BP 102 anti-CNS axons
Catalog Fields
Clone ID/Product Name: BP 102 anti-CNS axons
Available to For-Profits: Yes
Alternate Antibody Name: BP102
Gene Symbol:
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_528099
Clonality: Monoclonal
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: axons, CNS (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight:
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This antibody strongly stains the axons of the CNS with virtually undetectable staining of neuron cell bodies, the PNS, or any other tissues of the embryo. It is an excellent marker for the pattern of commissures and connectives. The antibody can be used to stain dissections without detergent. The antibody probably recognizes a carbohydrate epitope based on its insensitivity to glutaraldehyde and sensitivity to periodate.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 102 anti-CNS axons was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 102 anti-CNS axons)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
Genetic analysis of a Drosophila neural cell adhesion molecule: interaction of fasciclin I and Abelson tyrosine kinase mutations.
Goodman CS
Cell 60.4 (1990 Feb 23): 565-75.