Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Hiromi, Y. / Hondo, T. / Kanda, H.
Host Species: mouse
Depositors Institution: Keio University School of Medicine
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Seven-up is the highly conserved Drosophila homolog of the human TF-COUP2,a nuclear receptor. The Drosophila protein is over 90% identical to the human homolog.
Predicted Species Reactivity:
Immunogen: Recombinant peptide
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Epitope Location or Sequence: a.a. 25-40
Uniprot ID: P16376
Immunogen Sequence: N-terminal region including DNA binding domain
Entrez Gene ID: 41491
Antibody Registry ID: AB_2618079
Additional Information: RRID:AB_2618079
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Seven-up 2D3 was deposited to the DSHB by Hiromi, Y. / Hondo, T. / Kanda, H. (DSHB Hybridoma Product Seven-up 2D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.