5H7B11
Catalog Fields
Antigen: Mmp1 catalytic domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Janelia Farm Research / HHMI
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: Mmp1
Immunogen: Mmp1 catalytic domain (amino acids 137-299 of Mmp1)-GST recombinant fusion protein
Alternate Gene Names: Mmp 1 MMP-1 mmp1 MMp1 MMP1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9W122
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Entrez Gene ID: 37949
Antibody Registry ID: AB_579779
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 3A6B4.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5H7B11 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 5H7B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
28 References
Initial Publication
An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
Page-McCaw A
Genes & development 20.14 (2006 Jul 15): 1899-910.