C458.2H
Catalog Fields
Clone ID/Product Name: C458.2H
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: N
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 31293
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing the EGF repeats #12-20
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: Ag8
Epitope Mapped: Yes
Antigen Name: Notch, extracellular domain, EGF repeats #12-20
Epitope Location or Sequence: extracellular domain, EGF repeats #12-20 [see depositor notes]
Alternate Antigen Name:
Deposit Date: 5/2/2000
Antigen Molecular Weight: Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.
Depositor: Artavanis-Tsakonas, S.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody was generated against the extracellular domain of Notch containing the EGF repeats #12-20
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C458.2H was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C458.2H)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
Artavanis-Tsakonas S
Development (Cambridge, England) 120.3 (1994 Mar): 473-81.