anti-Shot mAbRod1

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$45.00 to $425.00
SKU: anti-Shot mAbRod1
View product citations for antibody anti-Shot mAbRod1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Shot
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Kolodziej, P.A.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University Medical Center
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Shot colocalizes with adherens junctions of tracheal cells and of chordotonal neurons and their support cells.
Antigen Molecular Weight: Mutiple isoforms; Predicted: from 465 to 989 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: shot
Immunogen: Recombinant GST-Shot/Rod domain (aa 1454-1909 from isoform A)
Alternate Gene Names: CG18076, kak, l(2)k04204, Grv, kopshortstop, kakapo
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 1454-1909 from isoform A
Uniprot ID: A1Z9J1 
Immunogen Sequence: Partial protein
Entrez Gene ID: 36542 
Additional Characterization:  
Antibody Registry ID: AB_528467 
Additional Information: shot: short stop. Should recognize all known isoforms except isoforms C, E, H, and Q that may not be recognized.
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Shot mAbRod1 was deposited to the DSHB by Kolodziej, P.A. (DSHB Hybridoma Product anti-Shot mAbRod1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.
    Kolodziej PA
    Development (Cambridge, England) 130.24 (2003 Dec): 5989-99.

    IF References
    IHC References
    Epitope Map References

    Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.
    Kolodziej PA
    Development (Cambridge, England) 130.24 (2003 Dec): 5989-99.

    All References

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