3B8D12

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$40.00
SKU: 3B8D12
View product citations for antibody 3B8D12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Mmp1 catalytic domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Antigen Molecular Weight: Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MMP1
Immunogen: GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Alternate Gene Names: dm1-MMP; Dm1-MMP; Dmel\CG4859; dmmp1; dMMP1;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9W122 
Immunogen Sequence: Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Entrez Gene ID: 37949 
Additional Characterization:
Antibody Registry ID: AB_579781 
Additional Information: This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B8D12 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3B8D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes and development 20.14 (2006 Jul 15): 1899-910.

    Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
    Rubin GM
    Developmental cell 4.1 (2003 Jan): 95-106.

    Drosophila actin-Capping Protein limits JNK activation by the Src proto-oncogene.
    Janody F
    Oncogene 33.16 (2014 Apr 17): 2027-39.

    A matrix metalloproteinase mediates airway remodeling in Drosophila.
    Page-McCaw A
    Developmental biology 344.2 (2010 Aug 15): 772-83.

    Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila.
    Zervas CG
    PloS one 7.10 (2012): e47355.

    Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.
    Murray MJ
    Nature communications 4. (2013): 2790.

    Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway.
    Moberg KH
    Cell cycle (Georgetown, Tex.) 10.23 (2011 Dec 1): 4110-8.

    In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
    Richardson HE
    Disease models and mechanisms 6.3 (2013 May): 661-78.

    A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis.
    Janody F
    Developmental biology 360.1 (2011 Dec 1): 143-59.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.
    Jiao R
    Development (Cambridge, England) 138.12 (2011 Jun): 2477-85.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila.
    Igaki T
    Developmental biology 395.1 (2014 Nov 1): 19-28.

    Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut.
    Yoo MA
    Experimental cell research 318.5 (2012 Mar 10): 670-81.

    The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor.
    Duman-Scheel M
    BMC developmental biology 11. (2011 Jun 14): 41.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development.
    Cohen SM
    Current biology : CB 24.13 (2014 Jul 7): 1476-84.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    WB References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes and development 20.14 (2006 Jul 15): 1899-910.

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    IHC References

    Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo.
    Broihier HT
    Development (Cambridge, England) 135.1 (2008 Jan): 95-109.

    IP References

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes and development 20.14 (2006 Jul 15): 1899-910.

    Epitope Map References
    All References

    Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo.
    Broihier HT
    Development (Cambridge, England) 135.1 (2008 Jan): 95-109.

    An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion.
    Page-McCaw A
    Genes and development 20.14 (2006 Jul 15): 1899-910.

    Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development.
    Rubin GM
    Developmental cell 4.1 (2003 Jan): 95-106.

    Drosophila actin-Capping Protein limits JNK activation by the Src proto-oncogene.
    Janody F
    Oncogene 33.16 (2014 Apr 17): 2027-39.

    A matrix metalloproteinase mediates airway remodeling in Drosophila.
    Page-McCaw A
    Developmental biology 344.2 (2010 Aug 15): 772-83.

    Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila.
    Zervas CG
    PloS one 7.10 (2012): e47355.

    Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.
    Murray MJ
    Nature communications 4. (2013): 2790.

    Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway.
    Moberg KH
    Cell cycle (Georgetown, Tex.) 10.23 (2011 Dec 1): 4110-8.

    In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
    Richardson HE
    Disease models and mechanisms 6.3 (2013 May): 661-78.

    A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis.
    Janody F
    Developmental biology 360.1 (2011 Dec 1): 143-59.

    Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
    Stenmark H
    PloS one 4.2 (2009): e4354.

    Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.
    Jiao R
    Development (Cambridge, England) 138.12 (2011 Jun): 2477-85.

    Epithelial junctions maintain tissue architecture by directing planar spindle orientation.
    Gibson MC
    Nature 500.7462 (2013 Aug 15): 359-62.

    A secreted MMP is required for reepithelialization during wound healing.
    Page-McCaw A
    Molecular biology of the cell 23.6 (2012 Mar): 1068-79.

    Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila.
    Igaki T
    Developmental biology 395.1 (2014 Nov 1): 19-28.

    Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut.
    Yoo MA
    Experimental cell research 318.5 (2012 Mar 10): 670-81.

    The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor.
    Duman-Scheel M
    BMC developmental biology 11. (2011 Jun 14): 41.

    Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
    Fehon RG
    The Journal of cell biology 189.2 (2010 Apr 19): 311-23.

    Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development.
    Cohen SM
    Current biology : CB 24.13 (2014 Jul 7): 1476-84.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
    Page-McCaw A
    Proceedings of the National Academy of Sciences of the United States of America 106.8 (2009 Feb 24): 2659-64.

    The Scribble module regulates retromer-dependent endocytic trafficking during epithelial polarization.
    Bilder D
    Development (Cambridge, England) 141.14 (2014 Jul): 2796-802.

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