21A6
Catalog Fields
Antigen: Eyes shut protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology, Division of Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The eyes shut protein antigen was originally identified as "Retinal Space (mechanoreceptors?)".
Predicted Species Reactivity:
Gene: EYS
Immunogen: Retinal homogenates
Alternate Gene Names: Spacemaker, spam, mAb 21A6
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: The epitope lies between the 5th and 7th EGF repeat at the N-terminus.
Uniprot ID: A0A1F4
Immunogen Sequence: Full length protein
Entrez Gene ID: 3771890
Antibody Registry ID: AB_528449
Additional Information: The immunization was 5 injections containing material from 20-50 flies through 4 intraperitoneal routes and 1 intravenous injection. The origin of the immunogen is C-S strain. For immunohistochemistry, cryostat sections fixed in 2% formalin for 30 minutes is recommended. RRID:AB_528449. FBgn0031414
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
21A6 was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product 21A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
27 References
Initial Publication
Monoclonal antibodies against the Drosophila nervous system.
Shotwell SL
Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.