DsxDBD

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$40.00
SKU: DsxDBD
View product citations for antibody DsxDBD on CiteAb

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Available: 137

DSHB Data Sheet

Catalog Fields

Antigen: Doublesex protein DNA binding domain (DBD)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Baker, B.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Janelia Farm Research Campus, HHMI
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 81.2
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Dsx
Immunogen: Polypeptide
Alternate Gene Names: CG42234; Dmel_CG42234
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/Il6
Epitope Location or Sequence: DNA Binding Domain
Uniprot ID: P23023 
Immunogen Sequence:
Entrez Gene ID: 40940 
Additional Characterization:  
Antibody Registry ID: AB_2617197 
Additional Information: RRID:AB_2617197
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DsxDBD was deposited to the DSHB by Baker, B.S. (DSHB Hybridoma Product DsxDBD)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Multiple recent co-options of Optix associated with novel traits in adaptive butterfly wing radiations.
    Reed RD
    EvoDevo 5.1 (2014 Feb 5): 7.

    doublesex is a mimicry supergene.
    Kronforst MR
    Nature 507.7491 (2014 Mar 13): 229-32.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    IHC References
    All References

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Multiple recent co-options of Optix associated with novel traits in adaptive butterfly wing radiations.
    Reed RD
    EvoDevo 5.1 (2014 Feb 5): 7.

    doublesex is a mimicry supergene.
    Kronforst MR
    Nature 507.7491 (2014 Mar 13): 229-32.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

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