DsxDBD

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$45.00 to $425.00
SKU: DsxDBD
View product citations for antibody DsxDBD on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Doublesex protein DNA binding domain (DBD)
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Baker, B.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Janelia Farm Research / HHMI
Antigen Molecular Weight: 81.2
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Polypeptide
Gene: Dsx
Alternate Antibody Name:
Alternate Gene Names: CG42234; Dmel_CG42234
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/Il6
Epitope Mapped: Yes
Uniprot ID: P23023 
Epitope Location or Sequence: DNA Binding Domain
Entrez Gene ID: 40940 
Immunogen Sequence:
Antibody Registry ID: AB_2617197 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: RRID:AB_2617197
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DsxDBD was deposited to the DSHB by Baker, B.S. (DSHB Hybridoma Product DsxDBD)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Multiple recent co-options of Optix associated with novel traits in adaptive butterfly wing radiations.
    Reed RD
    EvoDevo 5.1 (2014 Feb 5): 7.

    doublesex is a mimicry supergene.
    Kronforst MR
    Nature 507.7491 (2014 Mar 13): 229-32.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    IHC References
    All References

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    Identification and characterization of sexually dimorphic neurons that express the sex-determining gene doublesex in the brain of silkmoth Bombyx mori.
    Kiya T
    Insect biochemistry and molecular biology 129. (2021 Feb): 103518.

    Novel Doublesex Duplication Associated with Sexually Dimorphic Development of Dogface Butterfly Wings.
    Counterman BA
    Molecular biology and evolution 38.11 (2021 Oct 27): 5021-5033.

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Multiple recent co-options of Optix associated with novel traits in adaptive butterfly wing radiations.
    Reed RD
    EvoDevo 5.1 (2014 Feb 5): 7.

    doublesex is a mimicry supergene.
    Kronforst MR
    Nature 507.7491 (2014 Mar 13): 229-32.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

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