wang S
    Stem cells (Dayton, Ohio) 33.8 (2015 Aug): 2537-49.

    Differential modulation of the juxtaparanodal complex in Multiple Sclerosis.
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    Matrix metalloproteinases in the mouse retina: a comparative study of expression patterns and MMP antibodies.
    Moons L
    BMC ophthalmology 15. (2015 Dec 29): 187.

    Cutaneous inflammation differentially regulates the expression and function of Angiotensin-II types 1 and 2 receptors in rat primary sensory neurons.
    Acosta CG
    Journal of neurochemistry . (2019 Aug 6): .

    Ectopic expression of L1CAM ectodomain alters differentiation and motility, but not proliferation, of human neural progenitor cells.
    Galileo DS
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 78. (2019 Nov): 49-64.

    WB References
    IHC References

    Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants.
    Kahn J
    Nature 298.5869 (1982 Jul 1): 84-6.

    The molecular composition of the extracellular matrix of the human iliolumbar ligament.
    Milz S
    The spine journal : official journal of the North American Spine Society 15.6 (2015 Jun 1): 1325-31.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Afferent-inherent regulation of myosin heavy chain isoforms in rat muscle spindles.
    Story GM
    Muscle and nerve 20.12 (1997 Dec): 1549-60.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Neurofilament expression in human T lymphocytes.
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    E6020, a synthetic TLR4 agonist, accelerates myelin debris clearance, Schwann cell infiltration, and remyelination in the rat spinal cord.
    McTigue DM
    Glia 65.6 (2017 Jun): 883-899.

    FACS References

    Neurofilament expression in human T lymphocytes.
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    Epitope Map References
    All References

    Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants.
    Kahn J
    Nature 298.5869 (1982 Jul 1): 84-6.

    The molecular composition of the extracellular matrix of the human iliolumbar ligament.
    Milz S
    The spine journal : official journal of the North American Spine Society 15.6 (2015 Jun 1): 1325-31.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Afferent-inherent regulation of myosin heavy chain isoforms in rat muscle spindles.
    Story GM
    Muscle and nerve 20.12 (1997 Dec): 1549-60.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Neurofilament expression in human T lymphocytes.
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    E6020, a synthetic TLR4 agonist, accelerates myelin debris clearance, Schwann cell infiltration, and remyelination in the rat spinal cord.
    McTigue DM
    Glia 65.6 (2017 Jun): 883-899.

    Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival.
    Taylor JS
    Journal of neurobiology 63.1 (2005 Apr): 29-48.

    Retinal anatomy and visual performance in a diurnal cone-rich laboratory rodent, the Nile grass rat (Arvicanthis niloticus).
    Sauvé Y
    The Journal of comparative neurology 510.5 (2008 Oct 10): 525-38.

    Inner retina remodeling in a mouse model of stargardt-like macular dystrophy (STGD3).
    Sauvé Y
    Investigative ophthalmology and visual science 51.4 (2010 Apr): 2248-62.

    Pentoxifylline promotes recovery of erectile function in a rat model of postprostatectomy erectile dysfunction.
    Lue TF
    European urology 59.2 (2011 Feb): 286-96.

    Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases.
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    Human iPSC-Derived Neural Progenitors Preserve Vision in an AMD-Like Model.
    Wang S
    Stem cells (Dayton, Ohio) 33.8 (2015 Aug): 2537-49.

    Differential modulation of the juxtaparanodal complex in Multiple Sclerosis.
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    Matrix metalloproteinases in the mouse retina: a comparative study of expression patterns and MMP antibodies.
    Moons L
    BMC ophthalmology 15. (2015 Dec 29): 187.

    Cutaneous inflammation differentially regulates the expression and function of Angiotensin-II types 1 and 2 receptors in rat primary sensory neurons.
    Acosta CG
    Journal of neurochemistry . (2019 Aug 6): .

    Ectopic expression of L1CAM ectodomain alters differentiation and motility, but not proliferation, of human neural progenitor cells.
    Galileo DS
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 78. (2019 Nov): 49-64.

    Alzheimer's paired helical filaments share epitopes with neurofilament side arms.
    Probst A
    The EMBO journal 5.2 (1986 Feb): 269-76.

    Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia.
    Won MH
    Brain research 1016.1 (2004 Jul 30): 119-28.

    Synaptophysin depletion and intraneuronal Aβ in organotypic hippocampal slice cultures from huAPP transgenic mice.
    Coleman MP
    Molecular neurodegeneration 11.1 (2016 Jun 10): 44.

    Monoclonal antibodies to mammalian neurofilaments.
    Anderton BH
    Bioscience reports 1.3 (1981 Mar): 263-8.

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