BP 102 anti-CNS axons

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SKU: BP 102 anti-CNS axons

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DSHB Data Sheet

Catalog Fields

Product Name/ID: BP 102 anti-CNS axons
Available to For-Profits: Yes
Alternate Antibody Name: BP102
Gene Name:
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_528099 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: axons, CNS (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight:
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This antibody strongly stains the axons of the CNS with virtually undetectable staining of neuron cell bodies, the PNS, or any other tissues of the embryo. It is an excellent marker for the pattern of commissures and connectives. The antibody can be used to stain dissections without detergent. The antibody probably recognizes a carbohydrate epitope based on its insensitivity to glutaraldehyde and sensitivity to periodate.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 102 anti-CNS axons was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 102 anti-CNS axons)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

    Genetic analysis of a Drosophila neural cell adhesion molecule: interaction of fasciclin I and Abelson tyrosine kinase mutations.
    Goodman CS
    Cell 60.4 (1990 Feb 23): 565-75.

    Programmed cell death in the Drosophila central nervous system midline.
    Nambu JR
    Current biology : CB 5.7 (1995 Jul 1): 784-90.

    The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells.
    Klämbt C
    Development (Cambridge, England) 117.1 (1993 Jan): 163-76.

    Perturbed glial scaffold formation precedes axon tract malformation in Drosophila mutants.
    Jacobs JR
    Journal of neurobiology 24.5 (1993 May): 611-26.

    Approaches to identify genes involved in Drosophila embryonic CNS development.
    Perrimon N
    Journal of neurobiology 24.6 (1993 Jun): 701-22.

    Commissure formation in the embryonic insect brain.
    Hirth F
    Arthropod structure & development 32.1 (2003 Aug): 61-77.

    DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis.
    Bender M
    Proceedings of the National Academy of Sciences of the United States of America 94.22 (1997 Oct 28): 12024-9.

    Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline.
    Goodman CS
    Neuron 10.3 (1993 Mar): 409-26.

    Long disordered regions of the C-terminal domain of Abelson tyrosine kinase have specific and additive functions in regulation and axon localization.
    VanBerkum MFA
    PloS one 12.12 (2017): e0189338.

    Centrosome localization determines neuronal polarity.
    Dotti CG
    Nature 436.7051 (2005 Aug 4): 704-8.

    Drosophila neuromuscular synapse assembly and function require the TGF-beta type I receptor saxophone and the transcription factor Mad.
    Selleck SB
    Journal of neurobiology 55.2 (2003 May): 134-50.

    Identification of common excitatory motoneurons in Drosophila melanogaster larvae.
    Tsujimura H
    Zoological science 24.5 (2007 May): 504-13.

    Wingless activity in the precursor cells specifies neuronal migratory behavior in the Drosophila nerve cord.
    Bhat KM
    Developmental biology 311.2 (2007 Nov 15): 613-22.

    Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection.
    Leulier F
    Journal of innate immunity 1.4 (2009): 322-34.

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