mAb 4D4(A2B5-like)

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SKU: mAb 4D4(A2B5-like)
View product citations for antibody mAb 4D4(A2B5-like) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Glial precursor marker
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Kaprielian, Z.
Isotype: MIgM
Depositors Institution: Albert Einstein College of Medicine
Host Species: mouse
Depositors Notes: The mAb 4D4(A2B5-like) antigen is expressed on glial-restricted precursors. This antibody probably recognizes the same or a closely related epitope recognized by mAb A2B5.
Positive Tested Species Reactivity: Chick, Mouse, Rat
Clonality: Monoclonal
Immunogen: Whole cell suspension derived from intermediate region of embryonic spinal cord
Epitope Mapped: No
Myeloma Strain: NS-1
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_1157905 
Additional Information: RRID: AB_1157905
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 4D4(A2B5-like) was deposited to the DSHB by Kaprielian, Z. (DSHB Hybridoma Product mAb 4D4(A2B5-like))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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