Antigen: Abdominal-B homeobox protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Celniker, S.
Host Species: mouse
Depositors Institution: Genome Dynamics, Lawrence Berkeley National Lab
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Immunohistochemistry: spatial distribution in 1) wild-type embryos [Genes Dev. 3, 1424-1436], 2) Abd-B mutant embryos [EMBO J. 9, 4277-4286], and 3) Transabdominal mutant wing discs [PNAS USA 90, 1566-1570].
Antigen Molecular Weight: Predicted: 56 and 30kDa; Apparent: 54 and 36kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Drosophila Abd-B homeodomain/fusion protein trpE/Abd-B fusion protein semi-purified from whole-cell lysates by acrylamide gel electrophoresis
Alternate Gene Names: bex; BX-C; DmAbdB; Dmel\CG11648; Droabdb; FAB;
Alternate Antibody Name: anti-ABD-B; anti-ABDB; 1A2E9
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: a.a. 270-376
Uniprot ID: P09087
Immunogen Sequence: 270-376 aa region of isoform M
Entrez Gene ID: 47763
Antibody Registry ID: AB_528061
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-ABD-B (1A2E9) was deposited to the DSHB by Celniker, S. (DSHB Hybridoma Product anti-ABD-B (1A2E9))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References
Gel Supershift References