RT97
Catalog Fields
Antigen: neurofilament (NF-H)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Wood, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Sandoz Institute for Medical Research
Positive Tested Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Depositors Notes: The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).
Predicted Species Reactivity:
Gene: NEFH
Immunogen: Semi-purified neurofilaments from rat brain homogenate.
Alternate Gene Names: KIAA0845, NFH
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.
Uniprot ID: F1ND22
Immunogen Sequence: Full length protein
Entrez Gene ID: 417020
Antibody Registry ID: AB_528399
Additional Information: RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RT97 was deposited to the DSHB by Wood, J. (DSHB Hybridoma Product RT97)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
23 References
Initial Publication
Monoclonal antibodies to mammalian neurofilaments.
Anderton BH
Bioscience reports 1.3 (1981 Mar): 263-8.