Antibody Name: BP 104 anti-Neuroglian

Antigen:neuroglian (Drosophila, neuron-specific)

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SKU: BP 104 anti-Neuroglian

Product Form :

Supernatant 1.0 ml

~ 14 – 18 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Catalog Fields

Identity

Clone ID / Antibody Name: BP 104 anti-Neuroglian

Alternate Antibody Name: —

Antigen Name: neuroglian (Drosophila, neuron-specific)

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: Nrg

Entrez Gene ID: 31792

Antigen Sequence: —

Antigen Species: Drosophila

Antigen Mol. Weight: Predicted 144 kDa; Apparent: 180 kDa

Uniprot ID: P20241

Human Protein Atlas: —

Antibody Properties

Host Species: Mouse

Clonality: Monoclonal

Isotype: Mouse IgG1

Myeloma Strain: NS-1

Antibody Registry / RRID: AB_528402

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: Membrane protein preparation from cultured embryonic Drosophila neurons.

Immunogen Sequence: Total protein

Epitope Mapped: Yes

Epitope Location / Seq: Cytoplasmic domain; aa 1236-1302 of isoform B of Uniprot ID P20241

Clone: —

Additional Info: BP 104 requires detergent permeabilization of tissue to bind to the protein, suggesting that it recognizes an epitope specific to the cytoplasmic domain of this form (isoform B) of the protein [PMID 1693086].

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: Drosophila

Predicted Reactivity: —

Depositor

Depositor Name: C. Goodman

Institution: University of California, Berkeley

Deposit Date: February 1, 1994

Depositor Notes: Differential splicing generates a nervous system-specific form of neuroglian. The antibody recognizes a specific form of neuroglian via a cytoplasmic domain generated by alternative splicing. Staining is first seen at about 6 hrs in a subset of neurons, but rapidly expands to include all neurons of the CNS and PNS. It is an exceptionally useful marker for the neurons of the neuron pattern in the PNS and developing eye disc. The PNS staining includes a small number of non-neuronal support cells such as the innermost sheath cell associated with the chordotonal neurons. This antibody works well on Westerns.

Recommended Applications

IHC

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtP20241 ↗

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Could not load UniProt data for P20241

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Antibody Name: BP 104 anti-Neuroglian

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