p53 25F4

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$45.00 to $425.00
SKU: p53 25F4
View product citations for antibody p53 25F4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: p53
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a, lambda light chain
Depositor: Rubin, G.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of California, Berkeley
Antigen Molecular Weight: Multiple isoforms of different MW; Apparent 50 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant GST fusion protein of Drosophila p53 (aa 282-386 [Uniprot ID Q9N6D8]; common to all isoforms)
Gene: p53
Alternate Antibody Name:
Alternate Gene Names: CG33336, prac
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Uniprot ID: Q9N6D8 
Epitope Location or Sequence: aa 282-386
Entrez Gene ID: 2768677 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_579787 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: This antibody recognizes the C-terminal region common to all Drosophila p53 isoforms. p53 is the homolog of the Cellular tumor antigen p53.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p53 25F4 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product p53 25F4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    p53 and PI3K/AKT signalings are up-regulated in flies with defects in the THO complex.
    Chung YD
    Molecules and cells 35.3 (2013 Mar): 261-8.

    Drosophila p53 isoforms differentially regulate apoptosis and apoptosis-induced proliferation.
    Mollereau B
    Cell death and differentiation 20.1 (2013 Jan): 108-16.

    A platform for interrogating cancer-associated p53 alleles.
    Abrams JM
    Oncogene 36.2 (2017 Jan 12): 286-291.

    Dmp53 is sequestered to nuclear bodies in spermatogonia of Drosophila melanogaster.
    Hime GR
    Cell and tissue research 350.2 (2012 Nov): 385-94.

    A Robust Transposon-Endogenizing Response from Germline Stem Cells.
    Zhang ZZ
    Developmental cell 47.5 (2018 Dec 3): 660-671.e3.

    WB References
    IP References
    Epitope Map References
    All References

    Drosophila p53 controls Notch expression and balances apoptosis and proliferation.
    Busturia A
    Apoptosis : an international journal on programmed cell death 19.10 (2014 Oct): 1430-43.

    p53 and PI3K/AKT signalings are up-regulated in flies with defects in the THO complex.
    Chung YD
    Molecules and cells 35.3 (2013 Mar): 261-8.

    Drosophila p53 isoforms differentially regulate apoptosis and apoptosis-induced proliferation.
    Mollereau B
    Cell death and differentiation 20.1 (2013 Jan): 108-16.

    A platform for interrogating cancer-associated p53 alleles.
    Abrams JM
    Oncogene 36.2 (2017 Jan 12): 286-291.

    Dmp53 is sequestered to nuclear bodies in spermatogonia of Drosophila melanogaster.
    Hime GR
    Cell and tissue research 350.2 (2012 Nov): 385-94.

    A Robust Transposon-Endogenizing Response from Germline Stem Cells.
    Zhang ZZ
    Developmental cell 47.5 (2018 Dec 3): 660-671.e3.

    Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage.
    Fang M
    PloS one 8.11 (2013): e78652.

    Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage.
    Rubin GM
    Molecular and cellular biology 24.3 (2004 Feb): 1219-31.

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