5G2 anti-enabled

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SKU: 5G2 anti-enabled

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 5G2 anti-enabled
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Ena/enabled
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528220 
Uniprot ID: Q8T4F7 
RRID:  
Entrez Gene ID: 37201 
Clonality: Monoclonal
Immunogen: Enabled (a.a. 105-370) fusion protein tagged with 6X-HIS.
Clone:
Immunogen Sequence: aa105-370
Myeloma Strain: P3 x 63 Ag8.653
Epitope Mapped: No
Antigen Name: Enabled
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/14/2002
Antigen Molecular Weight: 30 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5G2 anti-enabled was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 5G2 anti-enabled)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.
    Fulga TA
    Nature communications 5. (2014 Aug 19): 4640.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing.
    Milán M
    Development (Cambridge, England) 138.17 (2011 Sep): 3781-9.

    A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology.
    Kaufmann N
    Biology open 10.2 (2021 Feb 3): .

    Enabled/VASP is required to mediate proper sealing of opposing cardioblasts during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 250.8 (2021 Aug): 1173-1190.

    WB References

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells.
    Brown NH
    Developmental cell 26.6 (2013 Sep 30): 604-15.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    IHC References
    IP References
    All References

    Developmental axon regrowth and primary neuron sprouting utilize distinct actin elongation factors.
    Schuldiner O
    The Journal of cell biology 219.5 (2020 May 4): .

    Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.
    Fulga TA
    Nature communications 5. (2014 Aug 19): 4640.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing.
    Milán M
    Development (Cambridge, England) 138.17 (2011 Sep): 3781-9.

    A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology.
    Kaufmann N
    Biology open 10.2 (2021 Feb 3): .

    Enabled/VASP is required to mediate proper sealing of opposing cardioblasts during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 250.8 (2021 Aug): 1173-1190.

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells.
    Brown NH
    Developmental cell 26.6 (2013 Sep 30): 604-15.

    Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor.
    Goodman CS
    Cell 101.7 (2000 Jun 23): 703-15.

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