Draper 5D14

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$40.00
SKU: Draper 5D14
View product citations for antibody Draper 5D14 on CiteAb

In Stock

Available: 108

DSHB Data Sheet

Catalog Fields

Antigen: Draper
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Logan, M.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oregon Health and Science University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Fixation: 4% PFA for whole mount.
Antigen Molecular Weight: Predicted: Multiple isforms between 59 and 114 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: drpr
Immunogen: NPIVYNESLK
Alternate Gene Names: CG2086
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: NPIVYNESLK
Immunogen Sequence: Partial protein
Entrez Gene ID: 38218 
Additional Characterization:
Antibody Registry ID: AB_2618105 
Additional Information: Draper is an engulfment receptor involved in cell death and phagocytosis. This antibody will recognize all Draper isoforms.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Draper 5D14 was deposited to the DSHB by Logan, M. (DSHB Hybridoma Product Draper 5D14)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Delayed glial clearance of degenerating axons in aged Drosophila is due to reduced PI3K/Draper activity.
    Logan MA
    Nature communications 7. (2016 Sep 20): 12871.

    Detection of Cell Death and Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1328. (2015): 191-206.

    Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary.
    McCall K
    Proceedings of the National Academy of Sciences of the United States of America 113.9 (2016 Mar 1): E1246-55.

    Analysis of Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 79-95.

    Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary.
    McCall K
    Disease models and mechanisms 8.12 (2015 Dec): 1603-14.

    Insulin-like Signaling Promotes Glial Phagocytic Clearance of Degenerating Axons through Regulation of Draper.
    Logan MA
    Cell reports 16.7 (2016 Aug 16): 1838-50.

    WB References
    Epitope Map References
    All References

    Delayed glial clearance of degenerating axons in aged Drosophila is due to reduced PI3K/Draper activity.
    Logan MA
    Nature communications 7. (2016 Sep 20): 12871.

    Detection of Cell Death and Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1328. (2015): 191-206.

    Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary.
    McCall K
    Proceedings of the National Academy of Sciences of the United States of America 113.9 (2016 Mar 1): E1246-55.

    Analysis of Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 79-95.

    Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary.
    McCall K
    Disease models and mechanisms 8.12 (2015 Dec): 1603-14.

    Insulin-like Signaling Promotes Glial Phagocytic Clearance of Degenerating Axons through Regulation of Draper.
    Logan MA
    Cell reports 16.7 (2016 Aug 16): 1838-50.

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