5G2 anti-enabled

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$40.00
SKU: 5G2 anti-enabled
View product citations for antibody 5G2 anti-enabled on CiteAb

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Available: 131

DSHB Data Sheet

Catalog Fields

Antigen: Enabled
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 30 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ena/enabled
Immunogen: Enabled (a.a. 105-370) fusion protein tagged with 6X-HIS.
Alternate Gene Names: Ena/ ENA/VASP/ enb/Enb/ l(2)02029/Vasp/VASP
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q8T4F7 
Immunogen Sequence: aa105-370
Entrez Gene ID: 37201 
Additional Characterization:  
Antibody Registry ID: AB_528220 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5G2 anti-enabled was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 5G2 anti-enabled)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.
    Fulga TA
    Nature communications 5. (2014 Aug 19): 4640.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing.
    Milán M
    Development (Cambridge, England) 138.17 (2011 Sep): 3781-9.

    WB References

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells.
    Brown NH
    Developmental cell 26.6 (2013 Sep 30): 604-15.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    IHC References
    IP References
    All References

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