14A3D2

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SKU: 14A3D2

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 14A3D2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_579782 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Mmp1 hemopexin domain (recombinant)
Clone:
Immunogen Sequence:
Myeloma Strain: Designation of P3XAg8.653
Epitope Mapped: No
Antigen Name: Mmp1 C-terminus
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/2/2006
Antigen Molecular Weight:
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The amount of antigen used for immunization was SQ injection of 50 µg in RIBI adjuvant every 2-3 weeks. The route of immunization was IP 100 µg aqueous antigen 5 days prior to fusion. The origin of the species was E. coli. RRID:AB_579782
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14A3D2 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 14A3D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    IF References

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    IHC References
    All References

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death & disease 10.8 (2019 Aug 14): 613.

    The Upd3 cytokine couples inflammation to maturation defects in Drosophila.
    Milán M
    Current biology : CB 31.8 (2021 Apr 26): 1780-1787.e6.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

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