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$45.00 to $425.00
SKU: anti-lozenge
View product citations for antibody anti-lozenge on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Lozenge
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Banerjee, U.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of California, Los Angeles
Antigen Molecular Weight: 84.7 kDa
Depositors Notes: The transcription factor lozenge shares a 71% identity with the human AML1 and has a role in Drosophila hematopoiesis.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Mixture of two lozenge C-terminus GST-fusion proteins
Gene: lz
Alternate Antibody Name:
Alternate Gene Names: Dmel_CG1689
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q9W349 
Epitope Location or Sequence:
Entrez Gene ID: 31883 
Immunogen Sequence: bp 1847-2474 & bp 2474-3471
Antibody Registry ID: AB_528346 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: RRID: AB_528346 FBgn0002576
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-lozenge was deposited to the DSHB by Banerjee, U. (DSHB Hybridoma Product anti-lozenge)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Specification of Drosophila hematopoietic lineage by conserved transcription factors.
    Banerjee U
    Science (New York, N.Y.) 288.5463 (2000 Apr 7): 146-9.

    IF References
    IHC References
    All References

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