AFFN-KI67-3E6

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$40.00
SKU: AFFN-KI67-3E6-s
View product citations for antibody AFFN-KI67-3E6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: KI-67; Marker of proliferation Ki-67
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a nuclear protein that may be required for cell proliferation.
Antigen Molecular Weight: 358.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MKI67
Immunogen: Recombinant peptide
Alternate Gene Names: MIB-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: P46013 
Immunogen Sequence: a.a. 1-102
Entrez Gene ID: 4288 
Antibody Registry ID: AB_2617702 
Additional Information: RRID:AB_2617702
Recommended Applications: ELISA, Immunofluorescence, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-KI67-3E6 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-KI67-3E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References
    All References

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