14A3D2

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$40.00
SKU: 14A3D2
View product citations for antibody 14A3D2 on CiteAb

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Available: 109

DSHB Data Sheet

Catalog Fields

Antigen: Mmp1 C-terminus
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California-Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Mmp1 hemopexin domain (recombinant)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of P3XAg8.653
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_579782 
Additional Information: The amount of antigen used for immunization was SQ injection of 50 µg in RIBI adjuvant every 2-3 weeks. The route of immunization was IP 100 µg aqueous antigen 5 days prior to fusion. The origin of the species was E. coli. RRID:AB_579782
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14A3D2 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 14A3D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    IF References

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    IHC References
    All References

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death and disease 10.8 (2019 Aug 14): 613.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

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