Costal-2 (17E11)

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$45.00 to $425.00
SKU: Costal-2 (17E11)
View product citations for antibody Costal-2 (17E11) on CiteAb

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Available: 73

DSHB Data Sheet

Catalog Fields

Antigen: Costal-2 (cos)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Beachy, P.A.
Isotype: MIgG2a, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University, Developmental Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't seem to work for western blotting
Antigen Molecular Weight: Predicted: 133 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cos
Immunogen: Recombinant N-terminal motor and neck domains (aa 1-640) fused in-frame with the Fc region of human IgG, expressed in CHO cells.
Alternate Gene Names: cos2, costa, CG1708
Alternate Antibody Name: 17E11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: N-terminal motor and neck domains (aa 1-640)
Uniprot ID: O16844 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35653 
Additional Characterization:  
Antibody Registry ID: AB_528179 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Costal-2 (17E11) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Costal-2 (17E11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    ELISA References
    Epitope Map References
    All References

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