3B8D12
Catalog Fields
Antigen: Mmp1 catalytic domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Janelia Farm Research / HHMI
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Antigen Molecular Weight: Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Predicted Species Reactivity:
Gene: MMP1
Immunogen: GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Alternate Gene Names: dm1-MMP; Dm1-MMP; Dmel\CG4859; dmmp1; dMMP1;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9W122
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Entrez Gene ID: 37949
Antibody Registry ID: AB_579781
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B8D12 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3B8D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
25 References
Initial Publication
Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
Rubin GM
Developmental cell 4.1 (2003 Jan): 95-106.