3B8D12
Catalog Fields
Antigen: Mmp1 catalytic domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Depositors Institution: HHMI / Janelia Farm Research
Host Species: mouse
Depositors Notes: This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Positive Tested Species Reactivity: Drosophila
Gene: MMP1
Antigen Molecular Weight: Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Alternate Gene Names: dm1-MMP; Dm1-MMP; Dmel\CG4859; dmmp1; dMMP1;
Immunogen: GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Clonality: Monoclonal
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Uniprot ID: Q9W122
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Entrez Gene ID: 37949
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
Antibody Registry ID: AB_579781
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B8D12 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3B8D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
Rubin GM
Developmental cell 4.1 (2003 Jan): 95-106.