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SKU: anti-lozenge
View product citations for antibody anti-lozenge on CiteAb

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Available: 118

DSHB Data Sheet

Catalog Fields

Antigen: Lozenge
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Banerjee, U.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California Los Angeles
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The transcription factor lozenge shares a 71% identity with the human AML1 and has a role in Drosophila hematopoiesis.
Antigen Molecular Weight: 84.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: lz
Immunogen: Mixture of two lozenge C-terminus GST-fusion proteins
Alternate Gene Names: Dmel_CG1689
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9W349 
Immunogen Sequence: bp 1847-2474 & bp 2474-3471
Entrez Gene ID: 31883 
Additional Characterization:  
Antibody Registry ID: AB_528346 
Additional Information: RRID: AB_528346 FBgn0002576
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-lozenge was deposited to the DSHB by Banerjee, U. (DSHB Hybridoma Product anti-lozenge)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Specification of Drosophila hematopoietic lineage by conserved transcription factors.
    Banerjee U
    Science (New York, N.Y.) 288.5463 (2000 Apr 7): 146-9.

    IF References
    IHC References
    All References

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