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$45.00 to $425.00
SKU: F461.3B
View product citations for antibody F461.3B on CiteAb

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Available: 138

DSHB Data Sheet

Catalog Fields

Antigen: Notch, extracellular domain, EGF repeats #5-7
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody was generated against the extracellular domain of Notch containing the EGF repeats # 5-7
Antigen Molecular Weight: Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: N
Immunogen: Recombinant fusion protein containing the EGF repeats #5-7
Alternate Gene Names: CG3936, Notch, NECD, NICD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8
Epitope Location or Sequence: extracellular domain, EGF repeats #5-7 [see depositor notes]
Uniprot ID: P07207 
Immunogen Sequence: Partial protein
Entrez Gene ID: 31293 
Additional Characterization:  
Antibody Registry ID: AB_528409 
Additional Information: The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F461.3B was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product F461.3B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    IF References
    WB References

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    Methylmercury induces activation of Notch signaling.
    Rand MD
    Neurotoxicology 27.6 (2006 Dec): 982-91.

    Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA.
    López-Bayghen E
    ASN neuro 5.5 (2013 Dec 23): e00130.

    All References

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