anti-ABD-B (1A2E9)

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$40.00
SKU: anti-ABD-B (1A2E9)
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DSHB Data Sheet

Catalog Fields

Antigen: Abdominal-B homeobox protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Celniker, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Genome Dynamics, Lawrence Berkeley National Lab
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Immunohistochemistry: spatial distribution in 1) wild-type embryos [Genes Dev. 3, 1424-1436], 2) Abd-B mutant embryos [EMBO J. 9, 4277-4286], and 3) Transabdominal mutant wing discs [PNAS USA 90, 1566-1570].
Antigen Molecular Weight: Predicted: 56 and 30kDa; Apparent: 54 and 36kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Abd-B
Immunogen: Drosophila Abd-B homeodomain/fusion protein trpE/Abd-B fusion protein semi-purified from whole-cell lysates by acrylamide gel electrophoresis
Alternate Gene Names: bex; BX-C; DmAbdB; Dmel\CG11648; Droabdb; FAB;
Alternate Antibody Name: anti-ABD-B; anti-ABDB; 1A2E9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: a.a. 270-376
Uniprot ID: P09087 
Immunogen Sequence: 270-376 aa region of isoform M
Entrez Gene ID: 47763 
Additional Characterization:  
Antibody Registry ID: AB_528061 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-ABD-B (1A2E9) was deposited to the DSHB by Celniker, S. (DSHB Hybridoma Product anti-ABD-B (1A2E9))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

41 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain.
    Lewis EB
    Genes & development 3.9 (1989 Sep): 1424-36.

    Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain.
    Lee T
    Development (Cambridge, England) 133.7 (2006 Apr): 1231-40.

    Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila.
    Yoder JH
    Proceedings of the National Academy of Sciences of the United States of America 108.27 (2011 Jul 5): 11139-44.

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
    Gould AP
    PLoS biology 6.3 (2008 Mar 11): e58.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Hox proteins mediate developmental and environmental control of autophagy.
    Graba Y
    Developmental cell 28.1 (2014 Jan 13): 56-69.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila.
    Pret AM
    PloS one 5.11 (2010 Nov 11): e13946.

    Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila.
    Yoder JH
    Developmental dynamics : an official publication of the American Association of Anatomists 241.6 (2012 Jun): 1076-90.

    A mutation in the E(Z) methyltransferase that increases trimethylation of histone H3 lysine 27 and causes inappropriate silencing of active Polycomb target genes.
    Harte PJ
    Developmental biology 364.2 (2012 Apr 15): 249-58.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    Homeotic functions of the Teashirt transcription factor during adult Drosophila development.
    Yoder JH
    Biology open 2.1 (2013 Jan 15): 18-29.

    Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites.
    Jan YN
    Genes & development 21.8 (2007 Apr 15): 956-72.

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Homeotic gene action in embryonic brain development of Drosophila.
    Reichert H
    Development (Cambridge, England) 125.9 (1998 May): 1579-89.

    The Hox gene Abdominal-B antagonizes appendage development in the genital disc of Drosophila.
    Sánchez-Herrero E
    Development (Cambridge, England) 128.3 (2001 Feb): 331-9.

    The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.
    Nakagoshi H
    PloS one 7.3 (2012): e32302.

    Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.
    Sémériva M
    Development (Cambridge, England) 129.19 (2002 Oct): 4509-21.

    Polycomb group genes Psc and Su(z)2 maintain somatic stem cell identity and activity in Drosophila.
    Fuller MT
    PloS one 7.12 (2012): e52892.

    Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
    Brahmachari V
    Genes to cells : devoted to molecular & cellular mechanisms 15.7 (2010 Jun): 725-35.

    The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning.
    Renkawitz R
    The EMBO journal 26.19 (2007 Oct 3): 4203-14.

    Comparative analysis of Hox downstream genes in Drosophila.
    Lohmann I
    Development (Cambridge, England) 134.2 (2007 Jan): 381-92.

    Drosophila Hox and sex-determination genes control segment elimination through EGFR and extramacrochetae activity.
    Sánchez-Herrero E
    PLoS genetics 8.8 (2012): e1002874.

    Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function.
    Hombría JC
    PLoS genetics 9.2 (2013): e1003252.

    Polycomb group genes Psc and Su(z)2 restrict follicle stem cell self-renewal and extrusion by controlling canonical and noncanonical Wnt signaling.
    Xi R
    Genes & development 24.9 (2010 May): 933-46.

    Transcriptional regulation of Drosophila gonad formation.
    Renault AD
    Developmental biology 392.2 (2014 Aug 15): 193-208.

    Bithorax-complex genes sculpt the pattern of leucokinergic neurons in the Drosophila central nervous system.
    Díaz-Benjumea FJ
    Development (Cambridge, England) 140.10 (2013 May): 2139-48.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    WB References
    IHC References

    Molecular basis of transabdominal--a sexually dimorphic mutant of the bithorax complex of Drosophila.
    Lewis EB
    Proceedings of the National Academy of Sciences of the United States of America 90.4 (1993 Feb 15): 1566-70.

    The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain.
    Lewis EB
    Genes & development 3.9 (1989 Sep): 1424-36.

    The enhancer of trithorax and polycomb corto interacts with cyclin G in Drosophila.
    Peronnet F
    PloS one 3.2 (2008 Feb 20): e1658.

    Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila.
    Yoder JH
    Developmental dynamics : an official publication of the American Association of Anatomists 241.6 (2012 Jun): 1076-90.

