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SKU: 5B5-2
View product citations for antibody 5B5-2 on CiteAb

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Available: 172

DSHB Data Sheet

Catalog Fields

Antigen: Hoxc9
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Mouse
Depositors Notes:
Antigen Molecular Weight: Predicted: 29 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: HOXC9
Immunogen: Recombinant N-terminus region
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: N-terminus region
Uniprot ID: A0A1D5PQW8 
Immunogen Sequence: Partial protein
Entrez Gene ID: 425723 
Additional Characterization:
Antibody Registry ID: AB_528287 
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5B5-2 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 5B5-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • Epitope Map References
  • All References
  • Initial Publication

    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    Epitope Map References

    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    All References

    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

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