RT97

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$40.00
SKU: RT97
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DSHB Data Sheet

Catalog Fields

Antigen: neurofilament (NF-H)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Wood, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Sandoz Institute for Medical Research
Positive Tested Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Depositors Notes: The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).
Antigen Molecular Weight: Predicted: 112 kDa; Apparent: 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NEFH
Immunogen: Semi-purified neurofilaments from rat brain homogenate.
Alternate Gene Names: KIAA0845, NFH
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.
Uniprot ID: F1ND22 
Immunogen Sequence: Full length protein
Entrez Gene ID: 417020 
Additional Characterization:
Antibody Registry ID: AB_528399 
Additional Information: RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RT97 was deposited to the DSHB by Wood, J. (DSHB Hybridoma Product RT97)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Bioscience reports
    Anderton BH
    Bioscience reports 1.3 (1981 Mar): 263-8.

    IF References

    Journal of neurobiology
    Taylor JS
    Journal of neurobiology 63.1 (2005 Apr): 29-48.

    The Journal of comparative neurology
    Sauvé Y
    The Journal of comparative neurology 510.5 (2008 Oct 10): 525-38.

    Investigative ophthalmology & visual science
    Sauvé Y
    Investigative ophthalmology & visual science 51.4 (2010 Apr): 2248-62.

    European urology
    Lue TF
    European urology 59.2 (2011 Feb): 286-96.

    Journal of neurochemistry
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    Stem cells (Dayton, Ohio)
    Wang S
    Stem cells (Dayton, Ohio) 33.8 (2015 Aug): 2537-49.

    Molecular and cellular neurosciences
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    BMC ophthalmology
    Moons L
    BMC ophthalmology 15. (2015 Dec 29): 187.

    Journal of neurochemistry
    Acosta CG
    Journal of neurochemistry . (2019 Aug 6): .

    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
    Galileo DS
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 78. (2019 Nov): 49-64.

    WB References

    Nature
    Kahn J
    Nature 298.5869 (1982 Jul 1): 84-6.

    Journal of neurobiology
    Taylor JS
    Journal of neurobiology 63.1 (2005 Apr): 29-48.

    Journal of neurochemistry
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    The EMBO journal
    Probst A
    The EMBO journal 5.2 (1986 Feb): 269-76.

    Brain research
    Won MH
    Brain research 1016.1 (2004 Jul 30): 119-28.

    Molecular neurodegeneration
    Coleman MP
    Molecular neurodegeneration 11.1 (2016 Jun 10): 44.

    Immunology
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    IHC References

    Nature
    Kahn J
    Nature 298.5869 (1982 Jul 1): 84-6.

    The spine journal : official journal of the North American Spine Society
    Milz S
    The spine journal : official journal of the North American Spine Society 15.6 (2015 Jun 1): 1325-31.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Muscle & nerve
    Story GM
    Muscle & nerve 20.12 (1997 Dec): 1549-60.

    The Anatomical record
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Immunology
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    Glia
    McTigue DM
    Glia 65.6 (2017 Jun): 883-899.

    FACS References

    Immunology
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    Epitope Map References

    Journal of neurochemistry
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    All References

    Nature
    Kahn J
    Nature 298.5869 (1982 Jul 1): 84-6.

    The spine journal : official journal of the North American Spine Society
    Milz S
    The spine journal : official journal of the North American Spine Society 15.6 (2015 Jun 1): 1325-31.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Muscle & nerve
    Story GM
    Muscle & nerve 20.12 (1997 Dec): 1549-60.

    The Anatomical record
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Immunology
    Kelleher D
    Immunology 79.1 (1993 May): 167-70.

    Glia
    McTigue DM
    Glia 65.6 (2017 Jun): 883-899.

    Journal of neurobiology
    Taylor JS
    Journal of neurobiology 63.1 (2005 Apr): 29-48.

    The Journal of comparative neurology
    Sauvé Y
    The Journal of comparative neurology 510.5 (2008 Oct 10): 525-38.

    Investigative ophthalmology & visual science
    Sauvé Y
    Investigative ophthalmology & visual science 51.4 (2010 Apr): 2248-62.

    European urology
    Lue TF
    European urology 59.2 (2011 Feb): 286-96.

    Journal of neurochemistry
    Nixon RA
    Journal of neurochemistry 107.1 (2008 Oct): 35-49.

    Stem cells (Dayton, Ohio)
    Wang S
    Stem cells (Dayton, Ohio) 33.8 (2015 Aug): 2537-49.

    Molecular and cellular neurosciences
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    BMC ophthalmology
    Moons L
    BMC ophthalmology 15. (2015 Dec 29): 187.

    Journal of neurochemistry
    Acosta CG
    Journal of neurochemistry . (2019 Aug 6): .

    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
    Galileo DS
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 78. (2019 Nov): 49-64.

    The EMBO journal
    Probst A
    The EMBO journal 5.2 (1986 Feb): 269-76.

    Brain research
    Won MH
    Brain research 1016.1 (2004 Jul 30): 119-28.

    Molecular neurodegeneration
    Coleman MP
    Molecular neurodegeneration 11.1 (2016 Jun 10): 44.

    Bioscience reports
    Anderton BH
    Bioscience reports 1.3 (1981 Mar): 263-8.

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