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SKU: 45E10
View product citations for antibody 45E10 on CiteAb

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Available: 28

DSHB Data Sheet

Catalog Fields

Antigen: Tyrosine-protein phosphatase 10D
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Depositors Institution: California Institute of Technology
Host Species: mouse
Gene: Ptp10D
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: Dmel_CG1817
Antigen Molecular Weight: 116 kDa
Clonality: Monoclonal
Immunogen: Recombinant protein domain
Epitope Mapped: No
Myeloma Strain: HL-1
Immunogen Sequence: Extracellular domain
Uniprot ID: P35992 
Additional Information: RRID:AB_528442. FBgn0004370.
Entrez Gene ID: 32115 
Antibody Registry ID: AB_528442 
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
45E10 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 45E10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication
    WB References
    IHC References
    All References

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