BP 104 anti-Neuroglian

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$40.00
SKU: BP 104 anti-Neuroglian
View product citations for antibody BP 104 anti-Neuroglian on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuroglian (Drosophila, neuron-specific)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Differential splicing generates a nervous system-specific form of neuroglian. The antibody recognizes a specific form of neuroglian via a cytoplasmic domain generated by alternative splicing. Staining is first seen at about 6 hrs in a subset of neurons, but rapidly expands to include all neurons of the CNS and PNS. It is an exceptionally useful marker for the neurons of the neuron pattern in the PNS and developing eye disc. The PNS staining includes a small number of non-neuronal support cells such as the innermost sheath cell associated with the chordotonal neurons. This antibody works well on Westerns.
Antigen Molecular Weight: Predicted 144 kDa; Apparent: 180 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Nrg
Immunogen: Membrane protein preparation from cultured embryonic Drosophila neurons.
Alternate Gene Names: Nrg180; CG1634
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Cytoplasmic domain; aa 1236-1302 of isoform B of Uniprot ID P20241
Uniprot ID: P20241 
Immunogen Sequence: Total protein
Entrez Gene ID: 31792 
Additional Characterization:
Antibody Registry ID: AB_528402 
Additional Information: BP 104 requires detergent permeabilization of tissue to bind to the protein, suggesting that it recognizes an epitope specific to the cytoplasmic domain of this form (isoform B) of the protein [PMID 1693086].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 104 anti-Neuroglian was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 104 anti-Neuroglian)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.
    Baumann O
    Experimental cell research 270.2 (2001 Nov 1): 176-87.

    The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye.
    Yu SY
    Cell 104.5 (2001 Mar 9): 699-708.

    MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development.
    Van Vactor D
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences 369.1652 (2014 Sep 26): .

    ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform.
    White K
    Current biology : CB 6.12 (1996 Dec 1): 1634-41.

    Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
    Truman JW
    The Journal of comparative neurology 524.13 (2016 Sep 1): 2677-95.

    Refinement of tools for targeted gene expression in Drosophila.
    Rubin GM
    Genetics 186.2 (2010 Oct): 735-55.

    Control of axonal sprouting and dendrite branching by the Nrg-Ank complex at the neuron-glia interface.
    Uemura T
    Current biology : CB 16.16 (2006 Aug 22): 1678-83.

    X11/Mint genes control polarized localization of axonal membrane proteins in vivo.
    Guo M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.19 (2013 May 8): 8575-86.

    Subcellular trafficking of FGF controls tracheal invasion of Drosophila flight muscle.
    Krasnow MA
    Cell 160.1-2 (2015 Jan 15): 313-23.

    Specification of cell fate in the developing eye of Drosophila.
    Basler K
    Development (Cambridge, England). Supplement 1. (1991): 123-30.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
    Truman JW
    The Journal of comparative neurology 524.13 (2016 Sep 1): 2677-95.

    Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons.
    Rumpf S
    Developmental biology 451.1 (2019 Jul 1): 68-78.

    Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling.
    Chien CT
    Current biology : CB 29.9 (2019 May 6): 1445-1459.e3.

    WB References
    IHC References
    IP References
    Epitope Map References
    All References

    Differential splicing generates a nervous system-specific form of Drosophila neuroglian.
    Goodman CS
    Neuron 4.5 (1990 May): 697-709.

    The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization.
    Hortsch M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.12 (2000 Jun 15): 4515-23.

    Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
    Truman JW
    The Journal of comparative neurology 524.13 (2016 Sep 1): 2677-95.

    Perturbed glial scaffold formation precedes axon tract malformation in Drosophila mutants.
    Jacobs JR
    Journal of neurobiology 24.5 (1993 May): 611-26.

    Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.
    Baumann O
    Experimental cell research 270.2 (2001 Nov 1): 176-87.

    The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye.
    Yu SY
    Cell 104.5 (2001 Mar 9): 699-708.

    MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development.
    Van Vactor D
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences 369.1652 (2014 Sep 26): .

    ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform.
    White K
    Current biology : CB 6.12 (1996 Dec 1): 1634-41.

    Refinement of tools for targeted gene expression in Drosophila.
    Rubin GM
    Genetics 186.2 (2010 Oct): 735-55.

    Control of axonal sprouting and dendrite branching by the Nrg-Ank complex at the neuron-glia interface.
    Uemura T
    Current biology : CB 16.16 (2006 Aug 22): 1678-83.

    X11/Mint genes control polarized localization of axonal membrane proteins in vivo.
    Guo M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.19 (2013 May 8): 8575-86.

    Subcellular trafficking of FGF controls tracheal invasion of Drosophila flight muscle.
    Krasnow MA
    Cell 160.1-2 (2015 Jan 15): 313-23.

    Specification of cell fate in the developing eye of Drosophila.
    Basler K
    Development (Cambridge, England). Supplement 1. (1991): 123-30.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons.
    Rumpf S
    Developmental biology 451.1 (2019 Jul 1): 68-78.

    Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling.
    Chien CT
    Current biology : CB 29.9 (2019 May 6): 1445-1459.e3.

    The cytoplasmic domain of the Drosophila cell adhesion molecule neuroglian is not essential for its homophilic adhesive properties in S2 cells.
    Bieber AJ
    The Journal of biological chemistry 270.32 (1995 Aug 11): 18809-17.

    Neuroglian activates Echinoid to antagonize the Drosophila EGF receptor signaling pathway.
    Hsu JC
    Development (Cambridge, England) 130.10 (2003 May): 2051-9.

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