anti-Yan 8B12H9

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SKU: anti-Yan 8B12H9

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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-Yan 8B12H9
Available to For-Profits: Yes
Alternate Antibody Name: 8B12
Gene Name: aop
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531807 
Uniprot ID: Q01842 
RRID:  
Entrez Gene ID: 33392 
Clonality: Monoclonal
Immunogen: Recombinant GST/Yan (aa 69-388) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: Yan Drosophila protein
Epitope Location or Sequence: aa 69-388
Alternate Antigen Name:
Deposit Date: 2/16/2005
Antigen Molecular Weight: Predicted: 79 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This antibody works well for immunocytochemistry in embryos, imaginal discs and cultured cells, immunoprecipitation and Western blotting. Freezing significantly reduces the effective titre – supernatants are stable for a minimum of 5 years at 4° C with no loss of titre.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The Yan protein is also known as Ets DNA-binding protein pokkuri and anterior open.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Yan 8B12H9 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product anti-Yan 8B12H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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