Smoothened (20C6)

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$40.00
SKU: Smoothened (20C6)
View product citations for antibody Smoothened (20C6) on CiteAb

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Available: 177

DSHB Data Sheet

Catalog Fields

Antigen: Smoothened (Smo)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Beachy, P.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Doesn't appear to work for Immunoblotting.
Antigen Molecular Weight: Predicted: 116 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: smo
Immunogen: Recombinant N-terminal first extracellular domain (aa 1-257) fused in-frame with the Fc region of human IgG
Alternate Gene Names: CG11561
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: N-terminal first extracellular domain (aa 1-257)
Uniprot ID: P91682 
Immunogen Sequence: Partial protein
Entrez Gene ID: 33196 
Additional Characterization:  
Antibody Registry ID: AB_528472 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Smoothened (20C6) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Smoothened (20C6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
    Beachy PA
    Molecular cell 12.5 (2003 Nov): 1261-74.

    Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner.
    Jia J
    Developmental biology 366.2 (2012 Jun 15): 172-84.

    Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye.
    Moses K
    Mechanisms of development 123.2 (2006 Feb): 151-65.

    Sequential phosphorylation of smoothened transduces graded hedgehog signaling.
    Zhu AJ
    Science signaling 4.180 (2011 Jul 5): ra43.

    Evidence for a novel feedback loop in the Hedgehog pathway involving Smoothened and Fused.
    Plessis A
    Current biology : CB 17.15 (2007 Aug 7): 1326-33.

    The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway.
    Plessis A
    Developmental biology 303.1 (2007 Mar 1): 121-33.

    Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex.
    Ruel L
    Nature communications 5. (2014 Oct 7): 5034.

    Drosophila miR-960 negatively regulates Hedgehog signaling by suppressing Smoothened, Costal-2 and Fused.
    Lin X
    Cellular signalling 25.5 (2013 May): 1301-9.

    Distinct phosphorylations on kinesin costal-2 mediate differential hedgehog signaling strength.
    Thérond PP
    Developmental cell 22.2 (2012 Feb 14): 279-94.

    Drosophila miR-932 modulates hedgehog signaling by targeting its co-receptor Brother of ihog.
    Lin X
    Developmental biology 377.1 (2013 May 1): 166-76.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.
    Zhou Z
    Biochemical and biophysical research communications 513.4 (2019 Jun 11): 1013-1018.

    IP References

    Extensive phosphorylation of Smoothened in Hedgehog pathway activation.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 101.52 (2004 Dec 28): 17900-7.

    Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex.
    Ruel L
    Nature communications 5. (2014 Oct 7): 5034.

    Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
    Beachy PA
    Molecular cell 12.5 (2003 Nov): 1261-74.

    Epitope Map References
    All References

    Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
    Beachy PA
    Molecular cell 12.5 (2003 Nov): 1261-74.

    Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner.
    Jia J
    Developmental biology 366.2 (2012 Jun 15): 172-84.

    Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye.
    Moses K
    Mechanisms of development 123.2 (2006 Feb): 151-65.

    Sequential phosphorylation of smoothened transduces graded hedgehog signaling.
    Zhu AJ
    Science signaling 4.180 (2011 Jul 5): ra43.

    Evidence for a novel feedback loop in the Hedgehog pathway involving Smoothened and Fused.
    Plessis A
    Current biology : CB 17.15 (2007 Aug 7): 1326-33.

    The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway.
    Plessis A
    Developmental biology 303.1 (2007 Mar 1): 121-33.

    Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex.
    Ruel L
    Nature communications 5. (2014 Oct 7): 5034.

    Drosophila miR-960 negatively regulates Hedgehog signaling by suppressing Smoothened, Costal-2 and Fused.
    Lin X
    Cellular signalling 25.5 (2013 May): 1301-9.

    Distinct phosphorylations on kinesin costal-2 mediate differential hedgehog signaling strength.
    Thérond PP
    Developmental cell 22.2 (2012 Feb 14): 279-94.

    Drosophila miR-932 modulates hedgehog signaling by targeting its co-receptor Brother of ihog.
    Lin X
    Developmental biology 377.1 (2013 May 1): 166-76.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.
    Zhou Z
    Biochemical and biophysical research communications 513.4 (2019 Jun 11): 1013-1018.

    Extensive phosphorylation of Smoothened in Hedgehog pathway activation.
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 101.52 (2004 Dec 28): 17900-7.

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