Draper 8A1

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SKU: Draper 8A1
View product citations for antibody Draper 8A1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Draper
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Logan, M.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oregon Health and Science University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Fixation: 4% PFA for whole mount.
Antigen Molecular Weight: Predicted: Multiple isforms between 59 and 114 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: drpr
Alternate Gene Names: CG2086
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: NPIVYNESLK
Immunogen Sequence: Partial protein
Entrez Gene ID: 38218 
Additional Characterization:  
Antibody Registry ID: AB_2618106 
Additional Information: Draper is an engulfment receptor involved in cell death and phagocytosis. This antibody will recognize all Draper isoforms.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Draper 8A1 was deposited to the DSHB by Logan, M. (DSHB Hybridoma Product Draper 8A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
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