C458.2H
Catalog Fields
Antigen: Notch, extracellular domain, EGF repeats #12-20
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Artavanis-Tsakonas, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Harvard Medical School
Antigen Molecular Weight: Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.
Depositors Notes: This antibody was generated against the extracellular domain of Notch containing the EGF repeats #12-20
Immunogen: Recombinant fusion protein containing the EGF repeats #12-20
Gene: N
Alternate Antibody Name:
Alternate Gene Names: CG3936, Notch, NECD, NICD
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Ag8
Epitope Mapped: Yes
Uniprot ID: P07207
Epitope Location or Sequence: extracellular domain, EGF repeats #12-20 [see depositor notes]
Entrez Gene ID: 31293
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528408
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C458.2H was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C458.2H)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
Artavanis-Tsakonas S
Development (Cambridge, England) 120.3 (1994 Mar): 473-81.