Smoothened (20C6)
Catalog Fields
Product Name/ID: Smoothened (20C6)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: smo
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 33196
Clonality: Monoclonal
Immunogen: Recombinant N-terminal first extracellular domain (aa 1-257) fused in-frame with the Fc region of human IgG
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Smoothened (Smo)
Epitope Location or Sequence: N-terminal first extracellular domain (aa 1-257)
Alternate Antigen Name:
Deposit Date: 10/21/2003
Antigen Molecular Weight: Predicted: 116 kDa
Depositor: Beachy, P.A.
Antigen Sequence:
Depositor Institution: Stanford University
Antigen Species: Drosophila
Depositor Notes: Doesn't appear to work for Immunoblotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Smoothened (20C6) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Smoothened (20C6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2.
Beachy PA
Molecular cell 12.5 (2003 Nov): 1261-74.