3B8D12
Catalog Fields
Antigen: Mmp1 catalytic domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Antigen Molecular Weight: Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: MMP1
Immunogen: GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Alternate Gene Names: dm1-MMP; Dm1-MMP; Dmel\CG4859; dmmp1; dMMP1;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9W122
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Entrez Gene ID: 37949
Additional Characterization:
Antibody Registry ID: AB_579781
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B8D12 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3B8D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
Rubin GM
Developmental cell 4.1 (2003 Jan): 95-106.