Draper 8A1

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SKU: Draper 8A1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Draper 8A1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: drpr
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2618106 
Entrez Gene ID: 38218 
Clonality: Monoclonal
Immunogen Sequence: Partial protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Draper
Epitope Location or Sequence: NPIVYNESLK
Alternate Antigen Name:
Deposit Date: 12/13/2012
Antigen Molecular Weight: Predicted: Multiple isforms between 59 and 114 kDa
Depositor: Logan, M.
Antigen Sequence:
Depositor Institution: Oregon Health and Science University
Antigen Species: Drosophila melanogaster
Depositor Notes: Fixation: 4% PFA for whole mount.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Draper is an engulfment receptor involved in cell death and phagocytosis. This antibody will recognize all Draper isoforms.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Draper 8A1 was deposited to the DSHB by Logan, M. (DSHB Hybridoma Product Draper 8A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
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