Antibody Name: RT97
Antigen:neurofilament (NF-H)
Catalog Fields
Identity
Clone ID / Antibody Name: RT97
Alternate Antibody Name: —
Antigen Name: neurofilament (NF-H)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: NEFH
Entrez Gene ID: 417020
Antigen Sequence: —
Antigen Species: Rat
Antigen Mol. Weight: Predicted: 112 kDa; Apparent: 200 kDa
Uniprot ID: F1ND22
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: X63.Ag8.653
Antibody Registry / RRID: AB_528399
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Semi-purified neurofilaments from rat brain homogenate.
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.
Clone: —
Additional Info: RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Bovine, Chicken, Human, Mouse, Rat
Predicted Reactivity: —
Depositor
Depositor Name: J. Wood
Institution: Sandoz Institute for Medical Research
Deposit Date: July 30, 1987
Depositor Notes: The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).
Recommended Applications
FACS
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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