p53 7A4

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$40.00
SKU: p53 7A4
View product citations for antibody p53 7A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: p53
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody has been succesfully used for Immunoprecipitation.
Antigen Molecular Weight: Multiple isoforms of different MW; Apparent 50 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: p53
Immunogen: Recombinant GST fusion protein of Drosophila p53 (aa 282-386 [Uniprot ID Q9N6D8]; common to all isoforms)
Alternate Gene Names: CG33336, prac
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence: aa 282-386
Uniprot ID: Q9N6D8 
Immunogen Sequence: Partial protein
Entrez Gene ID: 2768677 
Additional Characterization:
Antibody Registry ID: AB_579786 
Additional Information: This antibody recognizes the C-terminal region common to all Drosophila p53 isoforms. p53 is the homolog of the Cellular tumor antigen p53.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p53 7A4 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product p53 7A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop.
    Golic KG
    PLoS genetics 11.7 (2015 Jul): e1005400.

    Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.
    Igaki T
    Nature communications 5. (2014 Oct 27): 5264.

    WB References

    Evaluating Drosophila p53 as a model system for studying cancer mutations.
    Segal D
    The Journal of biological chemistry 287.53 (2012 Dec 28): 44330-7.

    Epitope Map References
    All References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop.
    Golic KG
    PLoS genetics 11.7 (2015 Jul): e1005400.

    Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.
    Igaki T
    Nature communications 5. (2014 Oct 27): 5264.

    Evaluating Drosophila p53 as a model system for studying cancer mutations.
    Segal D
    The Journal of biological chemistry 287.53 (2012 Dec 28): 44330-7.

    Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage.
    Rubin GM
    Molecular and cellular biology 24.3 (2004 Feb): 1219-31.

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