BP 104 anti-Neuroglian
Catalog Fields
Clone ID/Product Name: BP 104 anti-Neuroglian
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Nrg
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 31792
Clonality: Monoclonal
Immunogen: Membrane protein preparation from cultured embryonic Drosophila neurons.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: neuroglian (Drosophila, neuron-specific)
Epitope Location or Sequence: Cytoplasmic domain; aa 1236-1302 of isoform B of Uniprot ID P20241
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Predicted 144 kDa; Apparent: 180 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Differential splicing generates a nervous system-specific form of neuroglian. The antibody recognizes a specific form of neuroglian via a cytoplasmic domain generated by alternative splicing. Staining is first seen at about 6 hrs in a subset of neurons, but rapidly expands to include all neurons of the CNS and PNS. It is an exceptionally useful marker for the neurons of the neuron pattern in the PNS and developing eye disc. The PNS staining includes a small number of non-neuronal support cells such as the innermost sheath cell associated with the chordotonal neurons. This antibody works well on Westerns.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: BP 104 requires detergent permeabilization of tissue to bind to the protein, suggesting that it recognizes an epitope specific to the cytoplasmic domain of this form (isoform B) of the protein [PMID 1693086].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot