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View product citations for antibody F2F4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cyclin B
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: O’Farrell, P.H.
Isotype: MIgG1
Depositors Institution: Department of Biochemistry, University of California
Host Species: mouse
Depositors Notes: Immunoblotting using supernatant diluted 1/5
Positive Tested Species Reactivity: Drosophila, Planaria
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antibody Registry ID: AB_2245815 
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F2F4 was deposited to the DSHB by O’Farrell, P.H. (DSHB Hybridoma Product F2F4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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