RT97
Catalog Fields
Antigen: neurofilament (NF-H)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Wood, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Sandoz Institute for Medical Research
Positive Tested Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Depositors Notes: The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).
Antigen Molecular Weight: Predicted: 112 kDa; Apparent: 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: NEFH
Immunogen: Semi-purified neurofilaments from rat brain homogenate.
Alternate Gene Names: KIAA0845, NFH
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.
Uniprot ID: F1ND22
Immunogen Sequence: Full length protein
Entrez Gene ID: 417020
Additional Characterization:
Antibody Registry ID: AB_528399
Additional Information: RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RT97 was deposited to the DSHB by Wood, J. (DSHB Hybridoma Product RT97)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Monoclonal antibodies to mammalian neurofilaments.
Anderton BH
Bioscience reports 1.3 (1981 Mar): 263-8.