Draper 5D14

(0)No Reviews yet
$45.00 to $425.00
SKU: Draper 5D14
View product citations for antibody Draper 5D14 on CiteAb

In Stock

Available: 158

DSHB Data Sheet

Catalog Fields

Product Name/ID: Draper 5D14
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: drpr
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2618105 
RRID:  
Entrez Gene ID: 38218 
Clonality: Monoclonal
Immunogen: NPIVYNESLK
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Draper
Epitope Location or Sequence: NPIVYNESLK
Alternate Antigen Name:
Deposit Date: 12/13/2012
Antigen Molecular Weight: Predicted: Multiple isforms between 59 and 114 kDa
Depositor: Logan, M.
Antigen Sequence:
Depositor Institution: Oregon Health and Science University
Antigen Species: Drosophila melanogaster
Depositor Notes: Fixation: 4% PFA for whole mount.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Draper is an engulfment receptor involved in cell death and phagocytosis. This antibody will recognize all Draper isoforms.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Draper 5D14 was deposited to the DSHB by Logan, M. (DSHB Hybridoma Product Draper 5D14)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Delayed glial clearance of degenerating axons in aged Drosophila is due to reduced PI3K/Draper activity.
    Logan MA
    Nature communications 7. (2016 Sep 20): 12871.

    Detection of Cell Death and Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1328. (2015): 191-206.

    Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary.
    McCall K
    Proceedings of the National Academy of Sciences of the United States of America 113.9 (2016 Mar 1): E1246-55.

    Analysis of Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 79-95.

    Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary.
    McCall K
    Disease models & mechanisms 8.12 (2015 Dec): 1603-14.

    Insulin-like Signaling Promotes Glial Phagocytic Clearance of Degenerating Axons through Regulation of Draper.
    Logan MA
    Cell reports 16.7 (2016 Aug 16): 1838-50.

    WB References
    Epitope Map References
    All References

    Delayed glial clearance of degenerating axons in aged Drosophila is due to reduced PI3K/Draper activity.
    Logan MA
    Nature communications 7. (2016 Sep 20): 12871.

    Detection of Cell Death and Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1328. (2015): 191-206.

    Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary.
    McCall K
    Proceedings of the National Academy of Sciences of the United States of America 113.9 (2016 Mar 1): E1246-55.

    Analysis of Phagocytosis in the Drosophila Ovary.
    McCall K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 79-95.

    Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary.
    McCall K
    Disease models & mechanisms 8.12 (2015 Dec): 1603-14.

    Insulin-like Signaling Promotes Glial Phagocytic Clearance of Degenerating Axons through Regulation of Draper.
    Logan MA
    Cell reports 16.7 (2016 Aug 16): 1838-50.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review