3B8D12

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SKU: 3B8D12

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 3B8D12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MMP1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_579781 
Uniprot ID: Q9W122 
RRID:  
Entrez Gene ID: 37949 
Clonality: Monoclonal
Immunogen: GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Clone:
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Antigen Name: Mmp1 catalytic domain
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/2/2006
Antigen Molecular Weight: Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: Janelia Farm Research / HHMI
Antigen Species: Drosophila
Depositor Notes: This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B8D12 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3B8D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes & development 20.14 (2006 Jul 15): 1899-910.

    Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
    Rubin GM
    Developmental cell 4.1 (2003 Jan): 95-106.

    Drosophila actin-Capping Protein limits JNK activation by the Src proto-oncogene.
    Janody F
    Oncogene 33.16 (2014 Apr 17): 2027-39.

    A matrix metalloproteinase mediates airway remodeling in Drosophila.
    Page-McCaw A
    Developmental biology 344.2 (2010 Aug 15): 772-83.

    Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila.
    Zervas CG
    PloS one 7.10 (2012): e47355.

    Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.
    Murray MJ
    Nature communications 4. (2013): 2790.

    Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway.
    Moberg KH
    Cell cycle (Georgetown, Tex.) 10.23 (2011 Dec 1): 4110-8.

    In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
    Richardson HE
    Disease models & mechanisms 6.3 (2013 May): 661-78.

    A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis.
    Janody F
    Developmental biology 360.1 (2011 Dec 1): 143-59.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.
    Jiao R
    Development (Cambridge, England) 138.12 (2011 Jun): 2477-85.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila.
    Igaki T
    Developmental biology 395.1 (2014 Nov 1): 19-28.

    Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut.
    Yoo MA
    Experimental cell research 318.5 (2012 Mar 10): 670-81.

    The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor.
    Duman-Scheel M
    BMC developmental biology 11. (2011 Jun 14): 41.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development.
    Cohen SM
    Current biology : CB 24.13 (2014 Jul 7): 1476-84.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Dissemination of Ras(V12)-transformed cells requires the mechanosensitive channel Piezo.
    Kwon YV
    Nature communications 11.1 (2020 Jul 16): 3568.

    Novel function of N-acetyltransferase for microtubule stability and JNK signaling in Drosophila organ development.
    Choi KW
    Proceedings of the National Academy of Sciences of the United States of America 118.4 (2021 Jan 26): .

    WB References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes & development 20.14 (2006 Jul 15): 1899-910.

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    IHC References
    IP References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes & development 20.14 (2006 Jul 15): 1899-910.

    Epitope Map References
    All References

    Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo.
    Broihier HT
    Development (Cambridge, England) 135.1 (2008 Jan): 95-109.

    A novel injury paradigm in the central nervous system of adult Drosophila: molecular, cellular and functional aspects.
    Casas-Tintó S
    Disease models & mechanisms 14.5 (2021 May 1): .

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes & development 20.14 (2006 Jul 15): 1899-910.

    Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
    Rubin GM
    Developmental cell 4.1 (2003 Jan): 95-106.

    Drosophila actin-Capping Protein limits JNK activation by the Src proto-oncogene.
    Janody F
    Oncogene 33.16 (2014 Apr 17): 2027-39.

    A matrix metalloproteinase mediates airway remodeling in Drosophila.
    Page-McCaw A
    Developmental biology 344.2 (2010 Aug 15): 772-83.

    Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila.
    Zervas CG
    PloS one 7.10 (2012): e47355.

    Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.
    Murray MJ
    Nature communications 4. (2013): 2790.

    Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway.
    Moberg KH
    Cell cycle (Georgetown, Tex.) 10.23 (2011 Dec 1): 4110-8.

    In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
    Richardson HE
    Disease models & mechanisms 6.3 (2013 May): 661-78.

    A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis.
    Janody F
    Developmental biology 360.1 (2011 Dec 1): 143-59.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.
    Jiao R
    Development (Cambridge, England) 138.12 (2011 Jun): 2477-85.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila.
    Igaki T
    Developmental biology 395.1 (2014 Nov 1): 19-28.

    Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut.
    Yoo MA
    Experimental cell research 318.5 (2012 Mar 10): 670-81.

    The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor.
    Duman-Scheel M
    BMC developmental biology 11. (2011 Jun 14): 41.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development.
    Cohen SM
    Current biology : CB 24.13 (2014 Jul 7): 1476-84.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Dissemination of Ras(V12)-transformed cells requires the mechanosensitive channel Piezo.
    Kwon YV
    Nature communications 11.1 (2020 Jul 16): 3568.

    Novel function of N-acetyltransferase for microtubule stability and JNK signaling in Drosophila organ development.
    Choi KW
    Proceedings of the National Academy of Sciences of the United States of America 118.4 (2021 Jan 26): .

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    The Scribble module regulates retromer-dependent endocytic trafficking during epithelial polarization.
    Bilder D
    Development (Cambridge, England) 141.14 (2014 Jul): 2796-802.

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