anti-Yan 8B12H9

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SKU: anti-Yan 8B12H9
View product citations for antibody anti-Yan 8B12H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Yan Drosophila protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody works well for immunocytochemistry in embryos, imaginal discs and cultured cells, immunoprecipitation and Western blotting. Freezing significantly reduces the effective titre – supernatants are stable for a minimum of 5 years at 4° C with no loss of titre.
Antigen Molecular Weight: Predicted: 79 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: aop
Immunogen: Recombinant GST/Yan (aa 69-388) fusion protein
Alternate Gene Names: CG3166, Yan, pok, DROYANETSB
Alternate Antibody Name: 8B12
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: aa 69-388
Uniprot ID: Q01842 
Immunogen Sequence: Partial protein
Entrez Gene ID: 33392 
Additional Characterization:  
Antibody Registry ID: AB_531807 
Additional Information: The Yan protein is also known as Ets DNA-binding protein pokkuri and anterior open.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Yan 8B12H9 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product anti-Yan 8B12H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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