anti-Yan 8B12H9

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$45.00 to $425.00
SKU: anti-Yan 8B12H9
View product citations for antibody anti-Yan 8B12H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Yan Drosophila protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Rubin, G.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of California, Berkeley
Antigen Molecular Weight: Predicted: 79 kDa
Depositors Notes: This antibody works well for immunocytochemistry in embryos, imaginal discs and cultured cells, immunoprecipitation and Western blotting. Freezing significantly reduces the effective titre – supernatants are stable for a minimum of 5 years at 4° C with no loss of titre.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant GST/Yan (aa 69-388) fusion protein
Gene: aop
Alternate Antibody Name: 8B12
Alternate Gene Names: CG3166, Yan, pok, DROYANETSB
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Uniprot ID: Q01842 
Epitope Location or Sequence: aa 69-388
Entrez Gene ID: 33392 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_531807 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: The Yan protein is also known as Ets DNA-binding protein pokkuri and anterior open.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Yan 8B12H9 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product anti-Yan 8B12H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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