    Mis-specified cells die by an active gene-directed process, and inhibition of this death results in cell fate transformation in Drosophila.
    Bergmann A
    Development (Cambridge, England) 132.24 (2005 Dec): 5343-52.

    Epitope Map References
    Gel Supershift References
    All References

    Molecular basis of transabdominal--a sexually dimorphic mutant of the bithorax complex of Drosophila.
    Lewis EB
    Proceedings of the National Academy of Sciences of the United States of America 90.4 (1993 Feb 15): 1566-70.

    The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain.
    Lewis EB
    Genes & development 3.9 (1989 Sep): 1424-36.

    The enhancer of trithorax and polycomb corto interacts with cyclin G in Drosophila.
    Peronnet F
    PloS one 3.2 (2008 Feb 20): e1658.

    Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila.
    Yoder JH
    Developmental dynamics : an official publication of the American Association of Anatomists 241.6 (2012 Jun): 1076-90.

    Mis-specified cells die by an active gene-directed process, and inhibition of this death results in cell fate transformation in Drosophila.
    Bergmann A
    Development (Cambridge, England) 132.24 (2005 Dec): 5343-52.

    Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain.
    Lee T
    Development (Cambridge, England) 133.7 (2006 Apr): 1231-40.

    Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila.
    Yoder JH
    Proceedings of the National Academy of Sciences of the United States of America 108.27 (2011 Jul 5): 11139-44.

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
    Gould AP
    PLoS biology 6.3 (2008 Mar 11): e58.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Hox proteins mediate developmental and environmental control of autophagy.
    Graba Y
    Developmental cell 28.1 (2014 Jan 13): 56-69.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila.
    Pret AM
    PloS one 5.11 (2010 Nov 11): e13946.

    A mutation in the E(Z) methyltransferase that increases trimethylation of histone H3 lysine 27 and causes inappropriate silencing of active Polycomb target genes.
    Harte PJ
    Developmental biology 364.2 (2012 Apr 15): 249-58.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    Homeotic functions of the Teashirt transcription factor during adult Drosophila development.
    Yoder JH
    Biology open 2.1 (2013 Jan 15): 18-29.

    Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites.
    Jan YN
    Genes & development 21.8 (2007 Apr 15): 956-72.

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Homeotic gene action in embryonic brain development of Drosophila.
    Reichert H
    Development (Cambridge, England) 125.9 (1998 May): 1579-89.

    The Hox gene Abdominal-B antagonizes appendage development in the genital disc of Drosophila.
    Sánchez-Herrero E
    Development (Cambridge, England) 128.3 (2001 Feb): 331-9.

    The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.
    Nakagoshi H
    PloS one 7.3 (2012): e32302.

    Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.
    Sémériva M
    Development (Cambridge, England) 129.19 (2002 Oct): 4509-21.

    Polycomb group genes Psc and Su(z)2 maintain somatic stem cell identity and activity in Drosophila.
    Fuller MT
    PloS one 7.12 (2012): e52892.

    Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
    Brahmachari V
    Genes to cells : devoted to molecular & cellular mechanisms 15.7 (2010 Jun): 725-35.

    The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning.
    Renkawitz R
    The EMBO journal 26.19 (2007 Oct 3): 4203-14.

    Comparative analysis of Hox downstream genes in Drosophila.
    Lohmann I
    Development (Cambridge, England) 134.2 (2007 Jan): 381-92.

    Drosophila Hox and sex-determination genes control segment elimination through EGFR and extramacrochetae activity.
    Sánchez-Herrero E
    PLoS genetics 8.8 (2012): e1002874.

    Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function.
    Hombría JC
    PLoS genetics 9.2 (2013): e1003252.

    Polycomb group genes Psc and Su(z)2 restrict follicle stem cell self-renewal and extrusion by controlling canonical and noncanonical Wnt signaling.
    Xi R
    Genes & development 24.9 (2010 May): 933-46.

    Transcriptional regulation of Drosophila gonad formation.
    Renault AD
    Developmental biology 392.2 (2014 Aug 15): 193-208.

    Bithorax-complex genes sculpt the pattern of leucokinergic neurons in the Drosophila central nervous system.
    Díaz-Benjumea FJ
    Development (Cambridge, England) 140.10 (2013 May): 2139-48.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Drosophila retinal pigment cell death is regulated in a position-dependent manner by a cell memory gene.
    Théodore L
    The International journal of developmental biology 52.1 (2008): 21-31.

    Female-biased dimorphism underlies a female-specific role for post-embryonic Ilp7 neurons in Drosophila fertility.
    Allan DW
    Development (Cambridge, England) 140.18 (2013 Sep): 3915-26.

    Transcription in the absence of histone H3.2 and H3K4 methylation.
    Basler K
    Current biology : CB 22.23 (2012 Dec 4): 2253-7.

    Hox gene cross-regulatory interactions in the embryonic brain of Drosophila.
    Hirth F
    Mechanisms of development 121.6 (2004 Jun): 527-36.

    Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila.
    Mann RS
    Development (Cambridge, England) 140.9 (2013 May): 2027-38.

    Functional characterization of the dRYBP gene in Drosophila.
    Busturia A
    Genetics 179.3 (2008 Jul): 1373-88.

    The molecular genetics of the bithorax complex of Drosophila: cis-regulation in the Abdominal-B domain.
    Lewis EB
    The EMBO journal 9.13 (1990 Dec): 4277-86.

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