BP 102 anti-CNS axons

(0) No Reviews yet
$40.00
SKU: BP 102 anti-CNS axons
View product citations for antibody BP 102 anti-CNS axons on CiteAb

In Stock

Available: 145

DSHB Data Sheet

Catalog Fields

Antigen: axons, CNS (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody strongly stains the axons of the CNS with virtually undetectable staining of neuron cell bodies, the PNS, or any other tissues of the embryo. It is an excellent marker for the pattern of commissures and connectives. The antibody can be used to stain dissections without detergent. The antibody probably recognizes a carbohydrate epitope based on its insensitivity to glutaraldehyde and sensitivity to periodate.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Alternate Gene Names:
Alternate Antibody Name: BP102
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528099 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 102 anti-CNS axons was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 102 anti-CNS axons)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

    Genetic analysis of a Drosophila neural cell adhesion molecule: interaction of fasciclin I and Abelson tyrosine kinase mutations.
    Goodman CS
    Cell 60.4 (1990 Feb 23): 565-75.

    Programmed cell death in the Drosophila central nervous system midline.
    Nambu JR
    Current biology : CB 5.7 (1995 Jul 1): 784-90.

    The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells.
    Klämbt C
    Development (Cambridge, England) 117.1 (1993 Jan): 163-76.

    Perturbed glial scaffold formation precedes axon tract malformation in Drosophila mutants.
    Jacobs JR
    Journal of neurobiology 24.5 (1993 May): 611-26.

    Approaches to identify genes involved in Drosophila embryonic CNS development.
    Perrimon N
    Journal of neurobiology 24.6 (1993 Jun): 701-22.

    Commissure formation in the embryonic insect brain.
    Hirth F
    Arthropod structure and development 32.1 (2003 Aug): 61-77.

    DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis.
    Bender M
    Proceedings of the National Academy of Sciences of the United States of America 94.22 (1997 Oct 28): 12024-9.

    Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline.
    Goodman CS
    Neuron 10.3 (1993 Mar): 409-26.

    Long disordered regions of the C-terminal domain of Abelson tyrosine kinase have specific and additive functions in regulation and axon localization.
    VanBerkum MFA
    PloS one 12.12 (2017): e0189338.

    Centrosome localization determines neuronal polarity.
    Dotti CG
    Nature 436.7051 (2005 Aug 4): 704-8.

    Drosophila neuromuscular synapse assembly and function require the TGF-beta type I receptor saxophone and the transcription factor Mad.
    Selleck SB
    Journal of neurobiology 55.2 (2003 May): 134-50.

    Identification of common excitatory motoneurons in Drosophila melanogaster larvae.
    Tsujimura H
    Zoological science 24.5 (2007 May): 504-13.

    Wingless activity in the precursor cells specifies neuronal migratory behavior in the Drosophila nerve cord.
    Bhat KM
    Developmental biology 311.2 (2007 Nov 15): 613-22.

    Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection.
    Leulier F
    Journal of innate immunity 1.4 (2009): 322-34.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review