21A6

(0) No Reviews yet
$40.00
SKU: 21A6
View product citations for antibody 21A6 on CiteAb

In Stock

Available: 161

DSHB Data Sheet

Catalog Fields

Antigen: Eyes shut protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology, Division of Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The eyes shut protein antigen was originally identified as "Retinal Space (mechanoreceptors?)".
Antigen Molecular Weight: 234.1 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: EYS
Immunogen: Retinal homogenates
Alternate Gene Names: Spacemaker, spam, mAb 21A6
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: The epitope lies between the 5th and 7th EGF repeat at the N-terminus.
Uniprot ID: A0A1F4 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3771890 
Additional Characterization:  
Antibody Registry ID: AB_528449 
Additional Information: The immunization was 5 injections containing material from 20-50 flies through 4 intraperitoneal routes and 1 intravenous injection. The origin of the immunogen is C-S strain. For immunohistochemistry, cryostat sections fixed in 2% formalin for 30 minutes is recommended. RRID:AB_528449. FBgn0031414
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
21A6 was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product 21A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Monoclonal antibodies against the Drosophila nervous system.
    Shotwell SL
    Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

    IF References
    IHC References

    A Change in ECM Composition Affects Sensory Organ Mechanics and Function.
    Tzlil S
    Cell reports 27.8 (2019 May 21): 2272-2280.e4.

    A mosaic of independent innovations involving eyes shut are critical for the evolutionary transition from fused to open rhabdoms.
    Zelhof AC
    Developmental biology 443.2 (2018 Nov 15): 188-202.

    In vivo assembly and trafficking of olfactory Ionotropic Receptors.
    Benton R
    BMC biology 17.1 (2019 Apr 17): 34.

    Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons.
    Su CY
    Nature communications 10.1 (2019 Apr 5): 1560.

    Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis.
    Raghu P
    Journal of cell science 130.13 (2017 Jul 1): 2119-2133.

    Insect odorant receptor trafficking requires calmodulin.
    Jones WD
    BMC biology 14.1 (2016 Sep 29): 83.

    Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.
    Megraw TL
    The Journal of cell biology 211.2 (2015 Oct 26): 435-53.

    Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability.
    Gebelein B
    PloS one 10.8 (2015): e0134915.

    A genetic toolkit for tagging intronic MiMIC containing genes.
    Bellen HJ
    eLife 4. (2015 Jun 23): .

    A quick method to investigate the <i>Drosophila</i> Johnston's organ by confocal microscopy.
    Mishra M
    Journal of microscopy and ultrastructure 3.1 (2015 Jan-Mar): 1-7.

    The protein O-glucosyltransferase Rumi modifies eyes shut to promote rhabdomere separation in Drosophila.
    Jafar-Nejad H
    PLoS genetics 10.11 (2014 Nov): e1004795.

    Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules.
    Basto R
    Biology open 3.2 (2014 Feb 15): 138-51.

    Xbp1-independent Ire1 signaling is required for photoreceptor differentiation and rhabdomere morphogenesis in Drosophila.
    Domingos PM
    Cell reports 5.3 (2013 Nov 14): 791-801.

    dTULP, the Drosophila melanogaster homolog of tubby, regulates transient receptor potential channel localization in cilia.
    Moon SJ
    PLoS genetics 9.9 (2013): e1003814.

    Ionotropic glutamate receptors IR64a and IR8a form a functional odorant receptor complex in vivo in Drosophila.
    Suh GS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.26 (2013 Jun 26): 10741-9.

    Cbl-associated protein regulates assembly and function of two tension-sensing structures in Drosophila.
    Kolodkin AL
    Development (Cambridge, England) 140.3 (2013 Feb 1): 627-38.

    Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation.
    Eberl DF
    Proceedings of the National Academy of Sciences of the United States of America 110.1 (2013 Jan 2): 181-6.

    Increased levels of the cytoplasmic domain of Crumbs repolarise developing Drosophila photoreceptors.
    Knust E
    Journal of cell science 124.Pt 21 (2011 Nov 1): 3715-25.

    Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila.
    Vosshall LB
    Cell 136.1 (2009 Jan 9): 149-62.

