Dmp53 H3

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SKU: Dmp53 H3
View product citations for antibody Dmp53 H3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: p53
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Ollmann, M.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: Exelixis Inc.
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple isoforms of different MW; Apparent 50 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: p53
Immunogen: Recombinant GST fusion protein of Drosophila p53.
Alternate Gene Names: CG33336, prac
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9N6D8 
Immunogen Sequence: Full length protein
Entrez Gene ID: 2768677 
Additional Characterization:
Antibody Registry ID: AB_10804170 
Additional Information: p53 is the homolog of the Cellular tumor antigen p53. The isoform used for immunisation was not specified.
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dmp53 H3 was deposited to the DSHB by Ollmann, M. (DSHB Hybridoma Product Dmp53 H3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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  • Initial Publication
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