C458.2H

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$40.00
SKU: C458.2H
View product citations for antibody C458.2H on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Notch, extracellular domain, EGF repeats #12-20
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody was generated against the extracellular domain of Notch containing the EGF repeats #12-20
Antigen Molecular Weight: Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: N
Immunogen: Recombinant fusion protein containing the EGF repeats #12-20
Alternate Gene Names: CG3936, Notch, NECD, NICD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8
Epitope Location or Sequence: extracellular domain, EGF repeats #12-20 [see depositor notes]
Uniprot ID: P07207 
Immunogen Sequence: Partial protein
Entrez Gene ID: 31293 
Additional Characterization:
Antibody Registry ID: AB_528408 
Additional Information: The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C458.2H was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C458.2H)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 473-81.

    IF References

    Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 473-81.

    UDP-sugar transporter implicated in glycosylation and processing of Notch.
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Regulation of notch signaling by o-linked fucose.
    Irvine KD
    Cell 111.6 (2002 Dec 13): 893-904.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    FKBP14 is an essential gene that regulates Presenilin protein levels and Notch signaling in Drosophila.
    Boulianne GL
    Development (Cambridge, England) 140.4 (2013 Feb): 810-9.

    Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling.
    Irvine KD
    BMC biology 6. (2008 Jan 14): 1.

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    Control of cell proliferation in the Drosophila eye by Notch signaling.
    Freeman M
    Developmental cell 8.4 (2005 Apr): 529-39.

    Mechanisms of ligand-mediated inhibition in Notch signaling activity in Drosophila.
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    Distinct levels of Notch activity for commitment and terminal differentiation of stem cells in the adult fly intestine.
    Bardin AJ
    Development (Cambridge, England) 138.21 (2011 Nov): 4585-95.

    Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster.
    Fortini ME
    The Journal of cell biology 146.6 (1999 Sep 20): 1351-64.

    Notch signaling controls germline stem cell niche formation in the Drosophila ovary.
    Xie T
    Development (Cambridge, England) 134.6 (2007 Mar): 1071-80.

    WB References

    O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors.
    Okajima T
    The Journal of biological chemistry 283.51 (2008 Dec 19): 35486-95.

    Mechanisms of ligand-mediated inhibition in Notch signaling activity in Drosophila.
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    IHC References

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    Fringe-dependent separation of dorsal and ventral cells in the Drosophila wing.
    Irvine KD
    Nature 401.6752 (1999 Sep 30): 476-80.

    IP References

    O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors.
    Okajima T
    The Journal of biological chemistry 283.51 (2008 Dec 19): 35486-95.

    All References

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    Fringe-dependent separation of dorsal and ventral cells in the Drosophila wing.
    Irvine KD
    Nature 401.6752 (1999 Sep 30): 476-80.

    Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 473-81.

    UDP-sugar transporter implicated in glycosylation and processing of Notch.
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Regulation of notch signaling by o-linked fucose.
    Irvine KD
    Cell 111.6 (2002 Dec 13): 893-904.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    FKBP14 is an essential gene that regulates Presenilin protein levels and Notch signaling in Drosophila.
    Boulianne GL
    Development (Cambridge, England) 140.4 (2013 Feb): 810-9.

    Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling.
    Irvine KD
    BMC biology 6. (2008 Jan 14): 1.

    Control of cell proliferation in the Drosophila eye by Notch signaling.
    Freeman M
    Developmental cell 8.4 (2005 Apr): 529-39.

    Mechanisms of ligand-mediated inhibition in Notch signaling activity in Drosophila.
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    Distinct levels of Notch activity for commitment and terminal differentiation of stem cells in the adult fly intestine.
    Bardin AJ
    Development (Cambridge, England) 138.21 (2011 Nov): 4585-95.

    Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster.
    Fortini ME
    The Journal of cell biology 146.6 (1999 Sep 20): 1351-64.

    Notch signaling controls germline stem cell niche formation in the Drosophila ovary.
    Xie T
    Development (Cambridge, England) 134.6 (2007 Mar): 1071-80.

    O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors.
    Okajima T
    The Journal of biological chemistry 283.51 (2008 Dec 19): 35486-95.

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