mAb 4D4(A2B5-like)

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SKU: mAb 4D4(A2B5-like)
View product citations for antibody mAb 4D4(A2B5-like) on CiteAb

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Available: 179

DSHB Data Sheet

Catalog Fields

Antigen: Glial precursor marker
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Kaprielian, Z.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Albert Einstein College of Medicine
Positive Tested Species Reactivity: Chick, Mouse, Rat
Depositors Notes: The mAb 4D4(A2B5-like) antigen is expressed on glial-restricted precursors. This antibody probably recognizes the same or a closely related epitope recognized by mAb A2B5.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Whole cell suspension derived from intermediate region of embryonic spinal cord
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length sequence
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_1157905 
Additional Information: RRID: AB_1157905
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 4D4(A2B5-like) was deposited to the DSHB by Kaprielian, Z. (DSHB Hybridoma Product mAb 4D4(A2B5-like))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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