Draper 8A1

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SKU: Draper 8A1
View product citations for antibody Draper 8A1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Draper
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Logan, M.
Isotype: MIgG2a
Depositors Institution: Oregon Health and Science University
Host Species: mouse
Depositors Notes: Fixation: 4% PFA for whole mount.
Positive Tested Species Reactivity: Drosophila
Gene: drpr
Antigen Molecular Weight: Predicted: Multiple isforms between 59 and 114 kDa
Alternate Gene Names: CG2086
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Epitope Location or Sequence: NPIVYNESLK
Entrez Gene ID: 38218 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2618106 
Additional Information: Draper is an engulfment receptor involved in cell death and phagocytosis. This antibody will recognize all Draper isoforms.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Draper 8A1 was deposited to the DSHB by Logan, M. (DSHB Hybridoma Product Draper 8A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
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