p53 7A4

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SKU: p53 7A4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: p53 7A4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: p53
Ab Isotype: MIgG1, lambda light chain
Gene Symbol:
Antibody Registry ID: AB_579786 
Uniprot ID: Q9N6D8 
RRID:  
Entrez Gene ID: 2768677 
Clonality: Monoclonal
Immunogen: Recombinant GST fusion protein of Drosophila p53 (aa 282-386 [Uniprot ID Q9N6D8]; common to all isoforms)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Antigen Name: p53
Epitope Location or Sequence: aa 282-386
Alternate Antigen Name:
Deposit Date: 8/2/2006
Antigen Molecular Weight: Multiple isoforms of different MW; Apparent 50 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This antibody has been succesfully used for Immunoprecipitation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antibody recognizes the C-terminal region common to all Drosophila p53 isoforms. p53 is the homolog of the Cellular tumor antigen p53.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p53 7A4 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product p53 7A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop.
    Golic KG
    PLoS genetics 11.7 (2015 Jul): e1005400.

    Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.
    Igaki T
    Nature communications 5. (2014 Oct 27): 5264.

    WB References

    Evaluating Drosophila p53 as a model system for studying cancer mutations.
    Segal D
    The Journal of biological chemistry 287.53 (2012 Dec 28): 44330-7.

    Epitope Map References
    All References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop.
    Golic KG
    PLoS genetics 11.7 (2015 Jul): e1005400.

    Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.
    Igaki T
    Nature communications 5. (2014 Oct 27): 5264.

    Evaluating Drosophila p53 as a model system for studying cancer mutations.
    Segal D
    The Journal of biological chemistry 287.53 (2012 Dec 28): 44330-7.

    Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage.
    Rubin GM
    Molecular and cellular biology 24.3 (2004 Feb): 1219-31.

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