anti-Shot mAbRod1

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SKU: anti-Shot mAbRod1
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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-Shot mAbRod1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: shot
Ab Isotype: MIgG2b, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528467 
Uniprot ID: A1Z9J1 
Entrez Gene ID: 36542 
Clonality: Monoclonal
Immunogen: Recombinant GST-Shot/Rod domain (aa 1454-1909 from isoform A)
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Shot
Epitope Location or Sequence: aa 1454-1909 from isoform A
Alternate Antigen Name:
Deposit Date: 10/12/2005
Antigen Molecular Weight: Mutiple isoforms; Predicted: from 465 to 989 kDa
Depositor: Kolodziej, P.A.
Antigen Sequence:
Depositor Institution: Vanderbilt University Medical Center
Antigen Species: Drosophila
Depositor Notes: Shot colocalizes with adherens junctions of tracheal cells and of chordotonal neurons and their support cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: shot: short stop. Should recognize all known isoforms except isoforms C, E, H, and Q that may not be recognized.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Shot mAbRod1 was deposited to the DSHB by Kolodziej, P.A. (DSHB Hybridoma Product anti-Shot mAbRod1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.
    Kolodziej PA
    Development (Cambridge, England) 130.24 (2003 Dec): 5989-99.

    IF References
    IHC References
    Epitope Map References

    Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.
    Kolodziej PA
    Development (Cambridge, England) 130.24 (2003 Dec): 5989-99.

    All References

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