    IP References
    Epitope Map References

    Transforming the architecture of compound eyes.
    Zuker CS
    Nature 443.7112 (2006 Oct 12): 696-9.

    All References

    A Change in ECM Composition Affects Sensory Organ Mechanics and Function.
    Tzlil S
    Cell reports 27.8 (2019 May 21): 2272-2280.e4.

    A mosaic of independent innovations involving eyes shut are critical for the evolutionary transition from fused to open rhabdoms.
    Zelhof AC
    Developmental biology 443.2 (2018 Nov 15): 188-202.

    In vivo assembly and trafficking of olfactory Ionotropic Receptors.
    Benton R
    BMC biology 17.1 (2019 Apr 17): 34.

    Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons.
    Su CY
    Nature communications 10.1 (2019 Apr 5): 1560.

    Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis.
    Raghu P
    Journal of cell science 130.13 (2017 Jul 1): 2119-2133.

    Insect odorant receptor trafficking requires calmodulin.
    Jones WD
    BMC biology 14.1 (2016 Sep 29): 83.

    Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.
    Megraw TL
    The Journal of cell biology 211.2 (2015 Oct 26): 435-53.

    Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability.
    Gebelein B
    PloS one 10.8 (2015): e0134915.

    A genetic toolkit for tagging intronic MiMIC containing genes.
    Bellen HJ
    eLife 4. (2015 Jun 23): .

    A quick method to investigate the <i>Drosophila</i> Johnston's organ by confocal microscopy.
    Mishra M
    Journal of microscopy and ultrastructure 3.1 (2015 Jan-Mar): 1-7.

    The protein O-glucosyltransferase Rumi modifies eyes shut to promote rhabdomere separation in Drosophila.
    Jafar-Nejad H
    PLoS genetics 10.11 (2014 Nov): e1004795.

    Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules.
    Basto R
    Biology open 3.2 (2014 Feb 15): 138-51.

    Xbp1-independent Ire1 signaling is required for photoreceptor differentiation and rhabdomere morphogenesis in Drosophila.
    Domingos PM
    Cell reports 5.3 (2013 Nov 14): 791-801.

    dTULP, the Drosophila melanogaster homolog of tubby, regulates transient receptor potential channel localization in cilia.
    Moon SJ
    PLoS genetics 9.9 (2013): e1003814.

    Ionotropic glutamate receptors IR64a and IR8a form a functional odorant receptor complex in vivo in Drosophila.
    Suh GS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.26 (2013 Jun 26): 10741-9.

    Cbl-associated protein regulates assembly and function of two tension-sensing structures in Drosophila.
    Kolodkin AL
    Development (Cambridge, England) 140.3 (2013 Feb 1): 627-38.

    Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation.
    Eberl DF
    Proceedings of the National Academy of Sciences of the United States of America 110.1 (2013 Jan 2): 181-6.

    Increased levels of the cytoplasmic domain of Crumbs repolarise developing Drosophila photoreceptors.
    Knust E
    Journal of cell science 124.Pt 21 (2011 Nov 1): 3715-25.

    Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila.
    Vosshall LB
    Cell 136.1 (2009 Jan 9): 149-62.

    Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation.
    Zelhof AC
    BMC developmental biology 15. (2015 Feb 3): 10.

    The actomyosin machinery is required for Drosophila retinal lumen formation.
    Zelhof AC
    PLoS genetics 10.9 (2014 Sep): e1004608.

    Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
    Chung YD
    PloS one 5.6 (2010 Jun 8): e11012.

    An IFT-A protein is required to delimit functionally distinct zones in mechanosensory cilia.
    Kernan MJ
    Current biology : CB 18.24 (2008 Dec 23): 1899-906.

    The agrin/perlecan-related protein eyes shut is essential for epithelial lumen formation in the Drosophila retina.
    Tepass U
    Developmental cell 11.4 (2006 Oct): 483-93.

    Monoclonal antibodies against the Drosophila nervous system.
    Shotwell SL
    Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

    Transforming the architecture of compound eyes.
    Zuker CS
    Nature 443.7112 (2006 Oct 12): 696-9.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review